Etidronic acid and ethylenediaminetetraacetic acid associated with sodium hypochlorite have limited effect on the compressive fracture resistance of roots ex vivo

Abstract

Aims: To assess the effect of etidronic acid (EA) mixed with sodium hypochlorite (NaOCl) and two ethylenediaminetetraacetic acid-containing preparations (EDTA and SmearClear) alternated with NaOCl, as a final rinse, on root fracture resistance to a compressive force. Materials and Methods: Seventy-two premolar roots were randomly allocated to the following groups according to the irrigant solutions. For the EA group, 9% EA + 2.5% NaOCl were used throughout the assays ( n = 21). The remaining groups received 2.5% NaOCl during and immediately after chemo-mechanical instrumentation. Intermediately, the roots received 17% EDTA ( n = 19), or SmearClear ( n = 16), or no irrigation (control) ( n = 16) plus a final rinse with 2.5% NaOCl. The specimens were subjected to a vertical compressive force loaded at a crosshead speed of 0.02 mm/s parallel to the long axis of the root until fracture occurred. The results were compared statistically using the one-way analysis of variance for intergroup comparisons. Results: The negative control presented with the highest values, whereas the SmearClear presented with the lowest values, though no significant differences were found when comparing the different groups ( P = 0.82). Conclusions: The use of EA + NaOCl or two EDTA formulations in association with NaOCl does not affect the fracture resistance of previously chemo-mechanically prepared roots.

2D and 3D Erosion Landscape Analysis of Endodontic-Treated Teeth Using EDTA and HEDP as Chelating Agents: A High-Resolution Micro-Computed Tomographic Study

“…The duration of irrigations is an additional factor that could influence the strength and fracture resistance of treated roots, as highlighted in previous studies [39,54,55]. In the current investigation, this variable was not taken into account, preventing a direct comparison between our results and those reported in prior studies.…”

“…Methodologically, the majority of these studies have employed SEM and light microscopy to examine the eroded surface of root canal dentine walls [3,[35][36][37]. Additionally, some studies have used fractured specimens to measure the actual depth of erosion [38,39]. However, these methods have their limitations.…”

Ex vivo study of the effect of different antimicrobial solutions on apical extra-radicular biofilm: A CLSM study

“…It inhibits matrix metalloproteinase enzyme, enhances periodontal healing in replanted avulsed teeth and periodontal therapies (4) . It has a low acidic pH (2.2); therefore, it chelates with calcium ions with mild demineralization effect which enhances connective tissue healing (5) Etidronic acid (1-hydroxyethane 1,1-diphosphonic acid (HEDP) is a weak chelator that that has the advantage of preserving the antimicrobial and proteolytic action of sodium hypochlorite, thus it was recommended to use this mixture for root canal irrigation for disinfection of the root canal system and smear layer removal throughout the preparation procedure instead of a final rinse (6,7) . Silver citrate nanoparticles is recently used as an endodontic irrigant for the effective antibacterial properties of silver nanoparticles combined with the chelating action of citrate rendering this combination an anti-biofilm and smear layer removal irrigant (8,9) .…”