Ethylenediaminetetraacetic acid (EDTA) does not increase iron uptake or ferritin synthesis by Caco-2 cells

Carotenoids, but Not Vitamin A, Improve Iron Uptake and Ferritin Synthesis by Caco‐2 Cells from Ferrous Fumarate and NaFe‐EDTA

“…Another intriguing result is the low uptake of iron by Caco‐2 cells in presence of NaFe‐EDTA. It has been reported previously with different EDTA salts (García‐Casal and others ) and was confirmed in this study. Caco‐2 cells seem unable to use iron from NaFe‐EDTA when administered alone, but respond to carotenoids increasing uptake and ferritin synthesis and to tannic acid decreasing both parameters, similar to ferrous fumarate.…”

“…The objectives of this work were: (1) to study iron uptake by Caco‐2 cells in presence of different concentrations of vitamin A and tannic acid, to evaluate the effect of a molar excess of vitamin A on iron uptake and on the inhibition produced by tannic acid; (2) to evaluate iron uptake and ferritin synthesis in presence of carotenoids without pro‐vitamin A activity: lycopene, lutein, and zeaxantin (lycopene, an acyclic carotenoid, was also used to evaluate if structural differences could have a role on iron uptake); (3) due to the previously reported low uptake of iron from sodium–iron ethylenediamine tetraacetic acid (NaFe‐EDTA) by Caco‐2 cells as well as its inability to promote ferritin synthesis (García‐Casal and others ), all experiments with vitamin A and carotenoids were performed comparing iron uptake and ferritin synthesis from ferrous fumarate and NaFe–EDTA.…”

Nutriomic Analysis of Fresh and Processed Fruit Products. 2. During in Vitro Simultaneous Molecular Passages Using Caco-2 Cell Monolayers

“…Intensive studies on the use of Caco-2 cell monolayers as a mimetic model for human absorptive tissues suggest that the cell line is applicable for a range of target nutriome components: carotenoids − , phenolics − , minerals (e.g., iron) and affecting factors (e.g., organic acids, ascorbic acid, and antinutrition phytic acid) − , and sugar transporters . Because enterocytes are living cells, they can also metabolize and transport nutrients.…”