Estudo toxicológico pré-clínico agudo e determinação da CL50 do extrato bruto seco da vitex agnus castus Linn.

Barros, Jussara Diniz; Filho, Samuel Souza; Castro, Valérium Thijan Nobre de Almeida e; Torres, Vivian Mariano; Higino, Jane Sheila; Melo, Arquimedes Fernandes Monteiro de

doi:10.5216/ref.v7i3.9893

Cited by 5 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Once the extracts were obtained, they were applied in cytotoxicity bioassays against A. salina, with the aim of corroborating the effectiveness of each bioproduct and determining those with the greatest antiparasitic and/or antimicrobial potential. The lethality test is considered essential as a preliminary bioassay in the study of compounds with potential biological activity [11,13].…”

Section: Resultsmentioning

confidence: 99%

Phytochemical prospecting and cytotoxicity assays of potential bioproducts to control parasites in Colossoma macropomum Cuvier

et al. 2022

View full text Add to dashboard Cite

With the aim of determining economic and environmental alternatives that support fish farming in the State of Rondônia, a phytochemical prospection of genetic resources of the Rondônia flora was carried out. Subsequently, cytotoxicity assays against Artemia salina L. nauplii, thus observing those herbal medicines supposedly with greater potential to control parasites in fish, with emphasis on Tambaqui (C. macropomum). With regard to cytotoxicity assays, at least four herbal medicines showed a high potential, reaching values of 100% mortality of microcrustaceans, for the tested concentrations. They were extracted from Chenopodium ambrosioides (herb of Santa Maria or mastruz), Crotalaria cf. micans (maraca), Davilla sp. (fire vine, sp. 1) and Duranta sp. (during). The bioprospecting of RFG is also positioned as a conservation strategy.

show abstract

Section: Resultsmentioning

confidence: 99%

Phytochemical prospecting and cytotoxicity assays of potential bioproducts to control parasites in Colossoma macropomum Cuvier

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Esses halos foram medidos em milímetros (mm) em relação ao seu diâmetro, com auxílio de régua milimetrada. Os resultados foram submetidos à análise de variância (ANOVA) e as médias comparadas pelo teste de Tukey a 5% de probabilidade (Barbosa & Vieira 2011, Barros et al 2011.…”

Section: As Cascas Foram Acondicionadas Em Papel Craftunclassified

Atividade antibacteriana do extrato hidroalcoólico de Punica granatum Linn. sobre Staphylococcus spp. isolados de leite bovino

et al. 2014

View full text Add to dashboard Cite

Mastite bovina é caraterizada por inflamação da glândula mamária, geralmente em resposta à infecção bacteriana, compromete quali-quantitativamente a produção leiteira. Este estudo objetivou verificar a atividade antibacteriana in vitro do extrato hidroalcoólico da casca da romã sobre bactérias isoladas de leite bovino. As colônias de Staphylococcus spp. foram ressuspendidas a escala 6 de MacFarland e ajustada a sua concentração por espectrofotometria UV visível na concentração de 10 mL-1. Os extratos foram avaliados em quintuplicata, em sete concentrações: de 4mg mL-1 até 0,0625 mg.mL-1. A sensibilidade dos isolados microbianos foi determinada utilizando o teste de difusão em disco e os resultados que apresentaram zonas de inibição correspondentes a valores a partir de 15 mm, foram considerados sensíveis. Os resultados foram avaliados pelo método ANOVA, teste de Tukey 5%, utilizando o SISVAR 5.3 -DEX/UFLA. Adicionalmente o extrato foi avaliado quanto à atividade antioxidante, teores de fenóis e flavonoides totais. Para tanto o extrato foi diluído em sete concentrações: de 25 a 1000µg.mL-1, e avaliado em triplicata. O crescimento bacteriano foi inibido a partir da concentração de 4mg.mL-1 e a ação antioxidante foi verificada a partir de 50µg.mL-1, com valores correspondentes a 4.62%, atingindo platô de 64,90% na concentração de 500µg.mL-1. Na avaliação da atividade captadora de radicais, empregando o radical livre DPPH, o extrato demonstrou atividade antioxidante (IC50%= 378,80µg/mL). Porém, não foi possível correlacionar a atividade antioxidante aos teores de fenóis e flavonoides. Talvez a presença de outras substâncias alcaloides e taninos presentes no extrato, possam ter sido as responsáveis pela atividade antioxidante encontrada. Conclui-se que o extrato hidroalcoólico de Punica granatum Linn. apresenta atividade antimicrobiana contra Staphylococcus spp., demonstrando potencial benefício para o controle da mastite bovina.

show abstract

“…The vitex ( Vitex agnus‐castus L.) is a native shrub from the Mediterranean region, with lilac flowers and small fruits of brown to black color (Maia et al., 2001). These fruits are used in the cuisine of some countries due to the aroma and flavor similar to the black pepper (Barros et al., 2010). Moreover, the ethanolic extract obtained from dried vitex fruits is used in the production of herbal medicines (capsules or tinctures), mainly in Europe and the United States.…”

Section: Introductionmentioning

confidence: 99%

Production of vitex ( Vitex agnus ‐ castus L.) extract in powder form using spray‐drying: Potential for the production of functional foods

Barrientos

Maeda

Chaves

et al. 2021

J. Food Process. Preserv.

View full text Add to dashboard Cite

Vitex (Vitex agnus‐castus L.) have bioactive compounds with potential to reduce the symptoms of menopause and premenstrual syndrome, as proved by several studies. However, such compounds may have low stability, and the spicy sensation caused by vitex fruits is a challenge for application in foods. Thus, the aim of this study was to produce microparticles by spray‐drying loaded with vitex extract, and evaluate the physical and chemical properties of the powders for further application in new functional foods. The powders presented good process yield (ca. 63.4%) and retention (ca. 77.7%), volume weighted mean diameter of approximately 20.3 µm, and high stability of the bioactive compounds during the storage. Moreover, they showed good storage stability in different relative humidity, and they were classified as very low hygroscopic powders. Overall, these results are very promising and they may promote the production of a potential functional ingredient for new functional foods. Practical applications Considering the good process yield, retention and stability of the bioactive compounds during the storage, and the powder produced and characterized in the present study represents a potential functional ingredient with good technological properties. Moreover, it may be an alternative to increase the consumers’ ingestion of vitex bioactive compounds by application of the spray‐dried powder in new functional foods such as chocolate and cereal bars.

show abstract

Estudo toxicológico pré-clínico agudo e determinação da CL50 do extrato bruto seco da vitex agnus castus Linn.

Cited by 5 publications

References 0 publications

Phytochemical prospecting and cytotoxicity assays of potential bioproducts to control parasites in Colossoma macropomum Cuvier

Phytochemical prospecting and cytotoxicity assays of potential bioproducts to control parasites in Colossoma macropomum Cuvier

Atividade antibacteriana do extrato hidroalcoólico de Punica granatum Linn. sobre Staphylococcus spp. isolados de leite bovino

Production of vitex ( Vitex agnus ‐ castus L.) extract in powder form using spray‐drying: Potential for the production of functional foods

Contact Info

Product

Resources

About