Ethanol extract of Gynura bicolor (GB) protects against UVB-induced photodamage of skin by inhibiting P53-mediated Bcl-2/BAX/Caspase-3 apoptosis pathway

Li, Lei; Han, Xiaofeng; Gao, Yanni; Diao, Qingchun; Xiao, Yujuan

doi:10.1007/s00403-019-01977-y

Cited by 14 publications

(10 citation statements)

References 12 publications

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“…Many apoptosis inhibitors can act as antioxidants. [6][7][8][9][10] And if DNA damage is not repaired, it may F I G U R E 1 The primary mechanism of keratinocytes PCD under UV irradiation.UV irradiation induces different forms of PCD of keratinocytes, including apoptosis, pyroptosis, necroptosis and autophagy. The black arrows illustrate the mechanisms of apoptosis, including intrinsic pathway (A) and extrinsic pathway (B).…”

Section: Keratinocyte Apoptosis During Skin Photodamagementioning

confidence: 99%

“…A recent study showed that an ethanol extract of Gynura bicolour (GB) had a protective effect against photodamage by downregulating the p53-mediated Bcl-2/Bax/ Caspase-3 apoptosis pathway in HaCaT keratinocytes. 7 In contrast, zinc oxide nanoparticles synthesized from Allium cepa (ZnO-NPs) could exacerbate HaCaT cell apoptosis by inducing the expression of Bax and a corresponding decrease in Bcl2, which indicated that ZnO-NPs may be a promising candidate for the treatment of UVrelated skin diseases. 14 Fas belongs to the TNFR family and is involved in initiating the extrinsic death pathway in keratinocytes.…”

Section: Keratinocyte Apoptosis During Skin Photodamagementioning

confidence: 99%

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Research progress of keratinocyte‐programmed cell death in UV‐induced Skin photodamage

Tang

Tong

Huang

et al. 2021

Photoderm Photoimm Photomed

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Skin photodamage includes sunburn, phototoxic dermatitis, photosensitive dermatitis and photoaging. These common skin diseases that affect appearance are caused by ultraviolet radiation. The ultraviolet rays that reach the Earth are mainly divided into longwave ultraviolet rays (ultraviolet radiation A, UVA, 320 ~ 400 nm) and medium-wave ultraviolet rays (ultraviolet radiation B, UVB, 290 ~ 320 nm). UVA accounts for 95% of the ultraviolet radiation that reaches the Earth's surface 1 and leads to skin ageing and wrinkling because of cellular DNA damage by reactive oxygen species (ROS) production. 2,3 UVB is a major cause of skin reddening and sunburn through direct DNA damage. 3 Therefore, both types of UV radiation are chief causes of skin damage and can induce skin inflammation, ageing and eventually skin cancer. Human keratinocytes, as the outermost barrier of the skin, are the main target cells of UV radiation. Studies have shown that UV irradiation can induce different forms of programmed cell death (PCD) in keratinocytes, including apoptosis, pyroptosis, necroptosis and autophagy (Figure 1). In this review, we will briefly introduce the various types of PCD and their roles in UV-induced skin photodamage. | AP OP TOS IS | Introduction to apoptosisApoptosis is type of cell death in which the cell membrane remains intact. Apoptosis essentially does not induce inflammatory reactions. Initiator caspases (mainly caspase-2, −8, −9 and −10) and executioner caspases (such as caspase-3, −6 and −7) participate in the process of apoptosis. Apoptosis controls the development and homeostasis of multicellular organisms, and the distinct morphological characteristics of apoptosis are cell shrinkage and pyknosis,

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Section: Keratinocyte Apoptosis During Skin Photodamagementioning

confidence: 99%

Section: Keratinocyte Apoptosis During Skin Photodamagementioning

confidence: 99%

Research progress of keratinocyte‐programmed cell death in UV‐induced Skin photodamage

Tang

Tong

Huang

et al. 2021

Photoderm Photoimm Photomed

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“…In addition, cryptotanshinone (CTS), a component of Salvia miltiorrhiza Bunge, can ameliorate ischemia/reperfusion injury in HK-2 cells, human proximal tubular cells, in vitro partly by regulating Bax, Bcl-2, and caspase-3 expression to decrease apoptosis [51]. Li et al (2019) showed that an ethanol extract of G. bicolor can protect against UVB-induced apoptosis of skin HaCaT cells by reducing Bax and caspase-3 mRNA expression and increasing Bcl-2 mRNA expression [24]. In the present study, GBAE signi cantly decreased the apoptosis of kidney epithelial NRK-52E cells induced by t-BHP by decreasing the level of ROS and the expression of Bax, and PARP and increasing the level of Bcl-2.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies showed that aqueous G. bicolor extracts can attenuate H 2 O 2 -induced injury in arti cial rat neurons (PC-12 cells) by decreasing ROS, IL-1β, IL-6, and TNF-α levels and increasing GSH levels and GSH peroxidase activity [5]. Ethanol extracts of G. bicolor also protect against UVB-induced photodamage in human skin keratinocytes (HaCaT cells) by inhibiting apoptosis [24]. Whether G. bicolor can protect the kidney from damage is unknown.…”

Section: Introductionmentioning

confidence: 97%

Protective Effect of Gynura Bicolor Alcohol Extracts on Rat Kidney Epithelial NRK-52E Cells Induced by Tert-Butyl Hydroperoxide

