Ethanol Exposure Suppresses Survival Kinases Activation in Adult Rat Testes

Koh, Phil-Ok

doi:10.1292/jvms.69.21

Cited by 8 publications

(8 citation statements)

References 19 publications

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“…According to our results, infertile men with alcohol consumption have high concentration of ROS, this consistent to result of [69]. ROS production, induced apoptotic cell death in testicular germ cells and suppresses 3 by suppressing the activation of survival kinases which is important for cell growth [60,61,62]. Alcohol ffect adversely on semen volume, sperm count, sperm motility and sperm morphology [63,64,65,66].…”

Section: F-a Comparison Between Semen Parameters and Mda Among Fertilsupporting

confidence: 81%

“…In our study, 8 (10.67%) of infertile men intake alcohol. Its affect fertility through interfering with function of Leydig cells, Sertoli cells, pituitary gland, ROS production, induced apoptotic cell death in testicular germ cells and suppresses spermatogenesis through increasing the activation of caspase activation of survival kinases which is important for cell growth [60,61,62]. Alcohol affect adversely on semen volume, sperm count, sperm motility and sperm morphology [63,64,65,66].…”

Section: F-a Comparison Between Semen Parameters and Mda Among Fertilmentioning

confidence: 99%

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The Seminal Plasma MDA Level as Bio-Marker for Oxidative Stress and Their Impact on Seminogram in Primary Infertile Males, Erbil-Kurdistan Region, Iraq

AL-Naqshbandi¹,

AL-Barzinji²

2017

Cihan Univ.-Erbil Sci. J.

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Male infertility is an important medical and psychosocial problem worldwide. Therefore, it is important to identify new and non-conventional factors that may play significant role in male infertility. Current study focused on detecting more risk factors and markers for primary infertility in order to develop interventions for investigation and prevent their progressions. Oxidative stress has recently been identified as an underlying mechanism of numerous sources. The aim of the present study was therefore to evaluate whether there is an association between oxidative stress and male infertility in Erbil city subjects. The present prospective case control study was carried out between March to May 2013, in Maternity Teaching Hospital/in-vitro fertilization center and private clinical laboratory. 75 infertile males represent patients group were enrolled in this study. Apparently 40 healthy individuals who have no history of clinical evidence of any disease were also joined to this study as control group. Malondialdehyde (MDA), the marker of lipid peroxidation was measured in the seminal fluid of fertile and infertile male subjects. The sperm count, sperm morphology and motility, as well as semen volume were all found significantly lower in infertile subjects compared with fertile subjects (p<0.01). In seminal plasma, the MDA level was found significantly higher in infertile group (p<0.05). Lifestyle behaviours such as varicocele repair, cigarette smoking and alcohol consumption, all enhance the generation of ROS level in the seminal plasma, such elevation associated with decreased sperm quality particularly in term of sperm count and sperm motility that leading to increase male infertility.

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Section: F-a Comparison Between Semen Parameters and Mda Among Fertilsupporting

confidence: 81%

Section: F-a Comparison Between Semen Parameters and Mda Among Fertilmentioning

confidence: 99%

The Seminal Plasma MDA Level as Bio-Marker for Oxidative Stress and Their Impact on Seminogram in Primary Infertile Males, Erbil-Kurdistan Region, Iraq

AL-Naqshbandi¹,

AL-Barzinji²

2017

Cihan Univ.-Erbil Sci. J.

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“…28 Chronic exposure to Al increases oxidative stress and decreases testosterone levels, which in turn leads to testicular atrophy, decreased seminiferous tubule diameter, and a decrease in the number of spermatogenic cells at all stages. 24,26,[29][30][31][32] Al consumption decreases reproductive activity by inhibiting spermatogenesis and causing apoptosis in germ cells. [32][33][34] Consuming high levels of dietary Ac and Al as a result of our dietary habits, starting from the prenatal period, affects the male reproductive system at different developmental stages.…”

Section: Introductionmentioning

confidence: 99%

Testicular development of male mice offsprings exposed to acrylamide and alcohol during the gestation and lactation period

