Ethylene generation and reversal of ethylene effects during development in vitro of rapid-cycling Brassica campestris L

Lentini, Zaida; Mussell, Harry; Mutschler, Martha A.; Earle, Elizabeth D.

doi:10.1016/0168-9452(88)90057-x

Cited by 57 publications

(25 citation statements)

References 13 publications

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“…Many reports showed a depressive action of CzH4 on growth and development of plants in vitro [9,14] and on regeneration in tissue cultures [10,18,20], however, several experiments have led to the opposite conclusion [5]. The main problem for a general understanding is that C2H 4 acts inside the tissues where it is produced before being released and is efficient at very low concentrations.…”

Section: Discussionmentioning

confidence: 97%

Enhancement of production and regeneration of embryogenic type II callus in Zea mays L. by AgNO3

Vain

Yean

Flament

1989

Plant Cell Tiss Organ Cult

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In order to evaluate the impact of ethylene in maize tissue culture, silver nitrate has been used as an inhibitor of ethylene action. Type II callus initiation rate was improved when immature embryos were cultured on a modified Murashige & Skoog medium containing various concentrations of silver nitrate (5, 10, 20 mg 1-J ). Regeneration ability of calli initiated and maintained in presence of silver nitrate was enhanced. No modification of callus growth rate neither of ethylene production has been detected.

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Section: Discussionmentioning

confidence: 97%

Enhancement of production and regeneration of embryogenic type II callus in Zea mays L. by AgNO3

Vain

Yean

Flament

1989

Plant Cell Tiss Organ Cult

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“…Beakers were covered with Petri plates, sealed with filter tape (Carolina Biological) and either kept in the 25 °C culture room or placed in an incubator with a 16 h photoperiod at 20 °C and 8 h dark at 15 °C. To obtain normal leaf expansion in plants grown in vitro it was necessary to use filter tape, which prevented ethylene accumulation [11], rather than Parafilm.…”

Section: Plant Materialsmentioning

confidence: 99%

Increased totipotency of protoplasts from Brassica oleracea plants previously regenerated in tissue culture

Robertson

Earle

Mutschler

1988

Plant Cell Tiss Organ Cult

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Factors affecting the division of cells derived from leaf and cotyledon protoplasts from Brassica oleracea L. var. italica ('Green Comet' hybrid broccoli) were examined to optimize conditions for plant regeneration and to determine whether there was a genetic basis for improved regeneration from protoplasts derived from plants previously regenerated from tissue cultures [15]. When leaf protoplasts from different plants grown from hybrid seed were isolated and cultured simultaneously, division efficiencies of 1-95% were obtained. Cells from some plants showed high division efficiencies in consecutive experiments while cells from other plants had consistently low division rates. More plants from hybrid seed gave high division efficiencies when cotyledon protoplasts were used. However, cotyledon or leafprotoplasts from selfed progeny of regenerated plants produced more vigorous calli and more shoots than protoplasts from hybrid seed. These results suggest that there may be a genetic component to the increased totipotency of Brassica oleracea protoplasts.

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“…Plant cells and tissues grown in culture have been known to produce ethylene (LaRue and Gamborg 1971), the accumulation of which in sealed cultured containers is inhibitory to cell differentiation and shoot regeneration in vitro (Chi et al 1991;Pun and Chi 1993) and affects normal plant growth and development (Hussey and Stacey 1981;Lentini et al 1988). The inhibition of ethylene production or action using inhibitors, e.g.…”

Section: Introductionmentioning

confidence: 99%

Enhanced de novo shoot morphogenesis in vitro by expression of antisense 1-aminocyclopropane-1-carboxylate oxidase gene in transgenic mustard plants

Pua

Lee

1995

Planta

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The role of ethylene in shoot morphogenesis in vitro was investigated via the production of transgenic mustard (Brassica juncea (L.) Czern & Coss cv. Indian Mustard) plants with impaired ethylene biosynthesis using the antisense-RNA approach. Mustard plants transformed with antisense MEFE5 encoding 1-aminocyclopropane-l-carboxylate (ACC) oxidase, which catalyses the conversion of ACC to ethylene in mustard, were characterized with respect to enzyme activity, ethylene production and the capacity for shoot morphogenesis in vitro. Most transgenic plants exhibited a decrease in the amount of endogenous 1.4-kb transcript and ethylene production, which was concomitant with a marked increase of shoot regenerability of cultured tissues. Tissues of four individual highly regenerative transgenic plants (TO, T2, T4 and T8) consistently showed lower relative activity of endogenous ACC oxidase and ethylene production than control tissues during a three-week culture period. The highly regenerative phenotype of transgenic tissues reverted to the poorly regenerative control phenotype in the presence of 50 ~tM 2-chloroethylphosphonic acid. The inverse relationship between shoot regenerability and ethylene production possessed by individual transgenic plants was also manifest in R1 progeny. This study presents strong evidence that ethylene plays a key regulatory role in the de novo shoot formation of mustard in culture, and also indicates a possible general role of ethylene in plant morphogenesis in vitro. Abbreviations: ACC= 1-aminocyclopropane-l-carboxylate; AVG = aminoethoxyvinylglycine; CEPA = 2-chloroethylphosphonic acid; GUS=[3-glucuronidase; MS=Murashige and Skoog; NC=negative control (non-transformed mustard plants); NP-TII =neomycin phosphotransferase type II; PC = positive control (transformed mustard plants carrying pROA93) Correspondence to: E.-C. Pua; Fax: 65 (779) 5671

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Ethylene generation and reversal of ethylene effects during development in vitro of rapid-cycling Brassica campestris L

Cited by 57 publications

References 13 publications

Enhancement of production and regeneration of embryogenic type II callus in Zea mays L. by AgNO3

Enhancement of production and regeneration of embryogenic type II callus in Zea mays L. by AgNO3

Increased totipotency of protoplasts from Brassica oleracea plants previously regenerated in tissue culture

Enhanced de novo shoot morphogenesis in vitro by expression of antisense 1-aminocyclopropane-1-carboxylate oxidase gene in transgenic mustard plants

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