Ethanol production from sweet sorghum by Saccharomyces cerevisiae DBKKUY-53 immobilized on alginate-loofah matrices

Abstract

Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed. As found in this study, an ALM with dimensions of 20×20×5mm is effective for cell immobilization due to its compact structure and long-term stability. The ALM-immobilized cell system exhibited greater ethanol production efficiency than the freely suspended cell system. By using a central composite design (CCD), the optimum conditions for ethanol production from SSJ by ALM-immobilized cells were determined. The maximum ethanol concentration and volumetric ethanol productivity obtained using ALM-immobilized cells under the optimal conditions were 97.54g/L and 1.36g/Lh, respectively. The use of the ALM-immobilized cells was successful for at least six consecutive batches (360h) without any loss of ethanol production efficiency, suggesting their potential application in industrial ethanol production.

Sustainable Ethanol Production From Sugarcane Molasses by Saccharomyces cerevisiae Immobilized on Chitosan-Coated Manganese Ferrite

“…It is likely, that in the system with the agglomerated yeast cells coated with nanoparticles, an ethanol concentration gradient is achieved, preventing the inhibition of fermentation. Nuanpeng et al (2018) studied the influence of immobilization of Saccharomyces by entrapment in 2% sodium alginate gel on ethanol production, observing that immobilization favored ethanol production. Zheng et al (2012) using S. cerevisiae immobilized in an alginate-based MCM-41 mesoporous zeolite to ferment sugar molasses, obtained 78.6 g/L as the highest ethanol concentration.…”

Characterization and biological activities of polysaccharides extracted from the filamentous fungal cell wall: an updated literature review

“…Qi et al (2018) used S. cerevisiae cocultures to improve the solvents production from cassava without adding amylolytic enzymes, increasing the fermentation processes productivity. Nuanpeng et al (2018) used a thermotolerant S. cerevisiae strain immobilized in an alginate matrix for ethanol production from sweet sorghum juice, providing with a potential industrial application. Plant-derived oleanolic acid production has been effectively boosted via engineered S. cerevisiae to optimize the fermentation, transcriptional level of key genes, oxidation-reduction system, and increase over 7.5-fold compared to the maximum production reported (Zhao et al, 2018).…”

Possibilities and Prospects Regarding Ethanol Production from Saccharin Sorghum [Sorghum bicolor (L.) Moench]

“…Thus, it is necessary to develop studies with alternative crops that can supply the industrial sector with adequate amounts of raw materials, including extending the industrial processing period, with costs and efficiencies compatible with the market [24]. Several alternative raw materials have been studied and shown to be efficient in alcohol production, such as sugar-based materials (e.g., cane syrups, beet molasses), starch-based materials (e.g., cassava, corn, potatoes), and lignocellulosic materials (e.g., rice straw; sugarcane bagasse; corn stalks; grasses; pineapple stems, leaves and husks), in addition to sugar cane, cassava, and sorghum [25]. Sorghum offers tremendous potential as a raw material for the production of fuels and chemicals from cell wall sugars and biopolymers, whereas its structural and compositional heterogeneity within the plant may allow physical fractions to adapt the properties of the raw material to a biorefining process [26].…”