Ethanol production from mahula (Madhuca latifolia L.) flowers using free and immobilized (in Luffa cylindrical L. sponge discs) cells of Zymomonas mobilis MTCC 92

Abstract

A novel immobilization method involving Zymomonas mobilis immobilized in a luffa (Luffa cylindrica L.) matrix for ethanol production from mahula (Madhuca latifolia L.) flowers was investigated. The dried spongy fruits of luffa, a cucurbitaceous crop available in abundance in tropical and sub-tropical countries, have been found to be a promising material for immobilizing microbial cells. In our study, the cells not only survived, but they were also active physiologically for four growth cycles, yielding ethanol at 251.1±0.012, 247.9±0.08, 243.4±0.042 and 240.71±0.033 g/kg flowers in the first, second, third, and fourth cycles, respectively, after 96 h fermentation. Ethanol production by the immobilized cells was 9.2% higher than that by free cells. The ethanol yield (Yp/s), volumetric substrate uptake (Qs), and final sugar to ethanol conversion rate (%) obtained with luffa-immobilized cells of Z. mobilis were 0.439±0.004 g/g, 0.994±0.009 g/l/h, and 87.8%, respectively, which were 7.06, 2.62, and 6.92% higher than that of free cells.

Bioethanol production from Madhuca latifolia L. flowers by a newly isolated strain of Pichia kudriavzevii

“…Madhuca latifolia commonly known as "mahua" belonging to the family Sapotaceae is a deciduous tree frequently distributed in the tropical rain forests of Asia and Australia. 1 The tree is present abundantly in Central and North Indian plains and forests of Chhattisgarh, Madhya Pradesh, and Orissa states; jointly covering nearly 80% of M. latifolia trees in India. Three to five million M. latifolia trees are there in Chhattisgarh and Madhya Pradesh states only.…”

Production of sorbitol by repeated batch fermentation using immobilized of Lactobacillus plantarum Strain (BAA-793) via Solid State Fermentation

“…Whole cell immobilization occurs to some extent in all microbe-based industrial processes as well, including water and wastewater treatment (Pilkingtonn et al, 1998;Ibrahim et al, 2013;Amenaghawon and Oiwoh, 2015). Before this, the immobilization of whole cells process and its application in the bioprocessing has been interesting for 30 years and it also has introduced in industrials (Behera et al, 2011). Besides that, immobilization also consists immobilizing cells of microorganism inside or on the surface of a carrier in a way that preserves their catalytic activity (Jack and Zajic, 2006).…”