Introduction: Garlic is a natural herb which can be used to be a good alternative treatment because cheap and safe. Garlic contains allicin which may has act antibacterial and antiinflammatory effect. Moreover, garlic extract has a good biocompatibility and can stimulate cell growth. Does garlic extract biocompatible and can stimulate cell growth that is seen from the proliferation of human gingival fibroblasts and how its work will be studied.Objective: The aim of this study was to analyze the biocompatibility of garlic extract by observing the viability and proliferation of human gingival fibroblasts in vitro.Methods: Biocompatibility test was conducted using serial concentration of garlic extract. Human gingival fibroblasts was seeded into 96 microwell plate with density of 2x103 cells, added with the fourteen serial concentration of garlic extract, and incubated in 37o C and 5% CO2for 24, 48 and 72 hours. MTT assay was used to analyze the viability and proliferation of human gingival fibroblasts. Data were analyzed by the Kruskal Wallis and U Mann-Whitney test.Results: The result showed that in each time of observation, there is no significant difference in viability fibroblast (p>0,05), but there are significant difference between time of observation at 24, 48, and 72 hours (p <0.05).Data showed that all concentration of garlic extract increased the viability and proliferation of human gingival fibroblasts.Conclusions: The ethanolic garlic extract has a good biocompatibility to human gingival fibroblasts culture cell and can stimulate the proliferation of human gingival fibroblast.Bangladesh Journal of Medical Science Vol.17(4) 2018 p.556-561

This study aimed to compare the antibacterial effectivity of calcium hydroxide and garlic extract by assessed the inhibitory effect on clinical isolate bacterial of necrotic primary teeth.Materials and methods: Clinical isolate bacterial of primary teeth were taken from the children who visited the Clinic of Pediatric Dentistry, Dental Hospital of UGM Prof Soedomo, Yogyakarta, Indonesia with age 4-7 years old. Aerobic and anaerobic bacteria were taken directly from 26 children who will undergo endodontic treatment with a diagnosis of pulp necrosis. The identification and isolation technique was done based on CLSI Standardized Method 2012 and Biochemical identification by Thermo Scientific RapID Systems 2010. There are 3 clinical isolates bacterial of necrotic primary teeth as reference for in vitro antibacterial activity testing and 2 bacteria as a comparison. Antibacterial effectivity of calcium hydroxide was examined by diffusion method by comparing calcium hydroxide, 20%, 40%, 60%, 80% concentration of garlic extract, and garlic filtrate. The sensitivity test result was taken after 24-48 hours of incubation by assessing the inhibitory zones around the well.Results: The measurement result of inhibitory diameter zone of Calcium hydroxide against Prevotella bivia was 30 mm, against Clostridium innocum was 30 mm, against Serratia marcescens was 23 mm. The inhibitory diameter zone of Calcium hydroxide against Clostridium perfringens as a comparative bacteria was 34 mm and 30 mm against Streptococcus mutans.Conclusion: All groups in this study have inhibitory effect against clinical isolate bacterial of necrotic primary teeth. The inhibitory effect of calcium hydroxide is greater than the inhibitory effect of garlic extract but lower when compared with garlic filtrate.International Journal of Human and Health Sciences Vol. 04 No. 04 October’20 Page : 282-286

Background : This study aims to compare the ability of two medicaments between cresophene and garlic on antibacterial activity of isolate deciduous necrotic teeth.Methods: In vitro test to see the antibacterial activity was carried out on the aerobic and anaerobic bacteria taken straight from the necrotic tooth of pediatric patients (aged 4-7 years old) that treated for endodontics in Pediatric Dental Clinic of RSGMUGM Prof Soedomo. Bacterial identification using the CLSI Standardized Method 2012 and Biochemical identification by Thermo Scientific Rapid System 2010 guideline. In this identification, was founded three species: Prevotella bivia, Serratia marcescens and Clostridium innocum. This test was carried out quantitative measurements to see the inhibition zone of bacterial growth. Whether cresophene has antibacterial potential test results from using an analytical descriptive test that was tabulated.Study group treated with cresophene in a well of 0.7 cm at a dose of 75 μL, garlic extract at a dose of 75 μL divided into five groups with concentrations of 20%, 40%, 60%, 80%, and garlic filtrate. Comparison of the cresophene and garlic effect conducted in vitro by looking at the inhibition zone of the bacterial growth.Results: In all groups, there are inhibitory zones. Cresophene against bacteria Prevotella bivia (54mm), Clostridium innocum (30 mm), Clostridium perfringens (26 mm), Serratia macescens (26 mm), S.mutans (54 mm), while garlic extract against bacteria Prevotella bivia, Clostridium innocum, Clostridium perfringens, S.mutansdonot show againt inhibitory, Serratia macescens (10mm, in concentration of 80 %) and garlic filtrate against bacteria Prevotella bivia (51 mm), Clostridium innocum (28 mm), Clostridium perfringens (46 mm), Serratia marcescens (31 mm), S.mutans (49 mm).Conclusion: Cresophene has a greater inhibition than garlic extract, but it is lower than garlic filtrate.International Journal of Human and Health Sciences Vol. 04 No. 04 October’20 Page : 287-290