Estudo comparativo da biocompatibilidade da submucosa intestinal porcina e pericárdio bovino usados como enxertos na veia cava de cães

Greca, Fernando Hintz; Noronha, Lúcia de; Costa, Francisco Diniz Afonso da; Filho, Zacarias Alves de Souza; Soccol, Andréa Thomaz; Feres, André Nery; Duda, João Ricardo; Adams, Eduardo

doi:10.1590/s0102-86502005000400010

Cited by 8 publications

(10 citation statements)

References 12 publications

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“…The major complications with the use of the patches are similar to those of the other kind of arterial substitutes: thrombosis, rupture or pseudoaneurysm formation, and operatory wound infection. [20][21][22][23][24][25][26][27] The rate of aneurismal formation and resistance to rupture of bovine pericardium, Dacron, PTFE, and greater saphenous vein have been reported in the literature. Biasi et al, in 2002, reported no cases of rupture or aneurismal formation by the use of bovine pericardium patch angioplasty during carotid endarterectomy in 323 cases.…”

Section: Discussionmentioning

confidence: 99%

Sugarcane biopolymer patch in femoral vein angioplasty on dogs

Barros-Marques

Marques-Lins

Albuquerque

et al. 2012

Journal of Vascular Surgery

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The sugarcane biopolymer membrane is easily synthesized with a low cost of production. This membrane has been used in many areas of experimental surgery as in the healing of skin wounds, in urinary reconstruction, in reconstruction of tympanic membrane, and as an arterial substitute, but there is no report of its use as a vein substitute. In order to evaluate the possibility of using the sugarcane biopolymer membrane in venous reconstructive surgery, this study analyzed its utilization in femoral vein patch angioplasty.

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Section: Discussionmentioning

confidence: 99%

Sugarcane biopolymer patch in femoral vein angioplasty on dogs

Barros-Marques

Marques-Lins

Albuquerque

et al. 2012

Journal of Vascular Surgery

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“…Todos os animais que receberam implantes tratados com soluções de glutaraldeído apresentaram angiogênese em maior intensidade do que o observado nos grupos tratados com glicerina, demonstrando que a utilização do glutaraldeído induz à inflamação crônica e fibrose [7]. Este tipo de inflamação se caracteriza por uma infiltração de células mononucleares amplamente encontradas no presente estudo, que incluem macrófa-gos, células gigantes, linfócitos e plasmócitos.…”

Section: Discussionunclassified

“…No entanto, alguns esporos de bactérias e vírus de incubação lenta não foram afetados pelo tratamento com estas concentrações [10] sendo a concentração de 0,625% ineficiente à inativação do vírus rábico [23]. O aumento nas concentrações do glutaraldeído poderia eliminar os diferentes agentes infecciosos, entretanto, estas provocam reações inflamatórias adversas devido à formação de uma cápsula fibrosa ao redor do implante [7] inflamação crônica [21], e a calcificação [1,4,11].…”

Section: Introductionunclassified

Pathological Features of Bovine Pericardium Implanted Into Mice Abdominal Wall after Preservation with Glutaraldehyde or Glycerin

Costa

Silva²,

Andrade

2016

Acta Scientiae. Vet.

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Background: Lately, the use of biological materials has been widely indicated in surgical procedures to restore structure and function of injured tissues. Bioimplants require different conservation techniques; among these, glutaraldehyde preservation stands out owing to its higher antimicrobial efficiency as compared to glycerin. In view of the need to determine a concentration of glutaraldehyde that can act as a biocide but do not cause undesirable tissue reactions, this study aimed to identify and quantify gross and microscopic tissue alterations after implantation of bovine pericardium, which was preserved in various concentrations of glutaraldehyde, in the abdominal wall of mice.Materials, Methods & Results: Fresh pericardia from 18 bovines were fractioned into 1cm2 samples and treated with a 98% glycerin solution for 30 days (control group), or 0.625%, 1%, and 1.5% glutaraldehyde solution for 18 days (experimental groups). An abdominal muscle fragment was excised from each mouse, and a 1-cm2 fragment of preserved pericardium was implanted in the area. Sixty mice (n = 15 per treatment) divided into groups were observed for 7, 14, and 30 days, and five animals from each group were euthanized at each time point for gross and microscopic examination. Fragments of the implants and adjacent skin lesions were harvested, fixed in formalin, and processed for routine histology and microscopic analysis. Both the type of inflammatory infiltrate and the repair process of the tissue response were similar between the groups that received glycerin-preserved pericardium and those that were subjected to pericardium preserved with 0.625% glutaraldehyde. Animals that received 1% glutaraldehyde-preserved implants and were examined 30 days thereafter exhibited a chronic, intense reaction with fibrosis and necrosis of the abdominal wall muscles, as well as calcification and presence of giant cells, when compared to the animals examined at 7 and 14 days in the same treatment group. These changes were also present and more intense in animals that received 1.5% glutaraldehyde-preserved pericardium examined at 14 and 30 days later, with tissue destruction and impaired incorporation of the implant into the adjacent muscle tissue.Discussion: The continuous cell destruction observed in animals treated with implants preserved with 1% or 1.5% glutaraldehyde is a hallmark of chronic inflammation, since several inflammatory cell molecules contribute to this lesion. A cycle is created: continuous degradation sustains inflammation, and inflammatory molecules contribute to the process of cell destruction. Consequently, we conclude that the use of glutaraldehyde at concentrations of 1 or 1.5% is not feasible for preservation of biological materials. Tissue repair was chronologically more effective in the group treated with glycerinpreserved implants, since animals treated with glutaraldehyde-preserved implants needed a longer period to restore due to presence of a persistent inflammatory response, immunogenicity, calcification, and deficient remodeling. The ideal preservative for biological materials should not cause chronic and/or intense inflammatory reaction in order to preserve the implant’s structure and allow its perfect incorporation into the tissue, even if the chosen preservative is flexible and exhibits disinfectant properties. Therefore, we conclude that glutaraldehyde at concentrations of 0.625% to 1% is suitable as a preservative for biomaterials because the tissue reaction it causes is tolerable; additionally, glutaraldehyde at concentrations close to 1% has been described to have sterilizing properties.

