Ethyl Acetate Fraction from Hedyotis diffusa plus Scutellaria barbata Suppresses Migration of Bone‐Metastatic Breast Cancer Cells via OPN‐FAK/ERK/NF‐κB Axis

Fang, Ting; Yan, Yingxuan; Yang, Yue; Lv, Ya-Xin; Chang, Qing-Qi; Zhang, Dandan

doi:10.1155/2020/3573240

Cited by 7 publications

(3 citation statements)

References 31 publications

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“…HDE may share a similar anticancer mechanism with BIIB021, but further investigation is required. In addition, HDE is extracted from the natural herb Hedyotis diffusa, which has been used for cancer treatment in China for centuries (46). Previous studies have demonstrated that TCHD could induce apoptosis in leukemia cell lines Kasumi-1, KG-1, THP-1, U937 and K562 (47,48).…”

Section: Discussionmentioning

confidence: 99%

Combination of HDE and BIIB021 efficiently inhibits cell proliferation and induces apoptosis via downregulating hTERT in myelodysplastic syndromes

et al. 2021

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Treatment for higher-risk patients with myelodysplastic syndrome (MDS) should aim to modify the disease course by avoiding progression to acute myeloid leukemia and improving survival. When a patient is not eligible for intensive chemotherapy and lacks a donor hematopoietic cell source, or for a patient in a poor economic situation, consideration can be given to the use of Chinese herbal medicine. Numerous plant extracts, such as camptothecin, vinblastine and paclitaxel, have been reported to display antitumor effects, serving as potential therapeutic strategies for cancer. In the present study, the ultra-performance liquid chromatography-tandem mass spectrometry system (Waters Corporation) was used to detect the main chemical components of HDE, CCK-8 assay to detect the effects of HDE and BIIB021 on the proliferation of SKM-1 cells; and designed hTERT-small interfering (si) RNAs to detect the effects of HDE and BIIB021 on SKM-1 cell apoptosis after HTERT gene knockdown. The present study investigated a newly extracted coumarin HDE, the active component in Oldenlandia diffusa Willd, which efficiently inhibited SKM-1 (MDS cell line) proliferation and induced apoptosis, as determined by performing Cell Counting Kit-8 and flow cytometry assays, respectively. The effect of HDE was associated with decreased telomerase activity. Moreover, heat shock protein 90 inhibitor BIIB021 significantly enhanced the antitumor effects of HDE on SKM-1 cells. In addition, SKM-1 cell apoptosis was increased in human telomerase reverse transcriptase (hTERT)-knockdown cells compared with the negative control group. Cell apoptosis in hTERT-knockdown SKM-1 cells was further enhanced following HDE, BIIB021 or combination treatment, as evidenced by increased levels of cleaved caspase 3, cleaved caspase 8 and cleaved poly ADP ribose polymerase. Collectively, the results indicated synergistic antitumor effects of HDE and BIIB021, providing a novel therapeutic combination for higher-risk MDS.

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Section: Discussionmentioning

confidence: 99%

Combination of HDE and BIIB021 efficiently inhibits cell proliferation and induces apoptosis via downregulating hTERT in myelodysplastic syndromes

et al. 2021

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“…It is reported that the combined use of the two herbs is not simply superimposed, and their combined application is more effective than a single drug [16] . Modern pharmacological studies found that SB plus HD herb pair (SBHD) could induce cancer apoptosis, inhibit cancer cell proliferation, and suppresses cancer cells migration [17][18][19][20] . In recent years, the two herbs and their extracts have been widely used in the treatment of esophageal cancer and have shown remarkable effects [21][22][23] .…”

Section: Introductionmentioning

confidence: 99%

Integrated bioinformatics and network pharmacology to identify the therapeutic target and molecular mechanisms of Scutellaria barbata plus Hedyotis diffusa herb pair on esophageal squamous cell carcinoma

