Ethanolic Garlic Extract (Allium sativumL) Increased Viability and Proliferation of Human Gingival Fibroblast In Vitro

Bramanti, Indra; Sudarso, Isr; Wahyuningsih, Mae Sri Hartati; Wibawa, Tri; Karina, VM; Kusumawardani, Banun

doi:10.3329/bjms.v17i4.38315

Bangladesh J Med Sci

2018

DOI: 10.3329/bjms.v17i4.38315

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Ethanolic Garlic Extract (Allium sativumL) Increased Viability and Proliferation of Human Gingival Fibroblast In Vitro

Indra Bramanti¹,

Isr Sudarso²,

Mae Sri Hartati Wahyuningsih³

et al.

Abstract: Introduction: Garlic is a natural herb which can be used to be a good alternative treatment because cheap and safe. Garlic contains allicin which may has act antibacterial and antiinflammatory effect. Moreover, garlic extract has a good biocompatibility and can stimulate cell growth. Does garlic extract biocompatible and can stimulate cell growth that is seen from the proliferation of human gingival fibroblasts and how its work will be studied.Objective: The aim of this study was to analyze the biocompatibilit… Show more

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2024

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Cited by 2 publications

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Prospective and challenges of locally applied repurposed pharmaceuticals for periodontal tissue regeneration

El-Nablaway,

Rashed,

Taher

et al. 2024

Front. Bioeng. Biotechnol.

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Periodontitis is a persistent inflammatory condition that causes periodontal ligament degradation, periodontal pocket development, and alveolar bone destruction, all of which lead to the breakdown of the teeth’s supporting system. Periodontitis is triggered by the accumulation of various microflora (especially anaerobes) in the pockets, which release toxic substances and digestive enzymes and stimulate the immune system. Periodontitis can be efficiently treated using a variety of techniques, both regional and systemic. Effective therapy is dependent on lowering microbial biofilm, minimizing or eradicating pockets. Nowadays, using local drug delivery systems (LDDSs) as an adjuvant therapy to phase I periodontal therapy is an attractive option since it controls drug release, resulting in improved efficacy and lesser adverse reactions. Choosing the right bioactive agent and mode of delivery is the foundation of an efficient periodontal disease management approach. The objective of this paper is to shed light on the issue of successful periodontal regeneration, the drawbacks of currently implemented interventions, and describe the potential of locally delivered repurposed drugs in periodontal tissue regeneration. Because of the multiple etiology of periodontitis, patients must get customized treatment with the primary goal of infection control. Yet, it is not always successful to replace the lost tissues, and it becomes more challenging as the defect gets worse. Pharmaceutical repurposing offers a viable, economical, and safe alternative for non-invasive, and predictable periodontal regeneration. This article clears the way in front of researchers, decision-makers, and pharmaceutical companies to explore the potential, effectiveness, and efficiency of the repurposed pharmaceuticals to generate more economical, effective, and safe topical pharmaceutical preparations for periodontal tissue regeneration.

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Prospective and challenges of locally applied repurposed pharmaceuticals for periodontal tissue regeneration

El-Nablaway,

Rashed,

Taher

et al. 2024

Front. Bioeng. Biotechnol.

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Evaluation of Cytotoxicity of Allium sativum (Garlic Extract) against Human Dental Pulp Fibroblasts

Reddy,

Azher,

Paul

et al. 2024

International Journal of Clinical Pediatric Dentistry

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Background Vital pulp therapy procedures in primary dentition focuses on preservation and maintenance of pulp tissue that has been compromised due to caries, trauma, etc. Several pulp dressing materials have been used in primary teeth and some natural materials from the field of traditional medicine have also been introduced as medicaments in vital pulp therapy. The understanding of biologic and cytotoxic properties of newer materials is important for safe clinical usage. The biologic compatibility of these newer materials is imperative to limit or avoid tissue irritation or degeneration. Aim To evaluate the cytotoxic effects of Allium sativum on cultured human primary dental pulp fibroblasts. Materials and methods Primary pulp fibroblasts were cultured from the pulp tissue obtained from extracted deciduous primary canines and central incisor teeth. The freshly prepared concentrations of 1000, 500, 250, 125, and 62.5 µg/mL A. sativum extract were added to the 96-well plate in triplicates to which culture medium containing fourth passage cell suspension was added previously. Cells without treatment served as control, while cells treated with 5% dimethyl sulfoxide (DMSO) served as toxic control. After the addition of experimental and control agents, cells were incubated for 24 and 48 hours at 37°C in 5% CO 2 atmosphere. After the incubation period, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to determine the number of viable cells. Absorbance was read with a microplate reader at 570 nm wavelength and the relative viability of dental pulp fibroblasts at various concentrations was expressed as color intensity of the experimental wells relative to that of control. The percentage of cell viability was also calculated accordingly. Results The MTT assay results revealed that A. sativum extract, in all the concentrations tested at both the time intervals maintained a cell viability of greater than 90%. At 24 hours, the mean absorbance value of untreated control wells was recorded as 0.84400 ± 0.00916 with 100% cell viability. Among all the concentrations of garlic extract tested, highest mean absorbance value of 0.83933 ± 0.00550 with 99.44% cell viability was recorded for 62.5 µg/mL concentration. At 48 hours, the mean absorbance value of untreated control wells was recorded as 1.22767 ± 0.01106 with 100% cell viability, and the highest mean absorbance value of 1.22567 ± 0.01006 with 99.83% cell viability was recorded for 62.5 µg/mL concentration. The cell viability did not seem to be affected by the concentration of A. sativum extract at 24 hours. However, at 48 hours, the sensitivity of the cells was observed to be dependent on the concentration of A. sativum with a decrease in the viability of ...

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Ethanolic Garlic Extract (Allium sativumL) Increased Viability and Proliferation of Human Gingival Fibroblast In Vitro

Cited by 2 publications

References 14 publications

Prospective and challenges of locally applied repurposed pharmaceuticals for periodontal tissue regeneration

Prospective and challenges of locally applied repurposed pharmaceuticals for periodontal tissue regeneration

Evaluation of Cytotoxicity of Allium sativum (Garlic Extract) against Human Dental Pulp Fibroblasts

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