Ethanol extracts of Panax notoginseng increase lifespan and protect against oxidative stress in Caenorhabditis elegans via the insulin/IGF-1 signaling pathway

Jin, Shuguang; Li, Dang-Qing; Lu, Shan; Han, Lintao; Liu, Dahui; Huang, Zhuang; Huang, Bisheng; Cao, Yanjun

doi:10.1016/j.jff.2019.04.031

Cited by 17 publications

(14 citation statements)

References 17 publications

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“…Indeed, several TCMs have been reported as important sources of active compounds that decrease the accumulation of autofluorescent material in C. elegans, but most are hard to compare to our work. For instance, a Panax notoginseng preparation astonishingly reduced the autofluorescence by roughly one half ( Jin et al, 2019 ). However, the concentrations used were more than 25 times higher compared to the current study.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Healthspan in Caenorhabditis elegans Treated With Extracts From the Traditional Chinese Medicine Plants Cuscuta chinensis Lam. and Eucommia ulmoides Oliv.

Sayed

Siems²,

Schmitz‐Linneweber

et al. 2021

Front. Pharmacol.

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To uncover potential anti-aging capacities of Traditional Chinese Medicine (TCM), the nematode Caenorhabditis elegans was used to investigate the effects of Eucommia ulmoides and Cuscuta chinensis extracts, selected by screening seven TCM extracts, on different healthspan parameters. Nematodes exposed to E. ulmoides and C. chinensis extracts, starting at the young adult stage, exhibited prolonged lifespan and increased survival after heat stress as well as upon exposure to the pathogenic bacterium Photorhabdus luminescens, whereby the survival benefits were monitored after stress initiation at different adult stages. However, only C. chinensis had the ability to enhance physical fitness: the swimming behavior and the pharyngeal pumping rate of C. elegans were improved at day 7 and especially at day 12 of adulthood. Finally, monitoring the red fluorescence of aged worms revealed that only C. chinensis extracts caused suppression of intestinal autofluorescence, a known marker of aging. The results underline the different modes of action of the tested plants extracts. E. ulmoides improved specifically the physiological fitness by increasing the survival probability of C. elegans after stress, while C. chinensis seems to be an overall healthspan enhancer, reflected in the suppressed autofluorescence, with beneficial effects on physical as well as physiological fitness. The C. chinensis effects may be hormetic: this is supported by increased gene expression of hsp-16.1 and by trend, also of hsp-12.6.

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Section: Discussionmentioning

confidence: 99%

Enhanced Healthspan in Caenorhabditis elegans Treated With Extracts From the Traditional Chinese Medicine Plants Cuscuta chinensis Lam. and Eucommia ulmoides Oliv.

Sayed

Siems²,

Schmitz‐Linneweber

et al. 2021

Front. Pharmacol.

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“…The lifespan assay was based on previously published protocol [46] . Briefly, in this study, 0.2 % DMSO was used as blank control, and RES (100 μM) as positive control.…”

Section: Methodsmentioning

confidence: 99%

Phytochemical Composition and Anti‐Aging Activity of Butanol Extract of Hedyotis diffusa in Caenorhabditis elegans

Liu

Dan-qing

et al. 2022

Chemistry & Biodiversity

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Hedyotis diffusa Willd. (H. diffusa), a kind of traditional Chinese medicine, has been evaluated to potential display antioxidant and anti‐aging effects in vitro experiments. In this work, we investigated the effects on lifespan and stress resistance of the butanol extract from H. diffusa (NHD) in vivo using a Caenorhabditis elegans (C. elegans) model. The phytochemicals of NHD were identified by UPLC‐ESI‐qTOF‐MS/MS method. NHD‐treated wild‐type N2 worms showed an increase in survival time under both normal and stress conditions. Meanwhile, NHD promoted the healthspan of nematodes by stimulating growth and development, reducing the deposition of age pigment, increasing the activities of superoxide dismutase (SOD) and glutathione peroxidase dismutase (GSH‐Px), and decreasing the level of ROS without impairing fertility. Moreover, the upregulating of the expression of daf‐16, gst‐4, sod‐3, hsp12.6 genes and the downregulating of the expression of daf‐2 were involved in the NHD‐mediated lifespan extension. Finally, the increasing of the expression of GST‐4::GFP in CL2166 transgenic nematodes and the life‐span‐extending activity of NHD was completely abolished in daf‐2 and daf‐16 mutants further revealed that the potential roles for these genes in NHD‐induced longevity in C. elegans. Collectively, our findings suggest that NHD may have an active effect in healthy aging and age‐related diseases.

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“…The nematodes were prepared as described above and then transferred to OP50 plates or LAB plates and cultured at 20 °C for 10 days. The heat stress assay and the oxidative stress assay methods were as previously reported, 16 and the number of live nematodes was counted every hour until all the nematodes had died. All measurements were repeated three times, with at least 10 worms in each group.…”

Section: Fertility Measurementmentioning

confidence: 99%

“…12,13 The literature indicates that C. elegans has been widely used as an efficient model to study the effects on ageing of functional foods with antioxidant properties. [14][15][16] In addition, there is a growing need for fast and reliable toxicity assays to evaluate potential threats to food safety, and C. elegans can be used as a model to test the safety of materials. 17 To our knowledge, however, few studies have been published on evaluating anti-ageing effect of LAB strains isolated from traditional fermented yak milk by using C. elegans as a model.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant properties of lactic acid bacteria isolated from traditional fermented yak milk and their probiotic effects on the oxidative senescence ofCaenorhabditis elegans

Li’e

Huang

et al. 2022

Food Funct.

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Ethanol extracts of Panax notoginseng increase lifespan and protect against oxidative stress in Caenorhabditis elegans via the insulin/IGF-1 signaling pathway

Cited by 17 publications

References 17 publications

Enhanced Healthspan in Caenorhabditis elegans Treated With Extracts From the Traditional Chinese Medicine Plants Cuscuta chinensis Lam. and Eucommia ulmoides Oliv.

Enhanced Healthspan in Caenorhabditis elegans Treated With Extracts From the Traditional Chinese Medicine Plants Cuscuta chinensis Lam. and Eucommia ulmoides Oliv.

Phytochemical Composition and Anti‐Aging Activity of Butanol Extract of Hedyotis diffusa in Caenorhabditis elegans

Antioxidant properties of lactic acid bacteria isolated from traditional fermented yak milk and their probiotic effects on the oxidative senescence ofCaenorhabditis elegans

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