Ethylene responsive transcription factor ERF109 retards PCD and improves salt tolerance in plant

Bahieldin, Ahmed; Atef, Ahmed; Edris, Sherif; Gadalla, Nour O.; Ali, Haytham A.; Hassan, Sabah M.; Al-Kordy, Magdy A.; Ramadan, Ahmed M.; Makki, Rania M.; Al-Hajar, Abdulrahman S. M.; El-Domyati, Fotouh M.

doi:10.1186/s12870-016-0908-z

Cited by 64 publications

(33 citation statements)

References 39 publications

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“…The AP2/ERF superfamily plays a pivotal role in adaptation to biotic stresses (72), emphasising that the innate immune response of the plant is highly active. This was confirmed by other highly induced ERFs , notably an ERF109 (VIT_03s0063g00460) , which plays an important role in abiotic stress adaptation, as reported for salt tolerance (73). Two other ERFs related to AP2 (VIT_18s0072g00260 and VIT_11s0016g00670) were simultaneously upregulated up to 50 times by the EO treatment.…”

Section: Resultssupporting

confidence: 70%

Oregano essential oil vapour preventsPlasmopora viticolainfection in grapevine (Vitis Vinifera) by triggering autoimmune metabolic pathways

Rienth

Crovadore

Ghaffari

et al. 2019

Preprint

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The reduction of synthetic fungicides in agriculture a major challenge in maintaining sustainable production, protecting the environment and consumers’ health. Downy mildew caused by the oomycete Plasmopora viticola is the major pathogen in viticulture worldwide and responsible for up to 60% of pesticide treatments. Alternatives to reduce fungicides are thus utterly needed to ensure sustainable vineyard-ecosystems, consumer health and public acceptance. Essential oils (EOs) are amongst the most promising natural plant protection alternatives and have shown their antibacterial, antiviral and antifungal properties on several agricultural crops. However, the efficiency of EOs highly depends on timing, application method and the molecular interactions between the host, the pathogen and EO. Despite proven EO efficiency, the underlying processes are still not understood and remain a black box. The objectives of the present study were: a) to evaluate whether a continuous fumigation of a particular EO can control downy mildew in order to circumvent the drawbacks of direct application, b) to decipher molecular mechanisms that could be triggered in the host and the pathogen by EO application and c) to try to differentiate whether essential oils directly repress the oomycete or act as plant resistance primers.A custom-made climatic chamber was used for a continuous fumigation of potted vines with different EOs during long-term experiments. The grapevine (Vitis vinifera) cv Chasselas was chosen in reason of its high susceptibility to Plasmopara viticola. Grapevine cuttings were infected with P. viticola. and subsequently exposed to continuous fumigation of different EOs at different concentrations, during 2 application time spans (24 hours and 10 days). Experiments were stopped when infection symptoms were clearly observed on the leaves of the control plants. Plant physiology (photosynthesis and growth rate parameters) were recorded and leaves were sampled at different time points for subsequent RNA extraction and transcriptomics analysis. Strikingly, the Oregano vulgare essential oil vapour treatment during 24h post-infection proved to be sufficient to reduce downy mildew development by 95%. Total RNA was extracted from leaves of 24h and 10d treatments and used for whole transcriptome shotgun sequencing (RNA-seq). Sequenced reads were then mapped onto the V. vinifera and P. viticola genomes. Less than 1% of reads could be mapped onto the P. viticola genome from treated samples, whereas up to 30 % reads from the controls mapped onto the P. viticola genome, thereby confirming the visual observation of P. viticola absence in the treated plants. On average, 80 % of reads could be mapped onto the V. vinifera genome for differential expression analysis, which yielded 4800 modulated genes. Transcriptomic data clearly showed that the treatment triggered the plant’s innate immune system with genes involved in salicylic, jasmonic acid and ethylene synthesis and signaling, activating Pathogenenesis-Related-proteins as well as phytoalexin synthesis.These results elucidate EO-host-pathogen interactions for the first time and indicate that the antifungal efficiency of EO is mainly due to the triggering of resistance pathways inside the host plants. This is of major importance for the production and research on biopesticides, plant stimulation products and for resistance-breeding strategiesAuthor SummaryThe reduction of synthetic plant protection products is a major concern of modern agriculture. The oomycetePlasmopora viticolawhich causes downy mildew in grapevine is amongst the most important grapevine pests and responsible for the dispersion of huge amounts of pesticides in vineyards. Among the evaluated alternatives to reduce or replace synthetic pesticides, plant volatile compounds could represent a sustainable solution. Some plant essential oils (EOs) have already shown antifungal capacities. However, their application is often difficult in terms of the right timing of treatment, degradation, bad rainfastness, mixability and phytotoxicity.The aim of the present work was to investigate whether the vapour phase, applied by a continuous fumigation of different EOs, might inhibit the development of downy mildew on grapevine, and in case of proven efficiency, to study the induced transcriptomic changes by RNA-sequencing in an attempt to elucidate the underlaying molecular interactions. Our results showed that the vapour phases ofO. vulgareandT. vulgariswere highly efficient against the pathogen. The study of differentially expressed genes indicated that the EO vapour triggered the main mechanisms of the plant’s innate immune system such as PTI (Pattern-Triggered Immunity) and ETI (Effector Triggered immunity).For the first time these results highlight the effects of EOs vapour on plant genes expression, which is very valuable information for the development of new natural plant protection products, as well as for breeding disease resistant cultivars.

