Ethanol elevates intracellular calcium in rabbit parietal cells

Mutsukawa, Hiroko; Zhao, Weilu; Saitoh, Toshihito; Natomi, Hitomi; Sugano, Kentaro

doi:10.1007/bf02782828

Cited by 3 publications

(3 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Discussionsupporting

confidence: 92%

“…It is likely that ethanol stimulated AP accumulation not only through increased cAMP levels, but also by an elevation in the intracellular Ca 2+ concentration in parietal cells. Our results are supported by those of Mutsukawa et al 18 who indicated that ethanol elevates the intracellular Ca 2+ concentration in rabbit parietal cells. It should be noted that BAPTA at a concentration of 10 )4 mol/L, or even 10 )5 mol/L, resulted in a decrease in AP accumulation below the basal level in both wine-and ethanol-treated glands.…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

Wine stimulates gastric acid secretion in isolated rabbit gastric glands via two different pathways

Matsuno¹,

Tomita²,

Okabe³

2002

Aliment Pharmacol Ther

View full text Add to dashboard Cite

Background & aims: Alcoholic beverages such as beer and wine are well known to potently stimulate gastric acid secretion, most probably through an increase in circulating gastrin level. The present study examined whether or not wine stimulates gastric acid secretion by a direct effect on parietal cells, enterochromaffin‐like (ECL) cells or both. Methods: Gastric mucosa was isolated from female Japanese white rabbits and gland specimens were prepared by the collagenase digestion method. Acid secretion was assessed by gland accumulation of [14C] aminopyrine. The effects of red wine, ethanol, non‐alcoholic wine and drugs were determined by incubating gastric glands with aminopyrine. Radioactivity in solubilized glands was determined by a liquid scintillation counting. Results: Neither wine nor ethanol (diluted 1 : 102 to 1 : 104) had any effect on gastric acid secretion, whereas non‐alcoholic wine stimulated acid secretion in a dose‐dependent manner. All substances, however, significantly stimulated gastric acid secretion in IBMX (phosphodiesterase inhibitor)‐pretreated glands. S‐0509 (a CCK‐2 receptor antagonist) and atropine had no effect on acid secretion stimulated by wine, ethanol or non‐alcoholic wine in IBMX‐pretreated glands. Famotidine and omeprazole significantly inhibited the acid secretion resulting from all of the above stimulants. BAPTA (an intracellular Ca2+ chelator) inhibited acid secretion stimulated with wine or ethanol in a dose‐dependent manner, but did not inhibit secretion stimulated by non‐alcoholic wine. Conclusions: Wine was found to stimulate gastric acid secretion in gastric glands via two pathways, by an ethanol‐induced increase in the concentration of intracellular Ca2+ in parietal cells, and by histamine release from ECL cells potentially induced by constituents present in wine.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

Wine stimulates gastric acid secretion in isolated rabbit gastric glands via two different pathways

Matsuno¹,

Tomita²,

Okabe³

2002

Aliment Pharmacol Ther

View full text Add to dashboard Cite

show abstract

“…Niki et al observed similar inhibition of carbachol-and histamine-triggered proton extrusion [11]. A concomitant ethanol-induced elevation in intracellular Ca 2+ concentration was suggested, via desensitizing to the Ca 2+ response triggered by carbachol, to be responsible for the inhibitory effects of ethanol on cholinergic stimulation [17,18]. This model, however, does not explain impairment of histamine-induced acid secretion, which is mediated via the second messenger cAMP.…”

Section: Introductionmentioning

confidence: 96%

Ethanol Inhibits Gastric Acid Secretion in Rats Through Increased AMP-Kinase Activity

