“…Allele frequencies from Cabinda (Beleza et al, 2004), Mozambique , Equatorial Guinea (Alves et al, 2005) and Guinea-Bissau (Gonçalves et al, 2002) were used for genetic differentiation tests with São Tomé e Principe population sample, applying the Bonferroni correction for multiple tests (Hochberg, 1988). A neighbor-joining tree was constructed using the PHYLIP3.5c software package (Felsenstein, 1993) based on Reynolds Genetic distance (Reynolds et al, 1983) with a bootstrap of 10000 resamples, using population data from different Portuguese regions, Central Portugal (Manco et al 2007(Manco et al -2008, North Portugal (Pinheiro et al, 2005), Madeira , Azores (Velosa et al, 2002), and from other African populations, Cabinda (Beleza et al, 2004), Mozambique , Equatorial Guinea (Alves et al, 2005) and Guinea-Bissau (Gonçalves et al, 2002).…”