Ethanol increases ADP-ribosylation of histones in rat hepatocyte nuclei

Akinshola, B. Emmanuel; Potter, James J.; Mezey, Esteban

doi:10.1016/0741-8329(93)90098-9

Cited by 2 publications

(1 citation statement)

References 13 publications

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“…In our previous studies of investigating various concentrations of ethanol on hepatocytes, we observed that 100 mM is suitable as a hepatic injury model concentrations as no cytotoxicity was observed at 50 mM and severe cytotoxicity was induced at 200 mM (Takahashi et al, 2012). This concentration to induce hepatotoxicity has also been used in other studies, including our previous ones, and hence the same choice in this study (Akinshola et al, 1993; Shao et al, 2021; Kojima‐Yuasa et al, 2016; Wu & Cedetbaum, 1996; Yamashita et al, 2015).…”

Section: Methodsmentioning

confidence: 94%

Black carrot extract protects against hepatic injury through epigenetic modifications

Kitano

Norikura

Matsui–Yuasa

et al. 2022

Journal of Food Biochemistry

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We studied the epigenetic regulation of how black carrot extract (BCE) protects against ethanol‐induced hepatic damage. We have shown that the butanol‐extracted fraction of BCE (BCE‐BuOH) increased intracellular cyclic adenosine monophosphate (cAMP) levels by suppressing the expression of phosphodiesterase 4b (PDE4b); however, the detailed mechanism remains to be elucidated. We focused on changes in histone modifications involved in the suppression of pde4 expression. The methylation level of histone H3 lysine 9 (H3K9), which regulates gene expression of PDE4b, decreased after treatment with 100 mM ethanol but was significantly increased by treatment with 400 μg/ml BCE‐BuOH. In contrast, ethanol induced an increase in H3K9 acetylation. However, treatment with BCE‐BuOH inhibited the increase in acetylation through an increase in Sirtuin 1 (Sirt1), a histone deacetylase. Furthermore, BCE‐BuOH treatment increased the level of methionine adenosyltransferase (MAT) 2a mRNA and increased intracellular S‐adenosylmethionine. The present results indicate that BCE‐BuOH is useful for protection against alcohol‐induced hepatic injury. Practical applications We have reported that black carrot extract (BCE) suppressed liver steatosis and liver fibrosis on a rat alcoholic liver disease model. The results from this study have shown that BCE regulated the alcoholic‐induced hepatic injury at the level of epigenetic modifications. These results suggested that BCE is useful for protection against alcoholic‐induced hepatic injury.

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