Kopic

Corradini

Sidani

et al. 2010

Cell Physiol Biochem

View full text Add to dashboard Cite

The effects of ethanol on gastric acid secretion remain controversial. The present study examines the effect of low-dose (2%) short term (15-20min) ethanol exposure on gastric acid secretion via a potential interaction with AMP-activated protein kinase (AMPK). Real-time fluorescence digital imaging was used to provide functional evidence for the interaction of ethanol and AMPK in modulating secretagogueinduced acid secretion. Individual rat gastric glands were loaded with the pH-sensitive dye BCECF and the secretagogues carbachol (200 µM) or histamine (200 µM) were added to induce secretion. Rates of pH recovery were calculated as ΔpH i /Δt. In one series of experiments, secretagogue-induced acid secretion was inhibited by 2% ethanol, or the AMPK activator AICAR monophosphate (AICAR) (20 mM). In a separate series, 2% ethanol was added in combination with compound C (20 µM), an AMPK inhibitor, to prevent activation of AMPK. 2% ethanol significantly suppressed stimulated acid secretion.In order to confirm modulation of AMPK activity by ethanol, the specific AMPK inhibitor compound C was used, which reversed the inhibitory effects of ethanol on stimulated acid secretion. This study demonstrates that low dose ethanol (2%) inhibits secretagoguedependent acid secretion by activation of the AMPK pathway in rat gastric parietal cells.

show abstract

Calcium-stimulated short-circuit currents in the canine proximal colonic epithelium: effects of DK-PGD₂, a metabolite of prostaglandin D₂

Larsen¹,

Schleihauf²,

Yu³

et al. 2002

Can. J. Physiol. Pharmacol.

View full text Add to dashboard Cite

Prostaglandin D2 (PGD2) has marked inhibitory effects on the canine proximal colonic epithelium set up in Ussing chambers. These effects involved a receptor that is pharmacologically distinct from the classical DP, presumably the recently identified CRTH2/DP2 variety. The mechanism underlying these effects was studied using 13,14-dihydro-15-keto-PGD2 (DK-PGD2), a stable metabolite of the parent prostanoid. The metabolite quickly reversed short circuit currents (I(sc)) stimulated by diverse agonists. Greater inhibitory effects were seen with stimulants such as carbachol and cyclopiazonic acid (CPA) rather than with forskolin or protein kinase A activators. Since the same stimulants were differentially affected by removal and replacement of serosal Ca2+, we tested the possibility that the prostanoid inhibited basolateral Ca2+ entry. In the absence of serosal Ca2+, tissues primed with CPA demonstrated concentration-dependent increases in I(sc), to cumulative additions of Ca2+ or Sr2+, though the former was more potent. Cl- removal and pretreatment with bumetanide virtually abolished responses, suggesting that the increase in I(sc) reflected Ca2+ dependent Cl- secretion. Though responses were insensitive to the L-type channel antagonist, verapamil, a marked inhibition was seen in the presence of metal cations (Gd3+, Cd2+, and La3+). Pretreatment with DK-PGD2 inhibited responses to Ca2+ in CPA-primed tissues. Thus, basolateral Ca2+ entry via store-operated Ca2+ channels may be the locus for the inhibitory effects of PGD2 in this tissue. These results could indicate a potential transduction mechanism for the novel DP receptor variously called CRTH2 or DP2.

show abstract

Ethanol elevates intracellular calcium in rabbit parietal cells

Cited by 3 publications

References 4 publications

Wine stimulates gastric acid secretion in isolated rabbit gastric glands via two different pathways

Wine stimulates gastric acid secretion in isolated rabbit gastric glands via two different pathways

Ethanol Inhibits Gastric Acid Secretion in Rats Through Increased AMP-Kinase Activity

Calcium-stimulated short-circuit currents in the canine proximal colonic epithelium: effects of DK-PGD₂, a metabolite of prostaglandin D₂

Contact Info

Product

Resources

About

Ethanol elevates intracellular calcium in rabbit parietal cells

Cited by 3 publications

References 4 publications

Wine stimulates gastric acid secretion in isolated rabbit gastric glands via two different pathways

Wine stimulates gastric acid secretion in isolated rabbit gastric glands via two different pathways

Ethanol Inhibits Gastric Acid Secretion in Rats Through Increased AMP-Kinase Activity

Calcium-stimulated short-circuit currents in the canine proximal colonic epithelium: effects of DK-PGD2, a metabolite of prostaglandin D2

Contact Info

Product

Resources

About

Calcium-stimulated short-circuit currents in the canine proximal colonic epithelium: effects of DK-PGD₂, a metabolite of prostaglandin D₂