Estuarine sediments are key hotspots of intracellular and extracellular antibiotic resistance genes: A high-throughput analysis in Haihe Estuary in China

Zhao, Ze; Zhang, Kai; Wu, Nan; Li, Wenjie; Wang, Xu; Zhang, Ying; Niu, Zhiguang

doi:10.1016/j.envint.2019.105385

Cited by 70 publications

(31 citation statements)

References 63 publications

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“…These findings suggest the widespread of sulfonamide and tetracycline resistance genes in the environment which is consistent with the results presented by a previous study by Hao et al, (2019) [ 19 ]. The authors also proposed that abiotic selective stressors such as antibiotics and heavy metal might influence the relative abundance of eARGs in the environment and this finding was corroborated by a previous study [ 61 ]. In this study, the relative abundance of eARGs in activated sludge was found to be high.…”

Section: Edna As Sources Of Antibiotic Resistance In the Environmesupporting

confidence: 68%

“…Dong et al, 2019, reported different eARGs ( sul1 , sul2 , tetW , tetX , ermA , ermB , bla TEM , ampC , cat and cmr ) and class I integron (intI1) in the sludge samples collected from hospitals, pharmaceutical industries, WWTP, swine manure and sediment in an urban lakes [ 18 ]. The results indicated that eARGs is usually lower than that of iARGs in sediments, and this finding was contrasted with the study, which was performed in estuary sediments, where the abundance of eARGs was higher [ 61 ]. Interestingly, the authors also found the highest ratio of eARGs in hospital sludge, followed by urban lake sediment, reflecting that hospital sludge could be an ideal pool for eARGs.…”

Section: Edna As Sources Of Antibiotic Resistance In the Environmementioning

confidence: 59%

“…Likewise, the abundance of eARGs was reported in estuary sediments from China [ 61 ]. This study showed that estuary sediments are also one of the reservoirs of eARGs.…”

Section: Edna As Sources Of Antibiotic Resistance In the Environmementioning

confidence: 99%

“…This study showed that estuary sediments are also one of the reservoirs of eARGs. Besides, the authors observed the high diversity and abundance of eARGs ( IntI-1( clinic), aad A-01, aad A1, aad A2-03, tet G-01, tet X, qacE delta1-01 and qac H-01) which may be attributed to the high frequency of HGT to disseminate ARGs in the competent bacteria [ 61 ]. It was observed that the abundance of eARGs was relatively higher than that of iARGs in sediments.…”

Section: Edna As Sources Of Antibiotic Resistance In the Environmementioning

confidence: 99%

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Extracellular DNA (eDNA): Neglected and Potential Sources of Antibiotic Resistant Genes (ARGs) in the Aquatic Environments

Sivalingam

Poté

Prabakar³

2020

Pathogens

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Over the past decades, the rising antibiotic resistance bacteria (ARB) are continuing to emerge as a global threat due to potential public health risk. Rapidly evolving antibiotic resistance and its persistence in the environment, have underpinned the need for more studies to identify the possible sources and limit the spread. In this context, not commonly studied and a neglected genetic material called extracellular DNA (eDNA) is gaining increased attention as it can be one of the significant drivers for transmission of extracellular ARGS (eARGs) via horizontal gene transfer (HGT) to competent environmental bacteria and diverse sources of antibiotic-resistance genes (ARGs) in the environment. Consequently, this review highlights the studies that address the environmental occurrence of eDNA and encoding eARGs and its impact on the environmental resistome. In this review, we also brief the recent dedicated technological advancements that are accelerating extraction of eDNA and the efficiency of treatment technologies in reducing eDNA that focuses on environmental antibiotic resistance and potential ecological health risk.

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Section: Edna As Sources Of Antibiotic Resistance In the Environmesupporting

confidence: 68%

Section: Edna As Sources Of Antibiotic Resistance In the Environmementioning

confidence: 59%

“…Likewise, the abundance of eARGs was reported in estuary sediments from China [ 61 ]. This study showed that estuary sediments are also one of the reservoirs of eARGs.…”

Section: Edna As Sources Of Antibiotic Resistance In the Environmementioning

confidence: 99%

Section: Edna As Sources Of Antibiotic Resistance In the Environmementioning

confidence: 99%

See 2 more Smart Citations

Extracellular DNA (eDNA): Neglected and Potential Sources of Antibiotic Resistant Genes (ARGs) in the Aquatic Environments

