Ethanol stimulates trehalose production through a SpoT-DksA-AlgU dependent pathway in<i>Pseudomonas aeruginosa</i>

Harty, Colleen E.; Martins, Dorival; Doing, Georgia; Mould, D.L.; Clay, Michelle E.; Nguyen, Dao M.; Hogan, Deborah A.

doi:10.1101/523126

Cited by 4 publications

(2 citation statements)

References 125 publications

(160 reference statements)

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“…The latter result seems consistent with up-regulation of (p)ppGpp synthetase I (SpoT/RelA, Clocel_2071) in butanol-supplemented C. cellulovorans cultures, which suggests an increase of cellular levels of (p)ppGpp that mediates the so called stringent response (Schäfer et al, 2020). (p)ppGpp is involved in bacterial response to multiple stresses, including nutrient limitation, heat shock and ethanol (Harty et al, 2019;Schäfer et al, 2020). Increased levels of (p)ppGpp have been reported to modulate many aspects of bacterial physiology and metabolism, including inhibition of transcription, translation, GTP biosynthesis, DNA replication and microbial growth (Potrykus and Cashel, 2008;Schäfer et al, 2020).…”

Section: Proteins Involved In Translation and Ribosome Structuresupporting

confidence: 70%

Clostridium cellulovorans Proteomic Responses to Butanol Stress

Costa

Usai

et al. 2021

Front. Microbiol.

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Combination of butanol-hyperproducing and hypertolerant phenotypes is essential for developing microbial strains suitable for industrial production of bio-butanol, one of the most promising liquid biofuels. Clostridium cellulovorans is among the microbial strains with the highest potential for direct production of n-butanol from lignocellulosic wastes, a process that would significantly reduce the cost of bio-butanol. However, butanol exhibits higher toxicity compared to ethanol and C. cellulovorans tolerance to this solvent is low. In the present investigation, comparative gel-free proteomics was used to study the response of C. cellulovorans to butanol challenge and understand the tolerance mechanisms activated in this condition. Sequential Window Acquisition of all Theoretical fragment ion spectra Mass Spectrometry (SWATH-MS) analysis allowed identification and quantification of differentially expressed soluble proteins. The study data are available via ProteomeXchange with the identifier PXD024183. The most important response concerned modulation of protein biosynthesis, folding and degradation. Coherent with previous studies on other bacteria, several heat shock proteins (HSPs), involved in protein quality control, were up-regulated such as the chaperones GroES (Cpn10), Hsp90, and DnaJ. Globally, our data indicate that protein biosynthesis is reduced, likely not to overload HSPs. Several additional metabolic adaptations were triggered by butanol exposure such as the up-regulation of V- and F-type ATPases (involved in ATP synthesis/generation of proton motive force), enzymes involved in amino acid (e.g., arginine, lysine, methionine, and branched chain amino acids) biosynthesis and proteins involved in cell envelope re-arrangement (e.g., the products of Clocel_4136, Clocel_4137, Clocel_4144, Clocel_4162 and Clocel_4352, involved in the biosynthesis of saturated fatty acids) and a redistribution of carbon flux through fermentative pathways (acetate and formate yields were increased and decreased, respectively). Based on these experimental findings, several potential gene targets for metabolic engineering strategies aimed at improving butanol tolerance in C. cellulovorans are suggested. This includes overexpression of HSPs (e.g., GroES, Hsp90, DnaJ, ClpC), RNA chaperone Hfq, V- and F-type ATPases and a number of genes whose function in C. cellulovorans is currently unknown.

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Section: Proteins Involved In Translation and Ribosome Structuresupporting

confidence: 70%

Clostridium cellulovorans Proteomic Responses to Butanol Stress

Costa

Usai

et al. 2021

Front. Microbiol.

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“…Cold-shock can also induce trehalose synthesis by the otsBA system [12]. Exposure to ethanol or NaCl stress can induce trehalose biosynthesis in P. aeruginosa via the TreYZ pathway [13].…”

Section: Trehalose Biosynthesis Pathwaysmentioning

confidence: 99%

Trehalose and bacterial virulence

Vanaporn

Titball

2020

Virulence

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Trehalose is a disaccharide of two D-glucose molecules linked by a glycosidic linkage, which plays both structural and functional roles in bacteria. Trehalose can be synthesized and degraded by several pathways, and induction of trehalose biosynthesis is typically associated with exposure to abiotic stress. The ability of trehalose to protect against abiotic stress has been exploited to stabilize a range of bacterial vaccines. More recently, there has been interest in the role of this molecule in microbial virulence. There is now evidence that trehalose or trehalose derivatives play important roles in virulence of a diverse range of Gram-positive and Gram-negative pathogens of animals or plants. Trehalose and/or trehalose derivatives can play important roles in host colonization and growth in the host, and can modulate the interactions with host defense mechanisms. However, the roles are typically pathogen-specific. These findings suggest that trehalose metabolism may be a target for novel pathogen-specific rather than broad spectrum interventions.

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Life on the leaf: Seasonal activities of the phyllosphere microbiome of perennial crops

Howe

Stopnišek

et al. 2021

Preprint

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Plants and microbes form beneficial associations. It is expected that understanding these interactions will allow for microbiome management to enhance crop productivity and resilience to stress. Here, we apply a genome-centric approach to identify key leaf microbiome members and quantify their activities on field-grown switchgrass and miscanthus. We integrate metagenome and metatranscriptome sequencing from 237 leaf samples collected over key time points in crop phenology. We curate metagenome-assembled-genomes (MAGs), and conservatively focus analysis on the highest quality MAGs that were <15.5% contaminated, >70% complete, and detected in a minimum of 10% of samples for each crop. Populations represented by these MAGs were actively transcribing genes, and exhibited seasonal dynamics in key functions, including pyruvate metabolism, threonine, homoserine and serine biosynthesis, and stress response. Notably, we detected enrichment in transcripts annotated to nonmavalonate isoprene biosynthesis in the late season, prior to and during host senescence, concurrent with when plants are expected to decrease their isoprene biosynthesis. We also detected groups of MAGs that had coherent transcript dynamics in thioredoxin reductase, which suggests a response to reactive oxygen species, potentially released by plant hosts experiencing abiotic stress. Overall, this study overcame laboratory and bioinformatic challenges associated with field-based leaf metatranscriptomes analysis to direct to some of the key activities of phyllosphere bacteria. These activities collectively support that leaf-associated bacterial populations can be seasonally dynamic, responsive to host cues, and, likely, interactively engage in feedbacks with the plant.

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Ethanol stimulates trehalose production through a SpoT-DksA-AlgU dependent pathway inPseudomonas aeruginosa

Cited by 4 publications

References 125 publications

Clostridium cellulovorans Proteomic Responses to Butanol Stress

Clostridium cellulovorans Proteomic Responses to Butanol Stress

Trehalose and bacterial virulence

Life on the leaf: Seasonal activities of the phyllosphere microbiome of perennial crops

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