Prevalence and pollution characteristics of antibiotic resistant genes in one high anthropogenically-impacted river

Qing-zhao, LI; Zhang, Qiuling

doi:10.1371/journal.pone.0231128

Cited by 9 publications

(6 citation statements)

References 26 publications

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“…In a recent study on the three rivers in Nepal (the Sundarijal, Thapathali, and Chovar Rivers), the abundance levels of the intI1 gene were measured in the range of 15-5 × 10 6 copies/mL, which is slightly lower than that in the Saigon River in Ho Chi Minh City [37]. The level of the intI1 gene was observed to associate with the abundance of ARG over time in anthropogenically-impacted rivers and to significantly correlate with human pathogens in a wastewater treatment plant [14,60,63,64]. The high abundance of three integrons (intI1, intI2, and intI3), and the target ARG genes found in the Saigon River impacted by various human activities likely indicates potential widespread antibiotic resistance across the bacterial community in the aquatic environment via horizontal gene transfer.…”

Section: Occurrences Of Args and Integrons In The Saigon Rivermentioning

confidence: 95%

“…Efflux pumps are transport proteins capable of moving toxic substances (e.g., antibiotics and heavy metals) out of the bacterial cells and they play a major role in both intrinsic and acquired multidrug resistance [58,59]. In a recent study, Wei Hou et al reported a high abundance and diversity of genes encoding efflux pumps in nine lake and reservoirs in the north of China and suggested that efflux pumps are likely a predominant resistance mechanism in natural water bodies [60]. In this study, the occurrence of the efflux pump gene oqxB in the surface water was found to range from 53 to 4.6 × 10 5 copies/mL (Figure 1, Table S3).…”

Section: Occurrences Of Args and Integrons In The Saigon Rivermentioning

confidence: 99%

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Prevalence of Antibiotic Resistance Genes in the Saigon River Impacted by Anthropogenic Activities

Truong

Hoang²,

Tran

et al. 2021

Water

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Despite of a high abundance of antibiotics, heavy metals, and organic matters detected in the Saigon River in Ho Chi Minh City, the level and spread of antibiotic resistance genes in this river are poorly understood. In this study, total 10 antibiotic resistance genes (ARGs), including genes conferring resistance to aminoglycosides (aac(6)-Ib-cr), β-lactam antibiotics (blaCTX-M, blaSHV, blaTEM), quinolones (qnrA, qnrB), sulfonamides (sul1, sul2), trimethoprim (dfrA), efflux pump (oqxB), and three genes of genetic elements, including integron classes 1, 2, and 3 (intI1, intI2, intI3), are quantified by qPCR. Water samples were collected from the industrial, agricultural, residential, and less impacted areas for the wet and dry seasons. The results present high occurrence rates for 10 ARGs that were observed in all the sampling sites with the following order: sul1, sul2, dfrA > aac(6)-Ib-cr > blaCTX-M, blaSHV, blaTEM > qnrA, qnrB. Although the levels of ARGs and integrons in the dry season were found about to be about one order of magnitude higher than those in the wet season, the exact mechanisms for this are not fully clear. The correlation analysis presented here suggests that the contamination of organic matter and nutrients from agricultural, industrial, and residential activities likely contributes to the prevalence of ARGs, integrons, total bacterial load, and the potential development and spread of antibiotic resistance in the aquatic environments considered here.

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Section: Occurrences Of Args and Integrons In The Saigon Rivermentioning

confidence: 95%

Section: Occurrences Of Args and Integrons In The Saigon Rivermentioning

confidence: 99%

Prevalence of Antibiotic Resistance Genes in the Saigon River Impacted by Anthropogenic Activities

Truong

Hoang²,

Tran

et al. 2021

Water

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“…Some previous works had already pointed out the presence of pharmaceuticals (including AZM, CPR, CLR, LVX, NFX, SMX and TMP) (Aubertheau et al, 2017;Auguet et al, 2017;Carles et al, 2021;Valdés et al, 2021) and MGEs (integrons) in similar samples (Aubertheau et al, 2017;Auguet et al, 2017;Chen et al, 2020;Li and Zhang, 2020). However, it was not clear how the presence of integrons can be linked to the presence of ATBs, as integrons also contain genes other than ARGs or encoding proteins of unknown function.…”

Section: What Potential Risk Does River Biofilm Contamination Pose?mentioning

confidence: 99%

River Biofilms Microbiome and Resistome Responses to Wastewater Treatment Plant Effluents Containing Antibiotics