2014

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Acrylamide (Ac) in the foods and alcohol (Al) in the drinks are unavoidable. Several previous studies demonstrated that these substances which are taken into the body via diet may cause adverse effects in the cells. However, there is no study about how Ac and Al may affect the male reproductive system of the offspring when consumed by the mother during pregnancy and lactation. For this purpose, sexual development in male mice was evaluated after intake of 14 mg/kg Ac and 2 g/kg Al from gestation day 6 to postnatal day (PND) 21. The weight of the offspring was reduced at birth and PND 21 for those exposed to Ac and/or Al. The gonadosomatic index of male offsprings was reduced except for the Ac-treated lactation group. Both substances induced multinuclear giant cells, degenerative cells, atrophic tubules, and maturation-arrested tubules, while decreased Leydig, Sertoli, and spermatogenic cell numbers. Lipid peroxidation level and superoxide dismutase enzyme activity increased in both Al-treated and Ac and Al-treated groups. There was only reduction in the catalase activity during the gestation and lactation periods. These findings suggest that consumption of Ac together with Al may induce impairments on testicular spermatogenesis in male offsprings.

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“…30 Activated Akt/PKB (Ser473) phosphorylate Bad at Ser136 which leads to 14-3-3 association and sequestration of Bad in the cytoplasm, away from mitochondria associated Bcl-2 family protein. [31][32][33] In this way, Akt/PKB exerts its anti-apoptotic function by neutralizing the prodeath function of Bad through 14-3-3-mediated cytoplasmic relocalization. In the present study, lupeol induces dephosphorylation of Bad at this site, trigger dissociation of 14-3-3 and enhances Bad-induced apoptosis (Fig.…”

Section: Methodsmentioning

confidence: 99%

Induction of apoptosis by lupeol in human epidermoid carcinoma A431 cells through regulation of mitochondrial, Akt/PKB and NF-kappaB signaling pathways

Prasad

Madan²,

Nigam³

et al. 2009

Cancer Biology & Therapy

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The rising incidence of skin cancer in humans makes it equivalent to malignancies of organs. Therefore, it is necessary to intensify our efforts for better understanding and development of novel treatment and preventive approaches for skin cancer. Fruits and other plant derived products have gained considerable attention as they can reduce the risk of several cancer types. Lupeol, a triterpene, present in many fruits and medicinal plants, has been shown to possess many pharmacological properties including anti-cancer effect in both in vitro and in vivo assay systems. In the present study, apoptosis inducing effects of lupeol were studied in human epidermoid carcinoma A431 cells. Cell cycle analysis showed that lupeol treatment induces apoptosis (14-37%) in a dose-dependent manner as evident by an increased sub G(1) cell population. The RT-PCR and Western blot analysis showed that lupeol-induced apoptosis was associated with caspase dependent mitochondrial cell death pathway through activation of Bax, caspases, Apaf1, decrease in Bcl-2 expression and subsequent cleavage of PARP. Lupeol treatment also inhibited Akt/PKB signaling pathway by inhibition of Bad (Ser136) phosphorylation and 14-3-3 expression. In addition, lupeol treatment inhibited cell survival by inactivation of NFkappaB through upregulation of its inhibitor Ikappabetaalpha. The Caspase mediated apoptosis was noticed by decrease in lupeol induced apoptosis by Caspase inhibitors as well as increase in reactive oxygen species generation and loss of mitochondrial membrane potential. These results suggest that lupeol could be an effective anti-cancer agent and merits further investigation.

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Ethanol Exposure Suppresses Survival Kinases Activation in Adult Rat Testes

Cited by 8 publications

References 19 publications

The Seminal Plasma MDA Level as Bio-Marker for Oxidative Stress and Their Impact on Seminogram in Primary Infertile Males, Erbil-Kurdistan Region, Iraq

The Seminal Plasma MDA Level as Bio-Marker for Oxidative Stress and Their Impact on Seminogram in Primary Infertile Males, Erbil-Kurdistan Region, Iraq

Testicular development of male mice offsprings exposed to acrylamide and alcohol during the gestation and lactation period

Induction of apoptosis by lupeol in human epidermoid carcinoma A431 cells through regulation of mitochondrial, Akt/PKB and NF-kappaB signaling pathways

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