Ethanolic Neem (<i>Azadirachta indica</i>) Leaf Extract Prevents Growth of MCF-7 and HeLa Cells and Potentiates the Therapeutic Index of Cisplatin

Sharma, Chhavi; Vas, Andrea J.; Goala, Payal; Gheewala, Taher; Rizvi, Tahir A.; Hussain, Arif

doi:10.1155/2014/321754

Cited by 50 publications

(41 citation statements)

References 46 publications

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“…The combination of neem-derived gedunin and cisplatin further decreases the proliferation of treated ovarian cancer cells by almost 50% compared to the cells treated with only cisplatin [28]. The combination of sub-lethal dose of ethanolic NLE and cisplatin also provides synergistic effects in decreasing the viability of breast and cervical cancer cells compared to individual compound alone [100]. …”

Section: Combination Of Neem Components With Other Therapiesmentioning

confidence: 99%

“…The susceptibility of tumor cells to MEX may be associated with the modification of the plasma membrane lipids during transformation of normal cells to malignant cells. The ethanolic neem leaf extract also specifically targets the breast as well as cervical cancer cells, without showing significant effects on the viability of primary lymphocytes [100]. …”

Section: Therapeutic Safety and Tumor Selective Properties Of Neementioning

confidence: 99%

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Neem components as potential agents for cancer prevention and treatment

Fang

Kumar

Yadav

et al. 2014

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer

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Azadirachta indica, also known as neem, is commonly found in many semi-tropical and tropical countries including India, Pakistan, and Bangladesh. The components extracted from neem plant have been used in traditional medicine for the cure of multiple diseases including cancer for centuries. The extracts of seeds, leaves, flowers, and fruits of neem have consistently shown chemopreventive and antitumor effects in different types of cancer. Azadirachtin and nimbolide are among the few bioactive components in neem that have been studied extensively, but research on a great number of additional bioactive components is warranted. The key anticancer effects of neem components on malignant cells include inhibition of cell proliferation, induction of cell death, suppression of cancer angiogenesis, restoration of cellular reduction/oxidation (redox) balance, and enhancement of the host immune responses against tumor cells. While the underlying mechanisms of these effects are mostly unclear, the suppression of NF-κB signaling pathway is, at least partially, involved in the anticancer functions of neem components. Importantly, the anti-proliferative and apoptosis-inducing effects of neem components are tumor selective as the effects on normal cells are significantly weaker. In addition, neem extracts sensitize cancer cells to immunotherapy and radiotherapy, and enhance the efficacy of certain cancer chemotherapeutic agents. This review summarizes the current updates on the anticancer effects of neem components and their possible impact on managing cancer incidence and treatment.

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Section: Combination Of Neem Components With Other Therapiesmentioning

confidence: 99%

Section: Therapeutic Safety and Tumor Selective Properties Of Neementioning

confidence: 99%

Neem components as potential agents for cancer prevention and treatment

Fang

Kumar

Yadav

et al. 2014

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer

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“…Manasathien et al (2012) assayed the cytotoxic activity of fruit peel extract of P. granatum on Brine shrimp and determined CC-50 of 1205.98 and 1294.88 lg/ml with ethanolic and water extracts respectively. Sharma et al (2014) reported EC-50 of ethanolic neem leaf extract for MCF-7 cells at 350 lg/ml after 72 h treatment whereas in HeLa cells it was found to be175 lg/ ml in 48 h.…”

Section: Discussionmentioning

confidence: 99%

Anthelmintic potential of Calotropis procera, Azadirachta indica and Punica granatum against Gastrothylax indicus

et al. 2015

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Anthelmintic activity of both ethanolic and aqueous extracts of Calotropis procera flowers, Azadirachta indica leaves and Punica granatum fruit peel in comparison with albendazole was evaluated through in vitro studies by the worm motility inhibition assay. Significant anthelmintic effects (p \ 0.0005) were observed on live Gastrothylax indicus worm as evident from their mortality at 4 h post exposure to both ethanolic and aqueous extracts. Phytochemical analysis of extracts revealed the presence of phenols, alkaloids, saponins, tannins, flavonoids, steroids and triterpenoids. LC-50 values were determined to be 12.05 mg/ml ± 3.24 and 23.52 mg/ ml ± 6.4 for C. procera, 24.37 mg/ml ± 4.11 and 21.02 mg/ml ± 4.6 for A. indica, 18.92 mg/ml ± 4.54 and 24.43 mg/ml ± 6.96 for P. granatum ethanolic and aqueous extracts respectively, whereas it was 29.23 lg/ ml ± 4.51 for albendazole. The mean mortality index (MI) was 1.0 and 0.90 for C. procera, 0.90 for A. indica and 0.73 and 0.80 for P. granatum ethanolic and aqueous extracts respectively whereas for albendazole it was 1.0. Percent mean worm motility inhibition (%WMI) was observed to be between 70 and 100 % for different extracts.Various concentrations (5-5000 lg/ml) of all the plant extracts and albendazole were used to detect their cytotoxic effects against HeLa cell line to determine CC-50 by MTT assay. CC-50 values, of all the plant extracts were determined to be [1000 lg/ml and for albendazole it was found to be [10 lM. All the three plants can be potential sources for novel anthelmintics.

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“…Studies have shown that A. indica has got a chemo preventive capability by regression of the DEN/AAF (diethyl nitrosamine/2-acetylaminofluorene) induced carcinogenesis in liver [14,15]. These results indicate that dietary use of extracts from various parts of A. indica may play a promising role in future drug discovery and development programs as far as chemoprevention of cancer is concerned.…”

Section: Azadirachta Indica [5]mentioning

confidence: 99%

Ayurveda as an Adjuvant Medication for Combating Cancer: A Review

Goel¹,

Ojha²,

Tiwari³

2015

J Homeop Ayurv Med

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Cancer is one of the most dreaded diseases of the 20 th century and spreading further with continuance and increasing incidence in 21 st century. At present Chemoprevention and Radiotherapy is main stay of management. But these can produce toxic side effects, which have limited their extensive use. Ayurveda drugs available in different part of the world have been extensively studied for their anti-cancer activity. Ayurveda also have provided with many such drugs which can be proved to be a good anti-cancer substitute to conventional treatment or also provide benefit as an adjuvant therapy. Researches on many such Ayurvedic plants available in India like Azadirachta indica, Carica papaya, Plumbago zeylanica, Ocimum sanctum, Tinospora cordifolia, Catharanthus roseus are reviewed, and found to be effective as anti-cancer in many types of cancers and adjuvant therapy along with chemotherapy and radiotherapy. There is a broad scope to derive the potent anticancer agents from medicinal plants, which need thorough research.

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Ethanolic Neem (Azadirachta indica) Leaf Extract Prevents Growth of MCF-7 and HeLa Cells and Potentiates the Therapeutic Index of Cisplatin

Cited by 50 publications

References 46 publications

Neem components as potential agents for cancer prevention and treatment

Neem components as potential agents for cancer prevention and treatment

Anthelmintic potential of Calotropis procera, Azadirachta indica and Punica granatum against Gastrothylax indicus

Ayurveda as an Adjuvant Medication for Combating Cancer: A Review

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