Ethanol-induced apoptosis in mouse liver: Cytochrome c-activated caspase 3 pathway

Zhou, Zhanxiang; Zhong, Xiaoyun; Kang, Y. James

doi:10.1016/s0016-5085(00)85993-4

Cited by 55 publications

(99 citation statements)

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“…Aroor and Baker (1997) reported that the apoptotic cell number was significantly elevated in the human HL-60 promyeolocytic cell line after ethanol treatment. After a single day of binge ethanol treatment, Zhou et al (2001) detected apoptotic cells in the liver, and Fas/Fas ligand systemmediated caspase-3 activation was postulated to play a central role in this apoptotic pathway, but mitochondrial cytochrome c release was also involved. Low-concentration ethanol treatment can also lead to apoptosis, probably through Fas receptor activation in a human hepatocellular carcinoma cell line (Castaneda and Kinne 2001;Kai et al 2010).…”

Section: The Effects Of Alcohol On Cellular Physiologymentioning

confidence: 99%

Alcohol stress, membranes, and chaperones

Tóth

Vı́gh

Sántha

2014

Cell Stress and Chaperones

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Ethanol, which affects all body organs, exerts a number of cytotoxic effects, most of them independent of cell type. Ethanol treatment leads to increased membrane fluidity and to changes in membrane protein composition. It can also interact directly with membrane proteins, causing conformational changes and thereby influencing their function. The cytotoxic action may include an increased level of oxidative stress. Heat shock protein molecular chaperones are ubiquitously expressed evolutionarily conserved proteins which serve as critical regulators of cellular homeostasis. Heat shock proteins can be induced by various forms of stresses such as elevated temperature, alcohol treatment, or ischemia, and they are also upregulated in certain pathological conditions. As heat shock and ethanol stress provoke similar responses, it is likely that heat shock protein activation also has a role in the protection of membranes and other cellular components during alcohol stress.

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Section: The Effects Of Alcohol On Cellular Physiologymentioning

confidence: 99%

Alcohol stress, membranes, and chaperones

Tóth

Vı́gh

Sántha

2014

Cell Stress and Chaperones

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“…Potential mechanisms of acute ethanolinduced liver apoptosis include increased cytokine activity, Fas ligand (FasL) expression, and/or oxidative stress (Kurose et al, 1997, Neuman et al, 2001. Ethanol-induced liver apoptosis involves the activation of cysteine proteases or caspases (Deaciuc et al, 1999, Zhou et al, 2001. Endonucleases and DNA fragmentation factors are activated during apoptosis, resulting in degradation of chromatin DNA into internucleosomal units (Cohen andDuke, 1984, Liu et al, 1997).…”

Section: Effect Of Alcohol Intakementioning

confidence: 99%

The protective efficacy of vitamins (C and E), selenium and silymarin supplements against alcohol toxicity

Shalan

Ali²,

Shalan

2010

World rabbit sci.

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ABSTRACT:This study aimed at investigating the efficacy of vitamins (C and E), selenium and silymarin (an antioxidant complex from Silybum marianum) supplementation in reducing toxic effects of ethanol on liver weight and some blood parameters. Sixty male rabbits, individually housed in steel cages, were randomly divided into three groups. The first was a control group, the second received balanced diet and daily 20% (v/ v) ethyl alcohol in their drinking water, the third received the same diet and 20% (v/v) ethanol in their drinking water and treated with vitamin C (1 mg/100 g body weight, BW), vitamin E (1 mg/100 g BW), selenium (0.01 mg/ 100 g BW) and silymarin (1 mg/100 g body weight) by gastric tube daily. Five animals per group were slaughtered every two weeks and liver and blood samples were taken after 2, 4, 6 and 8 weeks of treatment. Ethanol decreased body weight of rabbits and induced hepatomegally and apoptotic DNA fragmentation in hepatocytes. Chronic alcohol consumption induced significant increases in serum glucose, triglycerides and cholesterol levels whereas serum total protein content decreased. Significant increases in serum ALT, AST, ALP and LDH activities were observed in ethanol-treated rabbits. The treatment of alcohol-abused animals with vitamins (C and E), selenium and silymarin enhanced significant improvement in the biochemical, physiological and molecular aspects indicating their protective effects against alcohol toxicity.

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“…Das et al (2011) have shown that long-term EtOH consumption increases caspase-3 activity in rat liver tissue. Zhou et al (2001) have also found that exposure to ethanol caused an increase in caspase-3 activation in rat liver tissue. Although not statistically significant, the caspase-3 activity in Group II, to which alcohol was administered, was higher than that in the other groups.…”

Section: Discussionmentioning

confidence: 76%

“…This increase was not significant. In various clinical and experimental studies, it was reported that alcohol-induced serum ALT levels were elevated during alcoholic liver disease (Kim et al, 2009;Pari & Suresh, 2008;Zhou et al, 2001). For the AST levels, there was a decline in Group II (EtOH control group), while there was an increase in the PS groups.…”

Section: Discussionmentioning

confidence: 95%

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Consumption ofCoprinus comatuspolysaccharide extract causes recovery of alcoholic liver damage in rats

Ozalp

Canbek

Yamaç

et al. 2014

Pharmaceutical Biology

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Context: Excess use of alcohol is known to be associated with liver diseases such as fatty liver, alcoholic hepatitis, and cirrhosis. Various practices may be applied to prevent or treat the damage caused by chronic alcoholism. Coprinus comatus (O.F. Mü ll.) Pers. (Agaricaceae) is a macrofungus that has been reported to aid the recovery of murine livers damaged by benzopyrene. Objective: In this study, the possible therapeutic effects of three different doses (50, 100, and 150 mg/kg) of C. comatus polysaccharide (PS) extract were studied in rats subjected to an alcoholic diet. The histological and biochemical results were compared between the control and experimental groups. Materials and methods: Modified Lieber-Decarli's calorie-adjusted liquid alcohol diet was given orally for 60 d. In addition to histopathology, alanine transaminase (ALT), aspartate transaminase (AST), mitochondrial membrane integrity, total cytochrome-c oxidase activity (TotalStCox), total mitochondrial cytochrome-c oxidase activity (TotalMtStCox), and caspase-3 values were used as liver parameters, and liver sections from all experimental groups were examined by electron microscopy. Results: Using histopathological assessment, it was observed that there was a decline in liver hepatocyte vacuolization in the treatment group fed 50 mg PS/kg. The TotalStCox and TotalMtStCox values of this group differed from the EtOH control group (p50.05). Discussion and conclusion: Daily administration of 50 mg/kg of C. comatus PS extract considerably reduced the negative effects of alcohol on liver structure and function.

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Ethanol-induced apoptosis in mouse liver: Cytochrome c-activated caspase 3 pathway

Cited by 55 publications

References 0 publications

Alcohol stress, membranes, and chaperones

Alcohol stress, membranes, and chaperones

The protective efficacy of vitamins (C and E), selenium and silymarin supplements against alcohol toxicity

Consumption ofCoprinus comatuspolysaccharide extract causes recovery of alcoholic liver damage in rats

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