Ethanol production from selected lignocellulosic hydrolysates by genome shuffled strains of Scheffersomyces stipitis

Bajwa, Paramjit K.; Phaenark, Chetsada; Grant, Nicola; Xiao, Zhang; Paice, Michael G.; Martin, Vincent J. J.; Trevors, J. T.; Lee, Hung

doi:10.1016/j.biortech.2011.08.027

Cited by 29 publications

(6 citation statements)

References 18 publications

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“…Bajwa et al . were able to achieve a 50% ethanol conversion efficiency in wood hydrolysates with two mutant strains of S. stipitis obtained by genome shuffling in a hardwood SSL [ 24 ]. Nevertheless, the operational conditions used by Bajwa et al .…”

Section: Discussionmentioning

confidence: 99%

Adaptation of Scheffersomyces stipitis to hardwood spent sulfite liquor by evolutionary engineering

Pereira

Nogué

Frazao

et al. 2015

Biotechnol Biofuels

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BackgroundHardwood spent sulfite liquor (HSSL) is a by-product of acid sulfite pulping process that is rich in xylose, a monosaccharide that can be fermented to ethanol by Scheffersomyces stipitis. However, HSSL also contains acetic acid and lignosulfonates that are inhibitory compounds of yeast growth. The main objective of this study was the use of an evolutionary engineering strategy to obtain variants of S. stipitis with increased tolerance to HSSL inhibitors while maintaining the ability to ferment xylose to ethanol.ResultsA continuous reactor with gradually increasing HSSL concentrations, from 20% to 60% (v/v), was operated for 382 generations. From the final obtained population (POP), a stable clone (C4) was isolated and characterized in 60% undetoxified HSSL. C4 isolate was then compared with both the parental strain (PAR) and POP. Both POP and C4 were able to grow in 60% undetoxified HSSL, with a higher capability to withstand HSSL inhibitors than PAR. Higher substrate uptake rates, 7% higher ethanol efficiency and improved ethanol yield were obtained using C4.ConclusionS. stipitis was successfully adapted to 60% (v/v) undetoxified eucalyptus HSSL. A stable isolate, C4, with an improved performance in undetoxified HSSL compared to PAR was successfully obtained from POP. Owing to its improved tolerance to inhibitors, C4 may represent a major advantage for the production of bioethanol using HSSL as substrate.

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Section: Discussionmentioning

confidence: 99%

Adaptation of Scheffersomyces stipitis to hardwood spent sulfite liquor by evolutionary engineering

Pereira

Nogué

Frazao

et al. 2015

Biotechnol Biofuels

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“…Yeasts able to convert xylose into ethanol have been described like as Scheffersomyces (Pichia) stipitis , S. (Candida) shehatae and Pachysolen tannophilus . 1 , 2 However the yield has not reached a satisfactory level for industrial applications On the other hand, pentose-assimilating yeasts can be used for obtainment of high aggregate value products. The pentose-assimilating capacity of yeasts Pseudozyma antarctica PYCC 5048 T , P. aphidis PYCC 5535 T and P. rugulosa PYCC 5537 T and production of a glycolipid biosurfactant was demonstrate 3 as well as the production of xylitol and arabitol by Debaryomyces hansenii from pentose.…”

Section: Introductionmentioning

confidence: 99%

The isolation of pentose-assimilating yeasts and their xylose fermentation potential

Martins

Bocchini

Bezzerra-Bussoli

et al. 2018

Brazilian Journal of Microbiology

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For the implementation of cellulosic ethanol technology, the maximum use of lignocellulosic materials is important to increase efficiency and to reduce costs. In this context, appropriate use of the pentose released by hemicellulose hydrolysis could improve de economic viability of this process. Since the Saccharomyces cerevisiae is unable to ferment the pentose, the search for pentose-fermenting microorganisms could be an alternative. In this work, the isolation of yeast strains from decaying vegetal materials, flowers, fruits and insects and their application for assimilation and alcoholic fermentation of xylose were carried out. From a total of 30 isolated strains, 12 were able to assimilate 30 g L−1 of xylose in 120 h. The strain Candida tropicalis S4 produced 6 g L−1 of ethanol from 56 g L−1 of xylose, while the strain C. tropicalis E2 produced 22 g L−1 of xylitol. The strains Candida oleophila G10.1 and Metschnikowia koreensis G18 consumed significant amount of xylose in aerobic cultivation releasing non-identified metabolites. The different materials in environment were source for pentose-assimilating yeast with variable metabolic profile.

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“…Ability to tolerate inhibitory compounds in lignocellulose hydrolysates could reduce the need for detoxification procedures, and this can decrease the overall production cost of ethanol. Adaptation is frequently substituted by random mutagenesis [11] or using genome shuffling via protoplast fusion of strains with different genotypes [9,10]. To improve ethanol tolerance and production, similar approaches of random mutagenesis and genome shuffling were combined and used [58].…”

Section: Scheffersomyces (Pichia) Stipitismentioning

confidence: 99%

Construction of advanced producers of first- and second-generation ethanol in Saccharomyces cerevisiae and selected species of non-conventional yeasts (Scheffersomyces stipitis, Ogataea polymorpha)

Ruchała

Kurylenko

Dmytruk

et al. 2020

Journal of Industrial Microbiology and Biotechnology

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This review summarizes progress in the construction of efficient yeast ethanol producers from glucose/sucrose and lignocellulose. Saccharomyces cerevisiae is the major industrial producer of first-generation ethanol. The different approaches to increase ethanol yield and productivity from glucose in S. cerevisiae are described. Construction of the producers of secondgeneration ethanol is described for S. cerevisiae, one of the best natural xylose fermenters, Scheffersomyces stipitis and the most thermotolerant yeast known Ogataea polymorpha. Each of these organisms has some advantages and drawbacks. S. cerevisiae is the primary industrial ethanol producer and is the most ethanol tolerant natural yeast known and, however, cannot metabolize xylose. S. stipitis can effectively ferment both glucose and xylose and, however, has low ethanol tolerance and requires oxygen for growth. O. polymorpha grows and ferments at high temperatures and, however, produces very low amounts of ethanol from xylose. Review describes how the mentioned drawbacks could be overcome.

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Ethanol production from selected lignocellulosic hydrolysates by genome shuffled strains of Scheffersomyces stipitis

Cited by 29 publications

References 18 publications

Adaptation of Scheffersomyces stipitis to hardwood spent sulfite liquor by evolutionary engineering

Adaptation of Scheffersomyces stipitis to hardwood spent sulfite liquor by evolutionary engineering

The isolation of pentose-assimilating yeasts and their xylose fermentation potential

Construction of advanced producers of first- and second-generation ethanol in Saccharomyces cerevisiae and selected species of non-conventional yeasts (Scheffersomyces stipitis, Ogataea polymorpha)

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