Estudio comparativo de los venenos de serpiente Cascabel (Crotalus durissus durissus) de ejemplares adultos y recien nacidos

Lomonte, Bruno; Gené, JoséA.; Gutiérrez, José Marı́a; Cerdas, Luis

doi:10.1016/0041-0101(83)90094-6

Cited by 29 publications

(12 citation statements)

References 10 publications

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“…For rattlesnakes these families are predominately the enzymatic L-amino acid oxidases (LAAO), phosphodiesterases (PDE), metalloproteinases (SVMP), serine proteases (SVSP), and phospholipases A 2 (PLA 2 ), and the non-enzymatic myotoxin a homologs, disintegrins, cysteine-rich secretory proteins (CRiSPs), and C-type lectins [ 15 , 16 ]. Rattlesnake venoms have been shown to vary in composition as a function of species [ 13 , 15 , 17 ], age [ 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ], and geography [ 10 , 28 , 29 , 30 ]. Several other factors, such as season and sex, may also affect individual venom composition [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Venom Ontogeny in the Mexican Lance-Headed Rattlesnake (Crotalus polystictus)

Mackessy

Leroy

Mociño-Deloya

et al. 2018

Toxins

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As trophic adaptations, rattlesnake venoms can vary in composition depending on several intrinsic and extrinsic factors. Ontogenetic changes in venom composition have been documented for numerous species, but little is known of the potential age-related changes in many rattlesnake species found in México. In the current study, venom samples collected from adult and neonate Crotalus polystictus from Estado de México were subjected to enzymatic and electrophoretic analyses, toxicity assays (LD50), and MALDI-TOF mass spectrometry, and a pooled sample of adult venom was analyzed by shotgun proteomics. Electrophoretic profiles of adult males and females were quite similar, and only minor sex-based variation was noted. However, distinct differences were observed between venoms from adult females and their neonate offspring. Several prominent bands, including P-I and P-III snake venom metalloproteinases (SVMPs) and disintegrins (confirmed by MS/MS) were present in adult venoms and absent/greatly reduced in neonate venoms. Age-dependent differences in SVMP, kallikrein-like, phospholipase A2 (PLA2), and L-amino acid oxidase (LAAO) activity levels were confirmed by enzymatic activity assays, and like many other rattlesnake species, venoms from adult snakes have higher SVMP activity than neonate venoms. Conversely, PLA2 activity was approximately 2.5 × greater in venoms from neonates, likely contributing to the increased toxicity (neonate venom LD50 = 4.5 μg/g) towards non-Swiss albino mice when compared to adult venoms (LD50 = 5.5 μg/g). Thrombin-like (TLE) and phosphodiesterase activities did not vary significantly with age. A significant effect of sex (between adult male and adult female venoms) was also observed for SVMP, TLE, and LAAO activities. Analysis of pooled adult venom by LC-MS/MS identified 14 toxin protein families, dominated by bradykinin-inhibitory peptides, SVMPs (P-I, P-II and P-III), disintegrins, PLA2s, C-type-lectins, CRiSPs, serine proteinases, and LAAOs (96% of total venom proteins). Neonate and adult C. polystictus in this population consume almost exclusively mammals, suggesting that age-based differences in composition are related to physical differences in prey (e.g., surface-to-volume ratio differences) rather than taxonomic differences between prey. Venoms from adult C. polystictus fit a Type I pattern (high SVMP activity, lower toxicity), which is characteristic of many larger-bodied rattlesnakes of North America.

