Ethylene production and signaling in tomato (Solanum lycopersicum) pollen grains is responsive to heat stress conditions

Jegadeesan, Sridharan; Beery, Avital; Altahan, Leviah; Meir, Shimon; Pressman, Etan; Firon, Nurit

doi:10.1007/s00497-018-0339-0

Cited by 31 publications

(23 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is noteworthy that proteins involved in the regulation of protein synthesis, in oxidative stress or in detoxification displayed a profile differing from that of the corresponding transcript ( Gallardo et al, 2007 ). In addition, ethylene-related proteins (ethylene-biosynthesis, -perception, or -signaling) were not detected in either of the pollen samples used in this study, while expression of the respective genes at transcript level was recently demonstrated by us in tomato pollen ( Jegadeesan et al, 2018 ). This may be due to low abundance of the proteins in pollen.…”

Section: Resultsmentioning

confidence: 53%

Proteomics of Heat-Stress and Ethylene-Mediated Thermotolerance Mechanisms in Tomato Pollen Grains

et al. 2018

Self Cite

View full text Add to dashboard Cite

Heat stress is a major cause for yield loss in many crops, including vegetable crops. Even short waves of high temperature, becoming more frequent during recent years, can be detrimental. Pollen development is most heat-sensitive, being the main cause for reduced productivity under heat-stress across a wide range of crops. The molecular mechanisms involved in pollen heat-stress response and thermotolerance are however, not fully understood. Recently, we have demonstrated that ethylene, a gaseous plant hormone, plays a role in tomato (Solanum lycopersicum) pollen thermotolerance. These results were substantiated in the current work showing that increasing ethylene levels by using an ethylene-releasing substance, ethephon, prior to heat-stress exposure, increased pollen quality. A proteomic approach was undertaken, to unravel the mechanisms underlying pollen heat-stress response and ethylene-mediated pollen thermotolerance in developing pollen grains. Proteins were extracted and analyzed by means of a gel LC-MS fractionation protocol, and a total of 1,355 proteins were identified. A dataset of 721 proteins, detected in three biological replicates of at least one of the applied treatments, was used for all analyses. Quantitative analysis was performed based on peptide count. The analysis revealed that heat-stress affected the developmental program of pollen, including protein homeostasis (components of the translational and degradation machinery), carbohydrate, and energy metabolism. Ethephon-pre-treatment shifted the heat-stressed pollen proteome closer to the proteome under non-stressful conditions, namely, by showing higher abundance of proteins involved in protein synthesis, degradation, tricarboxylic acid cycle, and RNA regulation. Furthermore, up-regulation of protective mechanisms against oxidative stress was observed following ethephon-treatment (including higher abundance of glutathione-disulfide reductase, glutaredoxin, and protein disulfide isomerase). Taken together, the findings identified systemic and fundamental components of pollen thermotolerance, and serve as a valuable quantitative protein database for further research.

show abstract

Section: Resultsmentioning

confidence: 53%

Proteomics of Heat-Stress and Ethylene-Mediated Thermotolerance Mechanisms in Tomato Pollen Grains

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…AtERS2 gene shows a similar expression pattern to the ethylene receptor gene Os-ERS1 in rice that is up-regulated during early pollen development, corresponding to meiotic and microspore stages [22]. In tomato, the ethylene production capacity of pollen grains is consistent with the expression of ethylene biosynthesis and signaling genes in these tissues [23]. Moreover, the application of high concentration of exogenous ethylene on male gametophyte induces the degradation of the generative cell at meiosis stage resulting in male sterility [24].…”

Section: Male Reproductive Organsmentioning

confidence: 69%

“…In petunia, the level of ACC increases 100 fold in mature pollen grain and in anthers one day before anthesis [46]. In tomato, it was shown that pollen grain has the capacity to produce and sense ethylene before germination, as suggested by the expression of several ethylene biosynthesis and signaling genes including SlAC S3, SlAC S11, SlETR3 and SlC TR2 [23]. Nevertheless, exogenous application of ACC in flowers cannot induce the post-pollination development [47].…”

