Ethanol-induced Germ Tube Formation in Candida albicans

Pollack, Jordan H.; Hashimoto, Tadayo

doi:10.1099/00221287-131-12-3303

Cited by 13 publications

(12 citation statements)

References 28 publications

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“…However, several workers have reported a limited degree of anaerobic growth [28,48,65,87,94]. While examining the growth of single isolates of several Candida species in anaerobic gas jars, Webster and Odds found that except C. guillier-mondii and C. parapsilosis, all the isolates, including C. albicans, could grow to some extent [121].…”

Section: Growth Under Limited Aerobic Conditionsmentioning

confidence: 98%

Growth and Respiration Characteristics of Candida albicans

Anand

Prasad

1991

Candida Albicans

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Section: Growth Under Limited Aerobic Conditionsmentioning

confidence: 98%

Growth and Respiration Characteristics of Candida albicans

Anand

Prasad

1991

Candida Albicans

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“…Should the pH drop below 6, the cells revert to growing as a budding yeast. Using ethanol as an inducer of germ-tube formation (Pollack & Hashimoto, 1985), we have shown that germtubes could form even when the pH was as low as 3.0. This led us to look for an explanation for the differences between our findings and those of Soll and co-workers (Mitchell & Soll, 1979;Buffo et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

The Role of Glucose in the pH Regulation of Germ-tube Formation in Candida albicans

Pollack¹,

Hashimoto²

1987

Microbiology

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It has been reported that Candida albicans can form germ-tubes only in the narrow pH range of 6-8, and that by changing only the pH one can regulate germ-tube formation. We found that the pH minimum for germ-tube formation could be dramatically lowered by eliminating the glucose present in many induction solutions. Lee's medium lacking glucose, ethanol, N-acetyl-D-glucosamine, and proline induced germ-tubes at pH values as low as 3 under most conditions. The presence of as little as 1 mM-glucose in these induction solutions was sufficient to cause the cells to grow either as yeasts with multiple buds or as pseudohyphae when the pH was 3.7. However, when C. albicans was grown in any of the above induction solutions (with the exception of ethanol), containing 200 mM-glucose buffered at pH 5.8, not only were germ-tubes formed, but their rate of formation and length were also increased. Preincubation of the cells in a solution buffered at pH 3.7 and containing 200 mM-glucose, before exposure to induction solutions lacking glucose at pH 3.7 or at pH 5.8, did not inhibit germ-tube formation. Likewise, addition of glucose after 45 min exposure to an induction solution was without effect. Theophylline and dibutyryl cAMP did not counteract the action of glucose. Other sugars which suppressed germ-tube formation at low pH were fructose, galactose, mannose, xylose, gluconic acid and the nonmetabolizable sugar 3-O-methylglucose. These results indicate that pH does not directly regulate dimorphism in C. albicans, and that glucose or its metabolites may play an important role.

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“…Australian Dental Journal 1998;43:(1): [45][46][47][48][49][50] C a n d i d a -a s s o c i a ted denture stoma t i t i s . Aetiology and m a n a g e m e n t : A r e v i e w .…”

Section: Introductionmentioning

confidence: 99%

Candida‐associated denture stomatitis. Aetiology and management: A review: Part1. Factors influencing distribution of candida species in the oral cavity

Webb

Thomas

Willcox

et al. 1998

Australian Dental Journal

205

162

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Candida species are yeasts and within the oral cavity, Candida albicans is the most frequently isolated. There is clear evidence that C. albicans adheres to oral surfaces including acrylic dentures and mucosa. The mechanisms of attachment differ, with candidal adhesion to inert surfaces under the control of hydrophobic and electrostatic forces and adhesion to mucosa dependent on a number of complex ligand-recognition systems. Other factors within the oral environment such as saliva, pH, bacteria and hyphal formation have been shown to influence adhesion of candida species to surfaces in the mouth.

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Ethanol-induced Germ Tube Formation in Candida albicans

Cited by 13 publications

References 28 publications

Growth and Respiration Characteristics of Candida albicans

Growth and Respiration Characteristics of Candida albicans

The Role of Glucose in the pH Regulation of Germ-tube Formation in Candida albicans

Candida‐associated denture stomatitis. Aetiology and management: A review: Part1. Factors influencing distribution of candida species in the oral cavity

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