Hsieh¹,

Wu²,

Wang³

et al. 2020

Preprint

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Background: Gynura bicolor is a common vegetable in the Asia and has many physiological effects. We aimed to investigate the renoprotective effects of G. bicolor alcohol extracts (GBAEs). Methods: Rat kidney epithelial NRK-52E cell culture and kidney damage induction by tert-butyl hydroperoxide (t-BHP) were used as experimental models. In this study, the antioxidative and anti-inflammatory activities, the levels of apoptosis and apoptosis-related molecules were analyzed in NRK-52E cells following t-BHP-induced injury. Results: The results showed the levels of thiobarbituric acid reactive substances (TBARS) and reactive oxygen species (ROS) decreased by levels by 61-71% and 41-59%, respectively, in NRK-52E cells treated with 0.5, 1, or 5 mg/ml GBAE combined with t-BHP compared with t-BHP-induced cells (p<0.05). The GSH levels and GSH-to-GSSG ratio were increased in NRK-52E cells treated with 0.5, 1, and 5 mg/ml GBAE combined with t-BHP compared to t-BHT-induced cells (p<0.05). The nitrite (NO), prostaglandin E2 (PGE2), IL-6, and TNF-α levels of NRK-52E cells treated with 0.5, 1, and 5 mg/ml GBAE combined with t-BHP were decreased compared with t-BHP-induced cells (p<0.05). In addition, Bcl-2 levels were increased after treatment with 5 mg/ml GBAE combined with t-BHP, and the levels of Bax and poly (ADP-ribose) polymerase (PARP) and the apoptosis percentage were decreased after treatment with 1 or 5 mg/ml GBAE combined with t-BHP induction compared to after t-BHP induction alone (p<0.05). Conclusions: These data showed that 5 mg/ml GBAE can protect against kidney cell damage induced by t-BHP. The mechanism involves reducing cell damage and apoptosis through decreasing oxidative stress and inflammation.

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“… 2019 ), protective of skin’s photodamage (Li et al. 2020 ), and decrease serum cholesterol levels (Hsieh et al. 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Ethanol extract of Gynura bicolour reduces atherosclerosis risk by enhancing antioxidant capacity and reducing adhesion molecule levels

Hsieh

Wang

Huang

et al. 2021

Pharmaceutical Biology

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Context Gynura bicolour (Roxb. and Willd.) DC (Asteraceae) leaf is a common vegetable. Ethanol extracts of fresh G. bicolour leaves (GBEE) have several physiological effects, but studies on atherosclerosis are limited. Objective We investigated the oxidant scavenging ability and vascular adhesion molecule expression of these extracts. Materials and methods The antioxidant effects of 0.05–0.4 mg/mL GBEE were analyzed in vitro . Intracellular antioxidant capacity and adhesion molecule levels were detected in EA.hy926 cells pre-treated with 10–100 μg/mL GBEE for 8 h, then TNF-α for 3 h. The antioxidant capacity of red blood cells and the adhesion molecule levels in the thoracic aorta were detected in high-fat diet (HFD)-fed Sprague–Dawley rats treated with GBEE for 12 weeks. Results The in vitro EC 50 values of GBEE based on its DPPH radical-scavenging ability, reducing power, and ferrous ion-chelating ability were 0.20, 3.21 and 0.49 mg/mL, respectively. In TNF-α-treated EA.hy926 cells, the thiobarbituric acid-reactive substance levels were decreased after 10, 50, or 100 μg/mL GBEE treatments (IC 50 : 19.1 mg/mL). When HFD-fed rats were co-treated with GBEE, the GBEE-H group exhibited 25% higher glutathione levels than the HFD group ( p < 0.05). E-selectin, intercellular adhesion molecule-1, and vascular cell adhesion protein-1 levels were decreased in TNF-α-treated EA.hy926 cells after GBEE treatment (by approximately 11–73%; p < 0.05), and the above three adhesion molecules levels were decreased in HFD-fed rats with combined GBEE treatment (by approximately 30–77%; p < 0.05). Conclusions GBEE can protect the vascular endothelium by reducing adhesion molecule expression and regulating antioxidants. It may have the potential to prevent atherosclerosis.

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Ethanol extract of Gynura bicolor (GB) protects against UVB-induced photodamage of skin by inhibiting P53-mediated Bcl-2/BAX/Caspase-3 apoptosis pathway

Cited by 14 publications

References 12 publications

Research progress of keratinocyte‐programmed cell death in UV‐induced Skin photodamage

Research progress of keratinocyte‐programmed cell death in UV‐induced Skin photodamage

Protective Effect of Gynura Bicolor Alcohol Extracts on Rat Kidney Epithelial NRK-52E Cells Induced by Tert-Butyl Hydroperoxide

Ethanol extract of Gynura bicolour reduces atherosclerosis risk by enhancing antioxidant capacity and reducing adhesion molecule levels

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Ethanol extract of Gynura bicolor (GB) protects against UVB-induced photodamage of skin by inhibiting P53-mediated Bcl-2/BAX/Caspase-3 apoptosis pathway

Cited by 14 publications

References 12 publications

Research progress of keratinocyte‐programmed cell death in UV‐induced Skin photodamage

Research progress of keratinocyte‐programmed cell death in UV‐induced Skin photodamage

Protective Effect of Gynura Bicolor Alcohol Extracts on Rat Kidney Epithelial&nbsp;NRK-52E Cells Induced by Tert-Butyl Hydroperoxide

Ethanol extract of Gynura bicolour reduces atherosclerosis risk by enhancing antioxidant capacity and reducing adhesion molecule levels

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Protective Effect of Gynura Bicolor Alcohol Extracts on Rat Kidney Epithelial NRK-52E Cells Induced by Tert-Butyl Hydroperoxide