Ethanolic extract Ocimum sanctum. Enhances cognitive ability from young adulthood to middle aged mediated by increasing choline acetyl transferase activity in rat model

Kusindarta, Dwi Liliek; Wihadmadyatami, Hevi; Jadi, Arvendi Rahma; Karnati, Srikanth; Lochnit, Guenter; Hening, Puspa; Haryanto, Aris; Auriva, Made B.; Purwaningrum, Medania

doi:10.1016/j.rvsc.2018.04.005

Cited by 14 publications

(8 citation statements)

References 37 publications

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“…In Indonesia, Ocimum sanctum is very easy to find and use commonly as vegetable food. It is well-known that Ocimum sanctum acts as a chemopreventive, anti-carcinogenic, free radical scavenger and also used as a medication for neurodegenerative diseases [15, 16, 17, 18].…”

Section: Introductionmentioning

confidence: 99%

Ethanolic extract Ocimum sanctum Linn. induces an apoptosis in human lung adenocarcinoma (A549) cells

Wihadmadyatami

Karnati

Hening

et al. 2019

Heliyon

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Ocimum sanctum (OS) is tropical herbal plant which is easy to find and widely used as a vegetable food in Indonesia. In last decade, lung adenocarcinoma was in top position as male cancer disease in Indonesia. Recently, emerging data showing the extracts of different species of Ocimum exhibiting the anti-tumor properties. Further studies on lung lewis carcinoma demonstrated pro-apoptosis effects after the treatment with Ocimum extracts. However, the effect of OS of Indonesian origin in human alveolar pulmonary adenocarcinoma A549 cells remain unclear. Therefore, we aimed to investigate effects of ethanolic extract OS (EEOS) in A549 cell culture systems. Cell adhesion and viability assays revealed that EEOS significantly decreased the attachment into extracellular matrix of A549 cells. Morphological examination AO/EB and DAPI staining indicated that EEOS induced the cells shrinkage, DNA fragmentation and condensation of A549 cells. Further, EEOS treatment induced the apoptosis rate followed by up-regulation of reactive oxygen species (ROS), caspase-3 expression and decreased anti-apoptotic protein Bcl-2. This condition also suppressed the expression of SOD2 as well as the GPx. In conclusion, our findings indicate that EEOS suppressed the viability of A549 cells, which may result from the activation of ROS promoting the apoptosis signaling via mitochondrial intrinsic pathway. Taken together, EEOS might be a good therapeutic potential to further understand its properties in the treatment of lung carcinoma.

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Section: Introductionmentioning

confidence: 99%

Ethanolic extract Ocimum sanctum Linn. induces an apoptosis in human lung adenocarcinoma (A549) cells

Wihadmadyatami

Karnati

Hening

et al. 2019

Heliyon

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“…[ 27 ] reported that increased anticancer activity was correlated with the increase in polyphenol compounds. Many studies have investigated OS ethanolic extract and demonstrated that it comprises a broad range of polyphenols and flavonoids [ 17 , 28 , 29 ]. Based on this data, we postulate that these polyphenols and flavonoids may have roles in disrupting the interaction between MMP-2, MMP-9, and αvβ3 integrin, or alternatively, may directly decrease the expression of MMP-2, MMP-9, and αvβ3 integrin.…”

Section: Discussionmentioning

confidence: 99%

“…The specimen vouchers were saved in the Center for Agro Technology Innovation, Universitas Gadjah Mada (ID number: UGM-00430). Crude extracts and ethanolic extracts of OS were prepared as previously described [ 17 ]. The ethanolic extracts were then diluted with phosphate buffer saline (PBS) pH 7.4 to prepare the required concentrations (50 μg/mL, 70 μg/mL, 100 μg/mL, and 200 μg/mL) (Gibco, California, USA).…”

