Ethylendiamintetraessigsäure (EDTA) und ihre Alkalisalze [MAK Value Documentation in German language, 2009]

doi:10.1002/3527600418.mb6000d0046

Cited by 1 publication

(1 citation statement)

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“…The relevant fractions of the test system were the exposure medium, the cell surface, the cells themselves, and the culture flask plastic. Unlike in our previous experiments, 21 we sampled the cell surface by rinsing with 400 μL Versene solution for 30 s. Versene contains the cell dissociation agent ethylenediaminetetraacetic acid (EDTA), which chelates with divalent metal ions 30 due to its four carboxylic acid groups with strong dissociation constants (pK a 0.26 to 2.76 31 ). Thus, any test compound loosely associated with the cell surface would have been sampled with EDTA upon rinsing with Versene.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Bioconcentration Assessment of Three Cationic Surfactants in Permanent Fish Cell Lines

Balk,

Hüsser,

Hollender

et al. 2024

Environ. Sci. Technol.

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Cationic surfactants are used in many industrial processes and in consumer products with concurrent release into the aquatic environment, where they may accumulate in aquatic organisms to regulatoryly relevant thresholds. Here, we aimed to better understand the bioconcentration behavior of three selected cationic surfactants, namely N,N-dimethyldecylamine (T10), N-methyldodecylamine (S12), and N,N,N-trimethyltetradecylammonium cation (Q14), in the cells of fish liver (RTL-W1) and gill (RTgill-W1) cell lines. We conducted full mass balances for bioconcentration tests with the cell cultures, in which the medium, the cell surface, the cells themselves, and the plastic compartment were sampled and quantified for each surfactant by HPLC MS/MS. Accumulation in/to cells correlated with the surfactants' alkyl chain lengths and their membrane lipid−water partitioning coefficient, D MLW . Cell-derived bioconcentration factors (BCF) of T10 and S12 were within a factor of 3.5 to in vivo BCF obtained from the literature, while the cell-derived BCF values for Q14 were >100 times higher than the in vivo BCF. From our experiments, rainbow trout cell lines appear as a suitable conservative in vitro screening method for bioconcentration assessment of cationic surfactants and are promising for further testing.

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Section: ■ Materials and Methodsmentioning

confidence: 99%