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“…Algunos estudios desarrollados con pericardio equino como membrana biológica demostraron que el proceso inflamatorio puede caracterizarse como crónico y moderado (96). Durante la respuesta inflamatoria crónica al implante, los productos de adherencia de células inflamatorias pueden ser generados y perjudicar el implante o reaccionar con el biomaterial y generar catabolitos tóxi-cos (97), situación que no acontece con una membrana como el pericardio homólogo, el cual, una vez empleado por Bellanzini y colaboradores (98) en la reparación de intestino grueso de equinos, recubrió las lesiones sin presencia de adherencias de otras estructuras de la cavidad abdominal, y fue recubierto e incorporado al organismo ocho semanas postimplantación, con presencia de discreto tejido cicatricial.…”

Section: Reacción Tisular Ante Un Implante De Membranas Biológicasunclassified

Implantes de membranas biológicas en cirugía reconstructiva veterinaria: aspectos básicos y métodos de conservación

Piso¹,

Restán²,

Padilla³

2015

Rev. Med. Vet.

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ResumenLas membranas biológicas se emplean como implantes en cirugía veterinaria reconstructiva con el objetivo de restablecer la función y la estructura de tejidos dañados. Diversos tejidos obtenidos de animales, conservados por diferentes técnicas e implantados en receptores de la misma o de diferente especie, permiten reparar heridas en las que es evidente la extensa pérdida tisular o la imposibilidad de inducir cicatrización por primera intención. Aunque las bondades de las membranas biológicas son mayores que sus desventajas, su uso en la rutina clínica y quirúrgica no es frecuente, en gran parte por el desconocimiento de sus características, manipulación e implantación. La presente revisión pretende recopilar los aspectos generales que envuelven las membranas biológicas, desde su obtención hasta las posibles complicaciones de su uso, a través de estudios experimentales y reportes de caso relatados con anterioridad, e incentivar su uso como biomaterial de implante en defectos anatómicos, heridas traumáticas, postoperatorios oncológicos y traumas en general.Palabras clave: animales, cirugía, membranas biológicas, reconstrucción, reparación. Biological membrane implants in veterinary reconstructive surgery: Basic aspects and conservation methods AbstractBiological membranes are used as implants in veterinary reconstructive surgery in order to restore the function and structure of damaged tissues. Various tissues from animals, kept by different techniques and implanted in recipients of the same or different species, help repair wounds where extensive tissue loss or the inability to scarring is evidenced. Although the benefits of biological membranes outweigh their disadvantages, their use in clinical and surgical routines is rare, largely due to ignorance of their characteristics, handling and implantation. The present article aims to review general aspects on biological membranes, from procurement to possible complications of their use, through experimental studies and previously reported cases, and to encourage their use as a biomaterial implant in case of anatomical defects, traumatic wounds, oncological postoperative care, and traumas in general.

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Estudo comparativo da biocompatibilidade da submucosa intestinal porcina e pericárdio bovino usados como enxertos na veia cava de cães

Abstract: SIS proved to be a functional graft patch for possessing local vena cava remodeling and its biocompatibility was similar to bovine pericardium.

Cited by 8 publications

References 12 publications

Sugarcane biopolymer patch in femoral vein angioplasty on dogs

Sugarcane biopolymer patch in femoral vein angioplasty on dogs

Pathological Features of Bovine Pericardium Implanted Into Mice Abdominal Wall after Preservation with Glutaraldehyde or Glycerin

Implantes de membranas biológicas en cirugía reconstructiva veterinaria: aspectos básicos y métodos de conservación

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