Section: Pollen Germination and Tube Growthmentioning

confidence: 99%

Auxin and ethylene regulation of fruit set

Almasaud

Bouzayen

et al. 2020

Plant Science

View full text Add to dashboard Cite

With the forecasted fast increase in world population and global climate change, providing sufficient amounts of quality food becomes a major challenge for human society. Seed and fruit crop yield is determined by devel opmental processes including fl ower initiation, pollen fertility and fruit set. Fruit set is defined as the transition from flower to young fruit, a key step in the development of sexually reproducing higher plants. Plant hormones have important roles during fl ower pollination and fertilization, Jeading to fr uit set. Moreover, it is well es tablished that fruit set can be triggered by phytohormones like auxin and gibberellins (GAs), in the absence of fertilization, both hormones being cornmonly used to produce parthenocarpic fr uits and to increase fruit yield. Adclitionally, a number of stuclies highlighted the role of ethylene in plant reproductive organ development. The present review integrates current knowledge on the roles of auxin and ethylene in different steps of the fr uit set process with a specific emphasis on the interactions between the two hormones. A deeper understanding of the interplay between auxin and ethylene may provide new Jeads towards designing strategies for a better control of fmit initiation and ultimately yield.

show abstract

“…However, the phenotypic analyses performed on the 10 landraces allowed us to identify those that exhibited the best stable performances in terms of yield under a wide range of conditions, including high temperatures. Usually, heat tolerance has been evaluated under fixed climatic conditions in greenhouses or growth chambers [ 25 , 26 , 27 ]. However, these conditions do not reflect those observed in the fields during the tomato growing seasons.…”

Section: Discussionmentioning

confidence: 99%

High-Throughput Genotyping of Resilient Tomato Landraces to Detect Candidate Genes Involved in the Response to High Temperatures

Olivieri

Calafiore

Francesca

et al. 2020

Genes

View full text Add to dashboard Cite

The selection of tolerant varieties is a powerful strategy to ensure highly stable yield under elevated temperatures. In this paper, we report the phenotypic and genotypic characterization of 10 tomato landraces to identify the best performing under high temperatures. The phenotyping of five yield-related traits allowed us to select one genotype that exhibits highly stable yield performances in different environmental conditions. Moreover, a Genotyping-by-Sequencing approach allowed us to explore the genetic variability of the tested genotypes. The high and stable yielding landrace E42 was the most polymorphic one, with ~49% and ~47% private SNPs and InDels, respectively. The effect of 26,113 mutations on proteins’ structure was investigated and it was discovered that 37 had a high impact on the structure of 34 proteins of which some are putatively involved in responses to high temperatures. Additionally, 129 polymorphic sequences aligned against tomato wild species genomes revealed the presence in the genotype E42 of several introgressed regions deriving from S. pimpinellifolium. The position on the tomato map of genes affected by moderate and high impact mutations was also compared with that of known markers/QTLs (Quantitative Trait Loci) associated with reproductive and yield-related traits. The candidate genes/QTLs regulating heat tolerance in the selected landrace E42 could be further investigated to better understand the genetic mechanisms controlling traits for high and stable yield trait under high temperatures.

show abstract

Ethylene production and signaling in tomato (Solanum lycopersicum) pollen grains is responsive to heat stress conditions

Cited by 31 publications

References 59 publications

Proteomics of Heat-Stress and Ethylene-Mediated Thermotolerance Mechanisms in Tomato Pollen Grains

Proteomics of Heat-Stress and Ethylene-Mediated Thermotolerance Mechanisms in Tomato Pollen Grains

Auxin and ethylene regulation of fruit set

High-Throughput Genotyping of Resilient Tomato Landraces to Detect Candidate Genes Involved in the Response to High Temperatures

Contact Info

Product

Resources

About