This article presents the results of empirical research on the relationship of motivation for ethno-cultural continuity (MEC) and strategies of acculturation among two generations of the Russian minority in Latvia. We sampled 107 Russian families (mothers: N = 107, age = 35-59, M = 42 years; late adolescents and youth: N = 107, age = 16-24, M = 17 years). The questionnaire included measures of motivation for ethno-cultural continuity, acculturation strategies, sociocultural adaptation, and self-esteem. A path model showed that motivation for ethno-cultural continuity, preference for assimilation, self-esteem, and sociocultural adaptation of mothers significantly related to those of their children. A motivation for ethno-cultural continuity of mothers predicted their preference for integration and self-esteem, while a motivation for ethno-cultural continuity of adolescents predicted their preference for separation. Preference for integration promoted better sociocultural adaptation and self-esteem in both generations. The results allowed consideration of the process of acculturation on the three interrelated levels: individual, family, and ethnic group, with the central role of the family, teaching younger generations to maintain heritage culture and successfully integrate in the larger society.