et al. 2022

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Continuous exposure to low concentrations of antibiotics (sub-minimal inhibitory concentration: sub-MIC) is thought to lead to the development of antimicrobial resistance (AMR) in the environmental microbiota. However, the relationship between antibiotic exposure and resistance selection in environmental bacterial communities is still poorly understood and unproven. Therefore, we measured the concentration of twenty antibiotics, resistome quality, and analyzed the taxonomic composition of microorganisms in river biofilms collected upstream (UPS) and downstream (DWS) (at the point of discharge) from the wastewater treatment plant (WWTP) of Poitiers (France). The results of statistical analysis showed that the antibiotic content, resistome, and microbiome composition in biofilms collected UPS were statistically different from that collected DWS. According to Procrustes analysis, microbial community composition and antibiotics content may be determinants of antibiotic resistance genes (ARGs) composition in samples collected DWS. However, network analysis showed that the occurrence and concentration of antibiotics measured in biofilms did not correlate with the occurrence and abundance of antibiotic resistance genes and mobile genetic elements. In addition, network analysis suggested patterns of co-occurrence between several ARGs and three classes of bacteria/algae: Bacteroidetes incertae sedis, Cyanobacteria/Chloroplast, and Nitrospira, in biofilm collected UPS. The absence of a direct effect of antibiotics on the selection of resistance genes in the collected samples suggests that the emergence of antibiotic resistance is probably not only due to the presence of antibiotics but is a more complex process involving the cumulative effect of the interaction between the bacterial communities (biotic) and the abiotic matrix. Nevertheless, this study confirms that WWTP is an important reservoir of various ARGs, and additional efforts and legislation with clearly defined concentration limits for antibiotics and resistance determinants in WWTP effluents are needed to prevent their spread and persistence in the environment.

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“…Гени резистентності не лише залишаються в різних природних матрицях, але й мігрують, трансформуються, поширюються і дифундують в інші біотопи навколишнього середовища. І, як результат, вони дестабілізують навколишнє середовище, потрапляють в харчовий ланцюг, впливають на здоров'я людини (Li, Zhang, 2020). Тому інформаційно-просвітницька робота, проведення моніторингу антибіотикорезистентної мікробіоти у ґрунтовій та водній екосистемах є життєво важливими заходами, що спрямовані на обмеження поширення забруднення навколишнього середовища та збереження біорізноманітності, екосистеми та здоров'я людини.…”

Section: висновкиunclassified

Antibiotic-resistant microbiota of water and soil ecosystems as risk factors for human health

Matylonok

Pakhomov

Поліщук

2021

ean

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Bacterial resistance to antibiotics is one of the three major health challenges of the 21st century. One of the most important reasons for the acquisition and spread of antibiotic resistance in the environment is the irrational and uncontrolled use of antibacterial drugs, not only for medical but also other purposes, and their improper disposal. The microbiome of aquatic and soil ecosystems is characterized by the acquisition of antibiotic resistance through mobile genetic elements, contact with antibacterial drugs and their residues, the action of heavy metals and environmental stress. Also, according to the literature, it is noted that the resistance of microorganisms to antibacterial drugs in the environment existed much earlier than in clinical strains. These facts can not help but worry, because antibiotic-resistant strains of the environment have an extremely negative impact on human health. Once in the human body with water and food, they significantly complicate and / or make it impossible to further treat life-threatening diseases. Also, antibacterial residues circulating in aquatic and soil ecosystems, entering the human body can cause cancer, allergic reactions or disruption of the natural intestinal microflora. These ecosystems are characterized by large-scale spread of antibiotic-resistant microorganisms, antibacterial drugs and their residues. The aim of our work was to analyze with the help of theoretical methods of scientific research the reasons for the acquisition and spread of antibiotic resistance among environmental microbiota, namely in aquatic and soil ecosystems. To determine the impact of antibiotic-resistant bacteria of these ecosystems on human health. We have found that antibacterial drugs, antibiotic-resistant strains and resistance genes are a particular problem for wastewater treatment. Antibiotics can provide a selective load, as the mechanisms that break them down can promote resilience and selectively enrich. Wastewater treatment plants can be a favorable factor for the horizontal transfer of genes and the development of bacterial polyresistance, and high-resistance genes can be preserved even after disinfection. Soil is also an important reservoir for antibiotic-resistant bacteria and resistance genes. Microorganisms are in a constant struggle for existence in this ecosystem and try to colonize the micro-scale with the most favorable for their ecotype habitat. Antibiotic-resistant soil bacteria are in close contact with other members of the microbiota, which in turn promotes the horizontal transfer of resistance genes, even between cells of different species or genera through genetic determinants. Conclusion: ecosystems are characterized by large-scale spread of antibiotic-resistant microorganisms, antibacterial drugs and their residues. Therefore, this problem should be properly addressed, as the presence of antibiotic-resistant microorganisms, antibacterial drugs and their residues in the environment can cause unpredictable environmental consequences and adversely affect human health with more severe incurable infectious diseases. Monitoring programs for antibiotic-resistant microorganisms and resistance genes in soil and aquatic ecosystems are necessary and very relevant today. After all, this microbiota poses a serious threat to both the environment and human health and can easily spread from one part of the world around the world.

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Prevalence and pollution characteristics of antibiotic resistant genes in one high anthropogenically-impacted river

Cited by 9 publications

References 26 publications

Prevalence of Antibiotic Resistance Genes in the Saigon River Impacted by Anthropogenic Activities

Prevalence of Antibiotic Resistance Genes in the Saigon River Impacted by Anthropogenic Activities

River Biofilms Microbiome and Resistome Responses to Wastewater Treatment Plant Effluents Containing Antibiotics

Antibiotic-resistant microbiota of water and soil ecosystems as risk factors for human health

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