Ethnobotany provides key insights from ancient traditions, particularly focusing on spice seeds. Despite their growing commercial value in food and pharmaceuticals, there is still a need for thorough research into their traditional uses and scientifically validated properties. The objective of this chapter is to compile and integrate current ethnobotanical knowledge with recent scientific findings and highlight the inherent medicinal and pharmacological properties of these plants. A comprehensive examination was conducted on a range of spice seeds. The traditional applications, methods of preparation, and cultural significance of these plants have been described, and the latest scientific findings have confirmed their therapeutic potential. In summary, numerous spice seeds are traditionally considered healing agents, and modern research has confirmed their benefits. The convergence of ethnobotanical knowledge and current scientific advancements has established the foundation for comprehensive integration into modern medicinal practices.