Ethanol inhibits L-arginine uptake and enhances NO formation in human placenta

Acevedo, C.G.; Carrasco, Gonzalo; Burotto, Mauricio; Rojas, Susana; Bravo, Iván

doi:10.1016/s0024-3205(01)01070-0

Cited by 48 publications

(30 citation statements)

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“…All uptake measurements were made in triplicate. Fructose, L-alanine, or L-arginine uptake experiments were conducted as described by the method of Corpe et al [31], Nishida et al [32], or Acevedo et al [33], respectively.…”

Section: Uptake Experiments [mentioning

confidence: 99%

PKC and MAPKs Pathways Mediate EGF-induced Stimulation of 2-Deoxyglucose Uptake in Mouse Embryonic Stem Cells

Heo

Han

2006

Cell Physiol Biochem

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It has been reported that epidermal growth factor (EGF) and EGF receptor were highly expressed in embryo, suggesting that the EGF system is related to early embryo development in an autocrine and/or paracrine manner. Glucose becomes the preimplantation exogenous energy substrate and enters the blastocyst via glucose transporters. Thus, the effect of EGF on [3H]-2-deoxyglucose (2-DG) uptake and its related signaling pathways were examined in mouse embryonic stem (ES) cells. EGF significantly increased 2-DG uptake in time- and concentration- dependent manner (>12 hr, >10 ng/ ml) and increased mRNA and protein level of glucose transporter 1 (GLUT1) compared to control, respectively. Actinomycin D and cycloheximide completely blocked the effect of EGF on 2-DG uptake. EGF-induced increase of 2-DG uptake was blocked by AG1478 (EGF receptor tyrosine kinase blocker), genistein or herbimycin (tyrosine kinase inhibitors). In addition, EGF effect was blocked by neomycin and U 73122 [phospholipase C (PLC) inhibitors] as well as staurosporine and bisindolylmaleimide I [protein kinase C (PKC) inhibitors]. EGF was also observed to increase inositol phosphates (IPs) formation and activate a PKC translocation from the cytosolic to membrane fraction, suggesting a role of PLC and PKC. SB 203580 [p38 mitogen activated protein kinase (MAPK) inhibitor] or PD 98059 (p44/42 MAPKs inhibitor) blocked EGF-induced increase of 2-DG uptake. EGF also increased phosphorylation of p38 MAPK and p44/42 MAPKs, which was blocked by genistein or bisindolylmaleimide I, respectively. In conclusion, EGF partially increased 2-DG uptake via PKC, p38 MAPK, and p44/42 MAPKs in mouse ES cells.

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Section: Uptake Experiments [mentioning

confidence: 99%

PKC and MAPKs Pathways Mediate EGF-induced Stimulation of 2-Deoxyglucose Uptake in Mouse Embryonic Stem Cells

Heo

Han

2006

Cell Physiol Biochem

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“…Some studies suggest that oxidative stress plays a role in the etiology of adverse reproductive events in both women and men [39]. Oxidative stress occurs from an imbalance between reactive oxygen species and antioxidant actions.…”

Section: Discussionmentioning

confidence: 99%

Biochemical Study of the Effects of Insulin Resistance on Sex Hormones in Men and Women Type-2 Diabetic Patients / Meisan-Iraq