Sivalingam

Poté

Prabakar³

2020

Pathogens

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“…Absolute abundance of 16S-rRNA gene copies fluctuated from 3.04×10 4 to 8.41×10 5 copies/L in water, 2.11×10 7 to 5.27×10 9 copies/g in the biofilm and 4.31×10 7 to 4.73×10 8 copies/g in the sediments. Zhao (2020) reported similar levels of 16S-rRNA gene (9.06×10 6 to 2.93×10 8 copies/L) in sediment from Haihe Estuary [22]. Compared with other river sediments, 16S-rRNA gene concentrations were lower than those…”

Section: Args In Weihe-river Ecosystemmentioning

confidence: 95%

Prevalence and pollution characteristics of antibiotic resistant genes in one high anthropogenically-impacted river

Qing-zhao

Zhang

2020

PLoS ONE

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The objectives of this study were to comprehensively investigate the occurrence, distribution, and mobility of antibiotic resistant genes (ARGs) in the biofilm, water, and sediment from a section of the Weihe-river, in the northern Henan province, China. The abundances of nine ARGs belonging to four commonly used antibiotic classes (tetracyclines, sulfonamides, fluoroquinolones, and multidrug) and class 1 integron-integrase gene (intI1) were quantified. Sulfonamides gene (sulI) accounted for the highest percentage of detected ARGs in most sampling sites, including in water, biofilm, and sediment. Among the resistance genes, IntI1 and sul1 were significantly correlated (r>0.800, p<0.01) with a fecal coliform (FC) detected in the biofilm, and there was also a significantly positive correlation between the abundances of 16SrRNA and intI1 in the biofilms. Compared with the sediment and water samples, the biofilms contained sufficient nutrients to promote bacterial reproduction. Under sufficient total nitrogen and phosphorus concentrations, the horizontal gene transfer due to intI1 plays a key role in the formation and migration of ARGs within biofilms.

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Effects of antibiotic resistance genes on health risks of rivers in habitat of wild animals under human disturbance – based on analysis of antibiotic resistance genes and virulence factors in microbes of river sediments

Hu,

Ren,

Liang

et al. 2024

Ecology and Evolution

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Studying the ecological risk of antibiotic resistance genes (ARGs) to wild animals from human disturbance (HD) is an important aspect of “One Health”. The highest risk level of ARGs is reflected in pathogenic antibiotic‐resistant bacteria (PARBs). Metagenomics was used to analyze the characteristics of PARBs in river sediments. Then, the total contribution of ARGs and virulence factors (VFs) were assessed to determine the health risk of PARBs to the rivers. Results showed that HD increased the diversity and total relative abundance of ARG groups, as well as increased the kinds of PARBs, their total relative abundance, and their gene numbers of ARGs and VFs. The total health risks of PARBs in wild habitat group (CK group), agriculture group (WA group), grazing group (WG group), and domestic sewage group (WS group) were 0.067 × 10−3, −1.55 × 10−3, 87.93 × 10−3, and 153.53 × 10−3, respectively. Grazing and domestic sewage increased the health risk of PARBs. However, agriculture did not increase the total health risk of the rivers, but agriculture also introduced new pathogenic mechanisms and increased the range of drug resistance. More serious was the increased transfer risk of ARGs in the PARBs from the rivers to wild animals under agriculture and grazing. If the ARGs in the PARBs are transferred from the rivers under HD to wild animals, then wild animals may face severe challenges of acquiring new pathogenic mechanisms and developing resistance to antibiotics. Further analysis showed that the total phosphorus (TP) and dissolved organic nitrogen (DON) were related to the risk of ARGs. Therefore, controlling human emissions of TP and DON could reduce the health risk of rivers.

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Estuarine sediments are key hotspots of intracellular and extracellular antibiotic resistance genes: A high-throughput analysis in Haihe Estuary in China

Cited by 70 publications

References 63 publications

Extracellular DNA (eDNA): Neglected and Potential Sources of Antibiotic Resistant Genes (ARGs) in the Aquatic Environments

Extracellular DNA (eDNA): Neglected and Potential Sources of Antibiotic Resistant Genes (ARGs) in the Aquatic Environments

Prevalence and pollution characteristics of antibiotic resistant genes in one high anthropogenically-impacted river

Effects of antibiotic resistance genes on health risks of rivers in habitat of wild animals under human disturbance – based on analysis of antibiotic resistance genes and virulence factors in microbes of river sediments

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