Section: Methodsmentioning

confidence: 99%

Ocimum sanctum Linn. ethanolic extract inhibits angiogenesis in human lung adenocarcinoma (a549) cells

et al. 2020

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Background and Aim: Ocimum sanctum (OS) is a herbal plant, which is easy to find and is widely used as an alternative medication. The previous studies have shown that several species of OS extract have therapeutic properties, and in some cases, antitumor properties. Furthermore, several data have shown the antiproliferative effects of OS extract in cases of breast cancer, human fibrosarcoma, and oral cancer. Lung adenocarcinoma is a major cause of male cancer worldwide; however, the effect of OS (of Indonesian origin) on the metastasis of human alveolar pulmonary adenocarcinoma A549 cells remains unclear. This study aimed to analyze the antiangiogenic effects of OS ethanolic extract in A549 lung adenocarcinoma cells. Materials and Methods: An angiogenesis assay was performed by seeding A549 cells on extracellular matrix solution and observing tube formation using an inverted microscope. Enzyme-linked immunosorbent assay for αvβ3, matrix metalloproteinase (MMP)-2, and MMP-9 was performed by analyzing the cell lysate after a given treatment. Results: OS ethanolic extract significantly inhibited tube formation of A549 cells and suppressed the expression of integrin αvβ3, MMP-2, and MMP-9. Conclusion: Our findings indicate that OS ethanolic extract disrupts angiogenesis of A549 cells, which may result from the disruption of cell migration and proliferation as a consequence of downregulation of αvβ3, MMP-2, and MMP-9. Taken together, OS ethanolic extract may represent a good therapeutic candidate for the treatment of metastasis in lung adenocarcinoma. Further studies are warranted to further establish the efficacy of OS in the treatment of lung adenocarcinoma.

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“…Increasing of ACh stimulated the increasing of pyramidal cells density in CA1 and CA3 of the hippocampus in young- and middle-aged rats that also proved ACh as a regulator of neuronal cells neurogenesis [ 20 ]. In addition, the increased expression of ChAT also helps to improve and maintain the cognitive ability of the young- and middle-aged rats [ 26 ]. The blockade of nicotinic and muscarinic ACh receptors will lead to cellular dysfunction and cell death (apoptosis) [ 6 ].…”

Section: Discussionmentioning

confidence: 99%

“…We performed MTT assay to analyze the cell viability as previously described [ 26 ]. HEK-293 cells were trypsinized, 2.5×10 4 /well counted and seeded in each well of a 96-well plate.…”

Section: Methodsmentioning

confidence: 99%

The neuroprotective effect of Ocimum sanctum Linn. ethanolic extract on human embryonic kidney-293 cells as in vitro model of neurodegenerative disease

Hening

Wijayanti

et al. 2018

Vet World

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Aim:This study aimed to analyze the neuroprotective effect of Ocimum sanctum Linn. ethanolic extract (OSE) on human embryonic kidney-293 (HEK-293) cells as the in vitro model of neurodegenerative diseases.Materials and Methods:In this research, HEK-293 cells divided into five groups consisting of normal and healthy cells (NT), cells treated with Camptothecin 500 µM as the negative control, cells treated with trimethyltin 10 µM (TMT), cells treated with OSE 75 µg/ml, and cells pre-treated with OSE 75 µg/ml then induced by TMT 10 µM (OSE+TMT). MTT assay and phase contrast microscopy were applied to observe the cell viability quantitatively and morphological after Ocimum sanctum Linn extract treatment. Finally, the reverse transcription polymerase chain reaction was employed to study the expression of choline acetyltransferase (ChAT).Results:The MTT assay and phase contrast microscopy showed that OSE pre-treatment significantly increased the viability of TMT-induced apoptotic cells and maintained cell viability of the normal HEK-293 cells. Expression of ChAT markedly reduced on TMT treatment group, but OSE administration stabilized ChAT expression in TMT-induced HEK-293 cells.Conclusion:This present study proved that OSE administration has neuroprotective effect by increased HEK-293 cells viability and maintain ChAT expression.

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Ethanolic extract Ocimum sanctum. Enhances cognitive ability from young adulthood to middle aged mediated by increasing choline acetyl transferase activity in rat model

Cited by 14 publications

References 37 publications

Ethanolic extract Ocimum sanctum Linn. induces an apoptosis in human lung adenocarcinoma (A549) cells

Ethanolic extract Ocimum sanctum Linn. induces an apoptosis in human lung adenocarcinoma (A549) cells

Ocimum sanctum Linn. ethanolic extract inhibits angiogenesis in human lung adenocarcinoma (a549) cells

The neuroprotective effect of Ocimum sanctum Linn. ethanolic extract on human embryonic kidney-293 cells as in vitro model of neurodegenerative disease

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