INDETERMINATE SPIKELET1 Recruits Histone Deacetylase and a Transcriptional Repression Complex to Regulate Rice Salt Tolerance

Abstract

Perception and transduction of salt stress signals are critical for plant survival, growth, and propagation. Thus, identification of components of the salt stress-signaling pathway is important for rice () molecular breeding of salt stress resistance. Here, we report the identification of an apetala2/ethylene response factor transcription factor INDETERMINATE SPIKELET1 (IDS1) and its roles in the regulation of rice salt tolerance. By genetic screening and phenotype analysis, we demonstrated that IDS1 conferred transcriptional repression activity and acted as a negative regulator of salt tolerance in rice. To identify potential downstream target genes regulated by IDS1, we conducted chromatin immunoprecipitation (ChIP) sequencing and ChIP-quantitative PCR assays and found that IDS1 may directly associate with the GCC-box-containing motifs in the promoter regions of abiotic stress-responsive genes, including () and (), which are key genes regulating rice salt tolerance. IDS1 physically interacted with the transcriptional corepressor topless-related 1 and the histone deacetylase HDA1, contributing to the repression of and expression. Analyses of histone H3 acetylation status and RNA polymerase II occupation on the promoters of and further defined the molecular foundation of the transcriptional repression activity of IDS1. Our findings illustrate an epigenetic mechanism by which IDS1 modulates salt stress signaling as well as salt tolerance in rice.

Comprehensive analysis of Q gene near‐isogenic lines reveals key molecular pathways for wheat domestication and improvement

“…Two rice AP2 genes, supernumerary bract ( SNB ) and OsIDS1 , synergistically control inflorescence architecture and floral meristem establishment (Lee and An, ). SNB and OsIDS1 also regulate lodicule development and rice salt tolerance (Cheng et al , ). A most recent study also showed that SNB controls rice seed shattering and seed size (Jiang et al , ).…”

Histone Acetylation Dynamics Integrates Metabolic Activity to Regulate Plant Response to Stress

“…Site specificity of HDACs on histones has not been clearly demonstrated. Alteration of rice HDT701 expression level affects global H4K5 and H4K16 acetylation levels, while many HDACs regulates H3 acetylation at specific genes (Ding et al, 2012; Zheng et al, 2016; Liu et al, 2017; Ueda et al, 2017; Wang et al, 2017; Cheng et al, 2018; Park et al, 2018). In addition to histones, non-histone substrates of plant RPD3/HDA1 family HDACs has also been identified (Hartl et al, 2017).…”

Genome-Wide Identification of Histone Acetyltransferases and Deacetylases in Epiphytic Orchid Plant Dendrobium Catenatum

“…overexpression of HDA702 enhances growth rate, alters plant architecture [64], and increases root growth through deacetylating OsNAC6 locus [65]. HDA702 implicates in formation of IDS1-TPR1-HDA1 transcriptional repression complex, contributing to negative regulation of salt tolerance [66]. These data suggest that HDA702/703/710 in rice may have divergent developmental functions compared with closely related homologs in Arabidopsis.…”