Ethylene and Ethane from Poa pratensis Callus and from Leaf Blades of Regenerated and Seed-Derived Plants Inoculated with Bipolaris sorokiniana

Hodges, Clinton F.; Stephens, Loren C.; Campbell, Douglas A.

doi:10.1016/s0176-1617(99)80326-5

Cited by 5 publications

(3 citation statements)

References 29 publications

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“…For embryo culture, mature seeds were disinfested and plated on CCm2 medium and the embryos were dissected from the seeds after 2 days imbibition, according to previously established methods (Hodges et al, 1999). Dissected embryos were plated back ont CCm2 medium for 6-8 weeks, after which they were plated ont CCmr medium, whether or not the embryos had callused.…”

Section: Methodsmentioning

confidence: 99%

Plants regenerated from embryo cultures of an apomictic clone of Kentucky bluegrass (Poa pratensis L. ‘Baron’) are not apomictic in origin

et al. 2006

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Plants were regenerated from tissue cultures of embryos dissected from seeds that were harvested from a selfpollinated clonal selection of Kentucky bluegrass (Poa pratensis L.) 'Baron', an apomictic cultivar. Plants were regenerated from 35 embryo-derived callus cultures of the 3280 embryos that were plated. Flow-cytometric (FCM) and RAPD-marker analyses were performed to determine if regenerants were or were not apomictic in origin. Fifteen regenerants with a 3c DNA content were classified as arising from 2n + n aberrant embryos, which was a higher frequency than expected, based on a chi-square analysis. Of the remaining 20 regenerants with a 2c DNA content, a chi-square test showed that all could have arisen from n + n sexually-derived embryos, based on the observed segregation of n + n regenerants, which fit the expected 3:1 ratio of dominant:recessive RAPD-marker phenotypes. The apparent lack of regenerants of apomictic origin, and implications for genetic transformation and breeding of Kentucky bluegrass are discussed.Abbreviations: n + 0: haploid seedling, 2n + 0: apomictically-derived seedling, n + n: sexually-derived seedling, 2n + n: aberrant seedling derived from unreduced egg and reduced sperm, FCM: Flow cytometry, RAPD: Randomly amplified polymorphic DNA, 1c: haploid, 2c: diploid, 3c: triploid

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Section: Methodsmentioning

confidence: 99%

Plants regenerated from embryo cultures of an apomictic clone of Kentucky bluegrass (Poa pratensis L. ‘Baron’) are not apomictic in origin

et al. 2006

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“…Over the past 20 years many culture systems for Kentucky bluegrass were reported using various tissues as explants, i.e. mature and immature zygotic embryos (Manton et al, 1982;McDonnell and Conger, 1984;Boyd and Dale, 1986), immature inflorescences ( Van der Valk et al, 1989), shoot tips (Wu and Jampates, 1986), whole mature seeds (Krans, 1981; Van der Valk et al, 1989Van Ark et al, 1991;Griffin and Dibble, 1995;Hodges et al, 1999), and coleoptile, leaf, and stem sections of seedlings . Suspension cultures of Kentucky bluegrass were also described .…”

Section: Introductionmentioning

confidence: 99%

Stable transformation of a recalcitrant kentucky bluegrass (Poa pratensis L.) cultivar using mature seed-derived highly regenerative tissues

Lemaux

Cho

2001

In Vitro Cell.Dev.Biol.-Plant

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An efficient method to produce highly regenerative tissues from seeds of a previously recalcitrant cultivar of Kentucky bluegrass (Poa pratensis L. cv. Kenblue) was established under dim-light conditions (10±30 mE m 22 s 21 , 16-h light) using media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D; 4.5 or 9.0 mM), 6-benzylaminopurine (BA; 0.44 or 2.2 mM), and a high level of cupric sulfate (5.0 mM). The tissues were co-transformed with three plasmids containing the genes for hygromycin phosphotransferase (hpt), b-glucuronidase (uidA; gus), and a synthetic green fluorescent protein gene [sgfp(S65T)]. From 463 individual explants bombarded, 10 independent transgenic events (2.2%) were obtained after a 3±4-month selection period for hygromycin resistance using 30±100 mg l 21 hygromycin B; of the 10 independent events, seven (70%) were regenerable. Stable integration of the transgene(s) in transgenic plants was confirmed by polymerase chain reaction and DNA blot hybridization analyses. Coexpression frequency of all three genes was 20%; for two transgenes, either hpt/uidA or hpt/sgfp(S65T), coexpression frequency was 30±40%.

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“…Shoots were harvested from the M 0 plant again in mid-July 2006, when inflorescences were 1 to 5 cm long within the immature flowering shoot. Following methods of Chen et al (1979), shoot sections %9 to 11 cm long were disinfected in 75% commercial liquid bleach (0.6% v/v sodium hypochlorite), rinsed four times in sterile, deionized water, and plated as longitudinal split-stem sections on CCm semisolid medium (Hodges et al, 1999) with 5 mgÁL -1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mgÁL -1 benzyladenine (BA; hereafter CCm2 5 B 1 ). After 24-h culture, inflorescences could be separated from the surrounding leaf sheaths and replated onto fresh CCm2 5 B 1 medium.…”

Section: Methodsmentioning

confidence: 99%

Ethidium Bromide-induced Mutations from Inflorescence Cultures of Indiangrass

Stephens¹

2009

horts

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Immature inflorescences of a Sorghastrum nutans (L.) Nash selection were cultured on CCm medium with 5 mg·L−1 2,4-dichlorophenoxyacetic acid and 1 mg·L−1 N6-benzyladenine (BA) for 5 weeks. Callused inflorescence cultures were placed on CCm medium with 1 mg·L−1 BA (CCmB1) and 0 or 250 mg·L−1 ethidium bromide (EtBr) for 24 h. Cultures were transferred to CCmB1 without EtBr for shoot regeneration and then to CCm without plant growth regulators for rooting. Rooted shoots were transferred to soil under greenhouse conditions and then to the field. Fifteen putative M1 mutants with atypical phenotypes were detected among 71 EtBr-treated regenerants. Two self-incompatible putative M1 mutants were progeny-tested by using a wild-type Indiangrass seedling as the pollen parent. M1 selection ISU06-35 was a dwarf mutant whose M2 testcross progeny segregated 1:1 tall:dwarf seedlings. M1 selection ISU06-56 was a red-flowered mutant whose M2 testcross progeny segregated 1:1 green-flowered:red-flowered seedlings. These results are consistent with both M1 mutants being dominant nuclear mutations.

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Ethylene and Ethane from Poa pratensis Callus and from Leaf Blades of Regenerated and Seed-Derived Plants Inoculated with Bipolaris sorokiniana

Cited by 5 publications

References 29 publications

Plants regenerated from embryo cultures of an apomictic clone of Kentucky bluegrass (Poa pratensis L. ‘Baron’) are not apomictic in origin

Plants regenerated from embryo cultures of an apomictic clone of Kentucky bluegrass (Poa pratensis L. ‘Baron’) are not apomictic in origin

Stable transformation of a recalcitrant kentucky bluegrass (Poa pratensis L.) cultivar using mature seed-derived highly regenerative tissues

Ethidium Bromide-induced Mutations from Inflorescence Cultures of Indiangrass

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