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Section: Resultssupporting

confidence: 70%

Oregano essential oil vapour preventsPlasmopora viticolainfection in grapevine (Vitis Vinifera) by triggering autoimmune metabolic pathways

Rienth

Crovadore

Ghaffari

et al. 2019

Preprint

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“…Some other proteins, such as SCaBP8/CBL10 and 14-3-3 proteins, also play important roles in the SOS pathway (Quan et al 2007). Additionally, HKT proteins and the phytohormones abscisic acid (ABA), ethylene, auxin have been reported to be important regulators of plant salt responses (Ren et al 2005;Fernandez-Garcia et al 2011;Tognetti et al 2012;Duan et al 2013;Bahieldin et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Genome‐wide association study dissects the genetic bases of salt tolerance in maize seedlings

Luo

Wang

Gao

et al. 2019

JIPB

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Excess salinity is a natural stress that causes crop yield losses worldwide. The genetic bases of maize salt tolerance remain largely unknown. Here we investigated the survival rates of 445 maize natural accessions after salt treatments. A skewed distribution of the salt‐tolerant phenotypes was observed in this population. Genome‐wide association studies (GWAS) revealed 57 loci significantly associated with salt tolerance. Forty‐nine candidate genes were detected from these loci. About 10% of these genes were co‐localized with loci from QTL mapping. Forty four percent of the candidate genes were involved in stress responses, ABA signaling, stomata division, DNA binding/transcription regulation and auxin signaling, suggesting that they are key genetic mechanisms of maize salt tolerance. Transgenic studies showed that two genes, the salt‐tolerance‐associated‐gene 4 (SAG4, GRMZM2G077295) and SAG6 (GRMZM2G106056), which encode a protein transport protein and the double‐strand break repair protein MRE11, respectively, had positive roles in plant salt tolerance, and their salt‐tolerant haplotypes were revealed. The genes we identified in this study provide a list of candidate targets for further study of maize salt tolerance, and of genetic markers and materials that may be used for breeding salt‐tolerance in maize.

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“…For example, a rice AP2/ERF type TF, SERF1 (SALT-RESPONSIVE ERF1), responds to salt stress and participates in regulation of grain filling and gibberellin-meditated seedling establishment (Schmidt et al, 2014). Some AP2/ERF proteins, like GmRAV-03, JcERF011, and ERF109 are involved in the regulation of salt tolerance in plants (Bahieldin et al, 2016;Tang et al, 2016b;Zhao et al, 2017). Rice INDETERMINATE SPIKELET1 (IDS1), a typical AP2 type TF, has been reported to play roles in inflorescence architecture and the establishment of floral meristems (Lee and An, 2012).…”

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confidence: 99%

INDETERMINATE SPIKELET1 Recruits Histone Deacetylase and a Transcriptional Repression Complex to Regulate Rice Salt Tolerance

et al. 2018

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Perception and transduction of salt stress signals are critical for plant survival, growth, and propagation. Thus, identification of components of the salt stress-signaling pathway is important for rice () molecular breeding of salt stress resistance. Here, we report the identification of an apetala2/ethylene response factor transcription factor INDETERMINATE SPIKELET1 (IDS1) and its roles in the regulation of rice salt tolerance. By genetic screening and phenotype analysis, we demonstrated that IDS1 conferred transcriptional repression activity and acted as a negative regulator of salt tolerance in rice. To identify potential downstream target genes regulated by IDS1, we conducted chromatin immunoprecipitation (ChIP) sequencing and ChIP-quantitative PCR assays and found that IDS1 may directly associate with the GCC-box-containing motifs in the promoter regions of abiotic stress-responsive genes, including () and (), which are key genes regulating rice salt tolerance. IDS1 physically interacted with the transcriptional corepressor topless-related 1 and the histone deacetylase HDA1, contributing to the repression of and expression. Analyses of histone H3 acetylation status and RNA polymerase II occupation on the promoters of and further defined the molecular foundation of the transcriptional repression activity of IDS1. Our findings illustrate an epigenetic mechanism by which IDS1 modulates salt stress signaling as well as salt tolerance in rice.

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Ethylene responsive transcription factor ERF109 retards PCD and improves salt tolerance in plant

Cited by 64 publications

References 39 publications

Oregano essential oil vapour preventsPlasmopora viticolainfection in grapevine (Vitis Vinifera) by triggering autoimmune metabolic pathways

Oregano essential oil vapour preventsPlasmopora viticolainfection in grapevine (Vitis Vinifera) by triggering autoimmune metabolic pathways

Genome‐wide association study dissects the genetic bases of salt tolerance in maize seedlings

INDETERMINATE SPIKELET1 Recruits Histone Deacetylase and a Transcriptional Repression Complex to Regulate Rice Salt Tolerance

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