Estudo comparativo entre as metodologias preconizadas pelo CLSI e pelo EUCAST para avaliação da atividade antifúngica

Stoppa, Marcos A.; Casemiro, Luciana Assirati; Vinholis, Adriana Helena Chicharo; Cunha, Wilson Roberto; Silva, Márcio Luís Andrade e; Martins, Carlos Henrique Gomes; Furtado, Niege Araçari Jacometti Cardoso

doi:10.1590/s0100-40422009000200038

Cited by 24 publications

(11 citation statements)

References 16 publications

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“…The matrix solutions of the extracts were prepared by weighing 0.3 g of each extract and then diluting in 1 mL of DMSO. To obtain the desired concentration for testing, the extracts were further diluted in sterile distilled water so that the concentration of DMSO in the natural product did not exert any activity in the test cells (Stoppa et al, 2009).…”

Section: Methodsmentioning

confidence: 99%

Phenolic composition and medicinal usage of Psidium guajava Linn.: Antifungal activity or inhibition of virulence?

Morais-Braga

Carneiro

Machado

et al. 2017

Saudi Journal of Biological Sciences

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is a Myrtaceae plant whose medicinal properties are recognized in several locations. The use of teas and tinctures prepared from their leaves has been used to combat infections caused by fungi of the genus . In this study, aqueous extracts of leaves and hydroethanolic were tested to verify the antifungal potential and its chemical composition has been investigated. The microbiological assays were performed by broth microdilution to determine the minimum inhibitory concentration (MIC) and from these the minimum fungicidal concentration was performed (MFC) by subculturing on solid media. A cell viability curve was obtained for demonstration of inhibition of fungal growth of strains of and . Tests to check morphological changes by the action of the extracts were performed in microcultive cameras depleted environment at concentrations of MIC/2, MIC and MIC × 2. Extracts analyzed by high performance liquid chromatography demonstrated flavonoids and phenolic acids. The extracts showed fungistatic effect and no fungicide with MIC>8192 μg/mL, MFC above 8192 μg/mL. The IC was calculated ranging from 1803.02 to 5623.41 μg/mL. It has been found that the extracts affect the morphological transition capability, preventing the formation of pseudohyphae and hyphae. Teas and tinctures, therefore, have the potential antifungal, by direct contact, causing inhibition of fungal multiplication and its virulence factor, the cell dimorphism, preventing tissue invasion. Further studies are needed to elucidate the biochemical pathways and genes assets involved in these processes.

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Section: Methodsmentioning

confidence: 99%

Phenolic composition and medicinal usage of Psidium guajava Linn.: Antifungal activity or inhibition of virulence?

Morais-Braga

Carneiro

Machado

et al. 2017

Saudi Journal of Biological Sciences

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“…The product solution was prepared by weighing 0.15 g of the oil and diluting this in 1 mL of DMSO. To obtain the desired concentration for the test, the oil was further diluted in sterile distilled water (16,384 μg/mL) such that the DMSO concentration did not exert any activity on the tested cells (Stoppa et al, 2009).…”

Section: Drugs Reagents and Solution Preparationmentioning

confidence: 99%

Chemical composition, antifungal activity and potential anti-virulence evaluation of the Eugenia uniflora essential oil against Candida spp.

et al. 2018

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The development of fungal resistance to antifungal drugs has been worsening over the years and as a result research on new antifungal agents derived from plants has intensified. Eugenia uniflora L. (pitanga) has been studied for its various biological actions. In this study the chemical composition and antifungal effects of the E. uniflora essential oil (EULEO) were investigated against Candida albicans (CA), Candida krusei (CK) and Candida tropicalis (CT) standard strains. The essential oil obtained through hydro-distillation was analyzed by gas chromatography coupled to mass spectrometry (GC-MS). To determine the IC of the oil, the cellular viability curve and the inhibitory effects were measured by means of the oil's association with Fluconazole in a broth microdilution assay with spectrophotometric readings. The Minimum Fungicidal Concentration (MFC) was determined by solid medium subculture with the aid of a guide plate while the assays used to verify morphological changes emerging from the action of the fractions were performed in microculture chambers at concentrations based on the microdilution. Two major oil constituents stand out from the chemical analysis: selina-1,3,7(11)-trien-8-one (36.37%) and selina-1,3,7(11)-trien-8-one epoxide (27.32%). The concentration that reduced microorganismal growth was ≥8,192 μg/mL while the IC varied, this being between 1892.47 and 12491.80 μg/mL (oil), 10.07 - 80.78 μg/mL (fluconazole) and 18.53 - 295.60 μg/mL (fluconazole + oil). The combined activity (fluconazole + oil) resulted in indifference and antagonism. A MFC of the oil in association with fluconazole was recorded at the concentration of 8,192 μg/mL against CA and CK. The oil caused the inhibition of CA and CT morphological transition. In view of the results obtained, additional research is needed to elucidate the activity of the E. uniflora oil over genetic and biochemical processes regarding its effect on Candida spp. virulence.