Xing

Zhang

et al. 2023

Preprint

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Objective: This study aims to systematically investigate the therapeutic targets and molecular mechanisms of Scutellaria barbata plus Hedyotis diffusa herb pair (SBHD) on esophageal squamous cell carcinoma (ESCC)based on GEO gene microarray combined with network pharmacology and molecular docking technology. Methods: The active components and effective targets of SBHD were retrieved and downloaded from the TCMSP database, and the differentially expressed genes (DEGs) of ESCC were retrieved and downloaded from the GEO database. The intersection targets between medicine target genes and disease target genes were screened by drawing Venn diagram. Bioinformatics tools such as R language, Cytoscape software, STRING platform, and DAVID platform, were applied to perform active components-targets regulatory network analysis, PPI network analysis, and GO and KEGG pathway enrichment analysis. Molecular docking was performed to validate the interaction between the core active components and the key target genes by AutoDock Vina tools. Results: A total of 33 main active componentswere predicted from herb pair, and 28 intersection targets were screened from 105 medicine target genes and 4064 disease target genes. A topological analysis of the active components-targets regulatory network and PPI network revealed 5 core ingredients and 6 key targets for SBHD treating ESCC, respectively. KEGG enrichment analysis found that SBHD could affect cellular senescence, hepatitis B, MAPK signaling pathway, proteoglycans in cancer and apoptosis in ESCC. Molecular docking found that the 5 core active compounds had good binding properties with the 6 key therapeutic targets. Conclusion: The therapeutic effects of SBHD on ESCC might be related to the active components including quercetin, baicalein, luteolin, stigmasterol and wogonin, which intervened with the key targets including IL6, CASP3, MYC, AR, CAV1 and RUNX2, and the signaling pathway including cellular senescence, hepatitis B, MAPK signaling pathway, proteoglycans in cancer and apoptosis.

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“…It is widely used as a detoxifying herb in TCM due to its functions of clearing heat, removing toxicity, activating blood and reducing swelling 13 , 14 . Accumulating evidence has proved that HD has anticancer activity in the treatment of both breast cancer and cervical cancer 15 , 16 . HD has been reported to be able to inhibit colorectal cancer growth in vivo and in vitro 17 .…”

Section: Introductionmentioning

confidence: 99%

Hedyotis diffusa–Sculellaria barbata (HD–SB) suppresses the progression of colorectal cancer cells via the hsa_circ_0039933/hsa-miR-204-5p/wnt11 axis

Zhu¹,

Yuan

Chen

2023

Sci Rep

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Our previous study confirmed that the combination of Hedyotis diffusa (HD) and Scutellaria barbata (SB) significantly inhibited colorectal cancer cell proliferation and the WNT signaling pathway. However, the exact molecular modulation remains unclear. In this study, colorectal cancer cells (SW620) were treated with 1 mg/mL HD–SB for 24 h, and high-throughput sequencing of circRNAs was performed. The level of hsa_circ_0039933 in three colorectal cancer cell lines (HT-29, SW620, and HCT116) was verified by qPCR. After transfection of hsa_circ_0039933 overexpression plasmids or small interfering RNAs, CCK8, apoptosis, cell migration, and cell invasion were utilized to evaluate the function of hsa_circ_0039933 in the progression of colorectal cancer cells. We identified hsa_circ_0039933, which was downregulated in HD–SB-induced colorectal cancer cells and positively related to colorectal cancer progression. In SW620 cells with relatively high expression of hsa_circ_0039933, interfering with the expression of hsa_circ_0039933 inhibited the proliferation, invasion, and migration of SW620 cells. In HCT116 cells with relatively low expression of hsa_circ_0039933, overexpression of hsa_circ_0039933 promoted the proliferation and invasion and migration ability of HCT116. Mechanistically, hsa_circ_0039933 targeted hsa-miR-204-5p to increase the expression of wnt11, leading to the activation of the Wnt pathway, thereby promoting the proliferation of colorectal cancer cells. This work revealed the potential molecular mechanism of HD–SB for the treatment of colorectal cancer, which was to inhibit the Wnt signaling pathway through the hsa_circ_0039933/hsa-miR-204-5p/wnt11 axis, then suppressing proliferation, migration, and invasion in the colorectal cancer cell.

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Ethyl Acetate Fraction from Hedyotis diffusa plus Scutellaria barbata Suppresses Migration of Bone‐Metastatic Breast Cancer Cells via OPN‐FAK/ERK/NF‐κB Axis

Cited by 7 publications

References 31 publications

Combination of HDE and BIIB021 efficiently inhibits cell proliferation and induces apoptosis via downregulating hTERT in myelodysplastic syndromes

Combination of HDE and BIIB021 efficiently inhibits cell proliferation and induces apoptosis via downregulating hTERT in myelodysplastic syndromes

Integrated bioinformatics and network pharmacology to identify the therapeutic target and molecular mechanisms of Scutellaria barbata plus Hedyotis diffusa herb pair on esophageal squamous cell carcinoma

Hedyotis diffusa–Sculellaria barbata (HD–SB) suppresses the progression of colorectal cancer cells via the hsa_circ_0039933/hsa-miR-204-5p/wnt11 axis

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