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Section: Introductionmentioning

confidence: 99%

Venom Ontogeny in the Mexican Lance-Headed Rattlesnake (Crotalus polystictus)

Mackessy

Leroy

Mociño-Deloya

et al. 2018

Toxins

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“…Diversity in venom composition and biological activities has also been associated with the age of snakes in C. durissus durissus (6,12,13). Moreover, in this species toxicity appears to be induced in part by crotoxin, a potent neurotoxin present in its venom in large quantities (14).…”

Section: Introductionmentioning

confidence: 99%

Biochemical comparison of venoms from young Colombian Crotalus durissus cumanensis and their parents

Céspedes

Castro

Jimenez³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

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ABSTRACT:Crotalus durissus cumanensis, a rattlesnake endemic to Colombia and Venezuela, is considered one of the most lethal snake species in Latin America. The aim of the present study was to compare the protein content and biological activity of the venom obtained from eight specimens of C. durissus cumanensis, namely two adults from different localities of Colombia and six offspring born in captivity. Protein profiles of crude venoms were analyzed by SDS-PAGE and RP-HPLC, and biological activities were evaluated for lethality, edema, defibrination, hemolytic and coagulant activities to assess individual venoms of adults and a pool of young snake venoms. Transient edema appeared rapidly after venom inoculation, whereas hemorrhagic effect was not observed. Differences in protein profiles, lethality, hemolytic, coagulant and defibrinating activities between both adult snake venoms were observed; those from the mother snake exhibited higher activities. Venoms from young snakes were similar to the one obtained from the mother, but the coagulant effect was stronger in offspring venoms. Notably, biological effects of the father snake venom were not comparable to those previously described for C. durissus cumanensis from Venezuela and C. durissus terrificus from Brazil, confirming the high variability of the venom from Crotalus species.

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“…Desta forma, o controle de qualidade desses venenos tem sido objeto de intensa investigação há muito tempo, vista a sua utilização na produção de anticorpos para tratamento no caso de envenenamento. Lachesis muta apresenta pouca toxicidade para mamíferos (GUTIÉRREZ et al, 1990;GUTIÉRREZ et al, 1991;LOMONTE et al, 1983) , 1999;GUÉRCIO et al, 2006;SALDARRIAGA et al, 2003;ZELANIS et al, 2007). Figura 3.…”

Section: 51unclassified

Estudo e caracterização bioquímica e biológica do veneno de serpentes jovens e adultas da espécie >i<Bothrops moojeni>/i<

Hatakeyama¹

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Aos meus amigos de toda a vida, os mais presentes e os não tão presentes por motivos de trabalho ou simplesmente por morar longe. Obrigada, Gustavo, Hiago, Giovane, Marcelo e Ana Gabriela. Em especial ao Gustavo por ser quem é, pelo companheirismo que temos mesmo não sendo tão presente fisicamente, por como nossa amizade evoluiu desde que nos conhecemos, há mais de 10 anos, e também por ser quem me apresentou o Hiago, outra figura ímpar na minha vida, com quem compartilhei momentos especiais mesmo com seu gênio difícil de lidar e com um ego tão inflado quanto o maior balão que já voou no céu. São as amizades que ganhei fora do meio acadêmico e são duas das que mais considero. Aos dois, agradeço do fundo de coração todo o carinho. Amo vocês! À Dra. Kathleen Fernandes Grego, Diretora do Laboratório de Herpetologia do Instituto Butantan, por permitir a realização deste trabalho e por estar sempre disposta a nos ajudar em todos os aspectos. Ao Dr. Sávio Stefanini Sant'Anna, do Laboratório de Herpetologia do Instituto Butantan, pela extração de venenos das serpentes. A todos do laboratório de Herpetologia pelo apoio, companheirismo, simpatia e por proporcionar a este lugar um ambiente tão agradável para se trabalhar e se conviver diariamente.

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Estudio comparativo de los venenos de serpiente Cascabel (Crotalus durissus durissus) de ejemplares adultos y recien nacidos

Cited by 29 publications

References 10 publications

Venom Ontogeny in the Mexican Lance-Headed Rattlesnake (Crotalus polystictus)

Venom Ontogeny in the Mexican Lance-Headed Rattlesnake (Crotalus polystictus)

Biochemical comparison of venoms from young Colombian Crotalus durissus cumanensis and their parents

Estudo e caracterização bioquímica e biológica do veneno de serpentes jovens e adultas da espécie >i<Bothrops moojeni>/i<

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