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“…A difusão em disco tem sido utilizada, seguindo a norma M44-A2, onde avalia-se os halos de inibição das culturas fúngicas (CLSI, 2009). Porém, eventualmente, pesquisadores fazem modificações nas técnicas a fim de obter maior exatidão nos valores de CIM e CFM (Stoppa et al 2009). …”

Section: Resultsunclassified

Levantamento de extratos vegetais com ação anti-Candida no período de 2005-2013

Giordani

Santin²,

Cleff

2015

Rev. bras. plantas med.

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RESUMO:Entre as micoses relevantes em saúde pública destaca-se a candidíase, infecção oportunista que acomete o homem e animais. A enfermidade era considerada pouco frequente na medicina veterinária, porém relatos demonstram um aumento considerável, assim como a resistência aos antifúngicos. Com isso, pesquisas têm sido desenvolvidas visando encontrar substâncias bioativas frente ao gênero Candida. Desta forma, objetivou-se reunir dados das bases Scielo e ScienceDirect com informações entre os anos de 2005-2013 referentes à ação anti-Candida de diferentes extratos vegetais. Foi encontrado um total de 78 famílias e 208 espécies de plantas com atividade frente à Candida spp., destacando-se as famílias Asteraceae, Geraniaceae, Myrtaceae, Fabaceae, Lamiaceae, Rubiaceae, Verbenaceae e Anacardiaceae, com extratos diclorometânicos, aquosos, etanólicos, metanólico, frações e subfrações, sendo as folhas a parte vegetal mais utilizada. As plantas descritas apresentaram ação anti-Candida, porém algumas necessitam concentrações muito altas dos extratos com pequena inibição de crescimento/eliminação destas leveduras, ocorrendo variações, principalmente, quanto ao método de avaliação, tipo de extrato, parte vegetal, e procedência dos isolados fúngicos. Chama a atenção a raridade dos estudos com isolados de animais, principalmente de casos clínicos. Por fim, destacam-se as famílias Asteraceae e Geraniaceae que apresentaram maior número de espécies vegetais com atividade, podendo ser uma fonte de investigação frente à Candida spp.Palavras-chave: Plantas, leveduras, candidíase. ABSTRACT: Survey of plant extracts with anti-Candida activity in the 2005-2013 period.Among the relevant mycoses in public health, one that stands out is candidiasis, an opportunistic infection that affects humans and animals. The disease was considered uncommon in veterinary medicine, but reports show a significant increase, as well as resistance, to conventional antifungal agents. Therefore, research has been undertaken aimed at finding bioactive substances from plants that fight against Candida. Thus, the objective of this work was to gather the databases SciELO and ScienceDirect with information between the years 2005 and 2013 concerning the anti-Candida activity of different plant extracts. A total of 78 families and 208 species of plants with activity against Candida spp. was found highlighting the Asteraceae, Geraniaceae, Myrtaceae, Fabaceae, Lamiaceae, Rubiaceae, Verbenaceae and Anacardiaceae families, with dichloromethane, aqueous, ethanol and methanol extracts and fractions and subfractions, being the leaf the most used plant part. The plants described showed anti-Candida activity, but some require very high concentrations of the extracts with little growth inhibition / elimination of these yeasts, with variations related mainly to the method of assessment, type of extract, plant parts and origin of the fungal isolates. The rarity of studies with isolates from animals, mainly clinical cases, draws attention. Finally, we highlight the Asteraceae ...

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Estudo comparativo entre as metodologias preconizadas pelo CLSI e pelo EUCAST para avaliação da atividade antifúngica

Cited by 24 publications

References 16 publications

Phenolic composition and medicinal usage of Psidium guajava Linn.: Antifungal activity or inhibition of virulence?

Phenolic composition and medicinal usage of Psidium guajava Linn.: Antifungal activity or inhibition of virulence?

Chemical composition, antifungal activity and potential anti-virulence evaluation of the Eugenia uniflora essential oil against Candida spp.

Levantamento de extratos vegetais com ação anti-Candida no período de 2005-2013

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