Ethanol inhibits NMDA-stimulated neurite growth by sensory neurons in vitro

Dow, K; Rj, Riopelle

doi:10.1097/00001756-199010000-00007

Cited by 8 publications

(4 citation statements)

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“…The effect of both treatments can be reversed by the addition of glutamate or NMDA, respectively (Pearce et al, 1987;Rashid and Cambray-Deakin, 1992). Similar results have been observed in cultured chick embryo sensory neurons (Dow and Riopelle, 1990). Blockade of the NMDA receptor in the dendrites of tectal cells by DL-APV or MK-801 treatment has been shown to cause an approxinate 50% reduction in axonal arbor density in the tecta of three-eyed tadpoles (Cline et al, 1990).…”

Section: Discussionsupporting

confidence: 73%

“…This process seems to be based on Hebbian mechanisms, and the N-methyl-Daspartate (NMDA) receptor, given its characteristics (e.g., voltage dependence), plays a key role. Numerous studies show that manipulations of the NMDA receptor can alter axonal arborization (Pearce et al, 1987;Brewer and Cotman, 1989;Cline et al, 1990;Dow and Riopelle, 1990;Rashid and Cambray-Deakin, 1992;O'Rourke et al, 1994;Baird et al, 1996;Rajan et al, 1998;Lin and Constantine-Paton, 1998). Nonetheless, the molecular mechanisms under-lying the effect of NMDA receptor activity on axonal growth have barely been studied.…”

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confidence: 99%

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Activity-dependent regulation of axonal growth: Posttranscriptional control of the GAP-43 gene by the NMDA receptor in developing hippocampus

Cantallops

Routtenberg

1999

J. Neurobiol.

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Section: Discussionsupporting

confidence: 73%

mentioning

confidence: 99%

Activity-dependent regulation of axonal growth: Posttranscriptional control of the GAP-43 gene by the NMDA receptor in developing hippocampus

Cantallops

Routtenberg

1999

J. Neurobiol.

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“…However, ethanol treatment profoundly inhibited the insulin-mediated up-regulation of NTP expression, together with the insulin-stimulated neuritic growth and synaptophysin expression. Ethanol neurotoxicity leading to impaired neurite formation [46], inhibition of neuronal differentiation [73][74][75][76] and altered gene expression [45,47,77] has been described both in vivo and in vitro. Furthermore, in a previous study we demonstrated transient ethanol-mediated down-regulation of p36 (annexin II), another insulin-responsive gene in PNET2 cells [77].…”

Section: Discussionmentioning

confidence: 99%

Ethanol inhibits insulin receptor substrate-1 tyrosine phosphorylation and insulin-stimulated neuronal thread protein gene expression

Bhavani

Wands

et al. 1995

Biochemical Journal

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Neuronal thread proteins (NTPs) are molecules that accumulate in the brains of patients with Alzheimer's disease, and may play a key role in both normal and neurodegenerative neuritic sprouting. In this investigation we determined whether NTP expression is up-regulated by insulin, an important neurotrophic factor that stimulates differentiation-associated neurite outgrowth, and studied the effects of ethanol, a known inhibitor of growth factor receptor tyrosine phosphorylation, on NTP expression and insulin-mediated signal transduction cascade in neuronal [primitive neuroectodermal tumour cell line 2; (PNET2)] cells. PNET2 cells were treated with 50 m-units/ml insulin in the presence or absence of 100 mM ethanol for 0.2-96 h, and cell proliferation and expression of NTP molecules were investigated by metabolic labelling, immunoprecipitation and immunohistochemical staining. Insulin stimulation resulted in an immediate increase in the levels of three (38, 18 and 15 kDa) of five NTP species (the others were of 26 and 21 kDa), followed by a decline in expression within 120 min; however, studies performed up to 96 h of culture demonstrated up-regulation by insulin of all five NTP species. Ethanol either abolished or severely muted the short- and long-term insulin-mediated upregulation of NTP expression, and substantially reduced insulin-mediated neuronal differentiation. The effects of ethanol on NTP gene expression were associated with impaired insulin-mediated tyrosine phosphorylation of both the insulin receptor beta subunit and the insulin receptor substrate-1 (IRS-1), resulting in decreased association of phosphatidylinositol 3-kinase with IRS-1. The findings suggest that ethanol may inhibit NTP expression associated with central nervous system neuronal differentiation by uncoupling the IRS-1-mediated insulin signal transduction pathway.

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“…However, EtOH and DMSO have a wide range of properties, including effects on membrane transport, connective tissue, diuresis, vasodilation, nerve blockade (analgesia), and nerve stimuli (Jacob and Herschler, 1986;Dow and Riopelle, 1990;Malpas et al, 1990;Wolf et al, 1999). It has been reported that EtOH potentiates the thermal response of the IR (thermal) receptors of T. flavoviridis .…”

Section: Discussionmentioning

confidence: 99%

Snake Infrared Receptors Respond to Dimethylsulfoxide in the Blood Stream

et al. 2004

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1. We used extracellular recording of the infrared (IR)-sensitive trigeminal ganglion (TG) neurons (primary neurons) of a crotaline snake, Trimeresurus flavoviridis, which has very sensitive thermoreceptors, to examine changes in the IR response induced by dimethylsulfoxide (DMSO), in vivo. 2. The responses in the TG were recorded after each concentration of DMSO (1, 10, and 25%) was administered in the bloodstream. 3. At a constant temperature, DMSO dose-dependently potentiated the IR-triggered discharges of IR-sensitive TG neurons in this snake. 4. It is suggested that the increased IR response to DMSO is due to its chemical effect, or to an indirect effect via its vasoactive role in the thermoreceptors of IR-sensitive snakes.

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Ethanol inhibits NMDA-stimulated neurite growth by sensory neurons in vitro

Cited by 8 publications

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Activity-dependent regulation of axonal growth: Posttranscriptional control of the GAP-43 gene by the NMDA receptor in developing hippocampus

Activity-dependent regulation of axonal growth: Posttranscriptional control of the GAP-43 gene by the NMDA receptor in developing hippocampus

Ethanol inhibits insulin receptor substrate-1 tyrosine phosphorylation and insulin-stimulated neuronal thread protein gene expression

Snake Infrared Receptors Respond to Dimethylsulfoxide in the Blood Stream

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