Al–Fartosy

Mohammed²

2017

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Obesity, a case accompanied by increased insulin resistance, is associated with low follicle stimulating hormone, luteinizing hormone, total testosterone and progesterone and estradiol levels. As there is no scientific report on diabetes complications and its effects on type 2 diabetic patients in the province of Meisan (southern of Iraq), the present investigation was done to evaluate the levels of body mass index, insulin resistance and the levels of sex hormones in serum of both men and women type 2 diabetic patients. Data obtained in the present study, show a significant increase in insulin resistance level (4.24 ± 0.57 Vs. 1.36±0.23 and 4.31 ± 0.59 Vs. 1.18 ±0.13, P < 0.05) respectively, in men and women type 2 diabetic patients compared to control. Also, the present study show a significant decrease in follicle stimulating hormone level (7.24±1.14 Vs. 12.79 ±0.87 and 38.14 ± 4.44 Vs. 54.16 ± 3.64, P < 0.05), a significant decrease in luteinizing hormone level (5.32 ± 0.74 Vs. 7.54± 1.27 and 30.12 ± 4.20 Vs. 43.33±2.07, P < 0.05), a significant decrease in testosterone level (4.69 ± 0.92 Vs. 8.20 ± 2.2 and 0.57 ± 0.11 Vs. 0.69± 0.21, P < 0.01), a significant decrease in progesterone level (0.25 ±0.03 Vs. 0.42 ± 0.10 and 0.30 ± 0.04 Vs. 0.46± 0.12, P < 0.05) and a significant decrease in estradiol level (25.04 ± 3.35 Vs. 33.80±8.4 and 34.33 ± 4.21 Vs. 41.42±5.64, P < 0.05) respectively, in men and women type 2 diabetic patients compared to control. In conclusion, the findings further demonstrated that insulin resistance plays an important role in the pathogenesis of many human diseases, such as diabetes, obesity, and reduction of fertility. Therefore, the associated of obesity and increased insulin resistance is accompanied by low levels of follicle stimulating hormone, luteinizing hormone, testosterone, progesterone and estradiol in both men and women.

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“…A multiple logistic regression model was applied to estimate the age-and gender-adjusted ORs and their 95% CIs. Because tobacco smoke and ethanol play important roles in modulating eNOS activity [38][39][40], cigarette smoking and alcohol consumption (never and had ever) were included in the multiple logistic regression models.…”

Section: Discussionmentioning

confidence: 99%

“…Endothelial cell experimental studies demonstrated that both acute and chronic ethanol exposure may increase NOS3 gene transcription and increase NO production [38,39]. Alcohol consumption may thus overwhelm the genetic advantage of the T-786C and intron 4b/a substitutions.…”

Section: Discussionmentioning

confidence: 99%

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Association between Endothelial Nitric Oxide Synthase Polymorphisms and Risk of Metabolic Syndrome

et al. 2013

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Abstract. BACKGROUND:Previous studies inferring that the NOS3 gene was associated with the pathogenesis of metabolic syndrome (MetS) had inconsistent findings. We investigated the role of three NOS3 polymorphisms (T-786C, intron 4b/a, and G894T) in the risk of MetS using a hospital-based case-control study. METHODS:We recruited 339 MetS cases and 783 non-MetS controls at a central Taiwanese hospital. Information on sociodemographic and lifestyle factors was obtained using a self-administered questionnaire. Genotypes of NOS3 polymorphisms were compared between cases and controls. Effects of interactions between gene polymorphisms and smoking and between gene polymorphisms and drinking on the risk of MetS were also determined. RESULTS: The T-786C TC+CC genotype was significantly associated with a decreased risk of MetS (odds ratio (OR), 0.63; 95% confidence interval (CI), 0.43-0.91), compared to the T-786C TT genotype, according to a logistic regression analysis. This beneficial effect was much greater for those who had ever smoked cigarettes (OR, 0.47; 95% CI, 0.26-0.87) or those who had not consumed alcohol (OR, 0.45; 95% CI,). In addition, the intron 4b/a variant genotype was marginally associated with a reduced risk of MetS (OR, 0.68; 95% CI, 0.47-1.00), compared to the intron 4b/a bb genotype, particularly for never alcohol consumers (OR, 0.56; 95% CI,. In the haplotype analysis, there was a 53% decrease in the MetS risk among C4bG haplotype carriers (OR, 0.47; 95% CI, 0.25-0.90), compared to those with the most common T4bG haplotype. CONCLUSIONS: Our results suggest that the NOS3 T-786C and intron 4b/a polymorphisms may contribute to the risk of MetS. Further studies are needed to confirm the findings.

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Ethanol inhibits L-arginine uptake and enhances NO formation in human placenta

Cited by 48 publications

References 0 publications

PKC and MAPKs Pathways Mediate EGF-induced Stimulation of 2-Deoxyglucose Uptake in Mouse Embryonic Stem Cells

PKC and MAPKs Pathways Mediate EGF-induced Stimulation of 2-Deoxyglucose Uptake in Mouse Embryonic Stem Cells

Biochemical Study of the Effects of Insulin Resistance on Sex Hormones in Men and Women Type-2 Diabetic Patients / Meisan-Iraq

Association between Endothelial Nitric Oxide Synthase Polymorphisms and Risk of Metabolic Syndrome

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