Etoposide-induced protein 2.4 ameliorates high glucose-induced epithelial-mesenchymal transition by activating adenosine monophosphate-activated protein kinase pathway in renal tubular cells

Li, Fan; Guo, Dongwei; Zhi, Shufeng; Jia, Keqi; Wang, Yuxue; Zhang, Aobo; Pei, Yuqi; Hao, Jun

doi:10.1016/j.biocel.2021.106117

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…EI24 (etoposide-inducible 2.4 protein) is closely associated with the activation of the adenosine monophosphate-activated kinase (AMPK)/mammalian target of rapamycin (mTOR) axis and can promote the degradation of autophagic lysosomes, thus exerting a protective effect on autophagy 30 . Accordingly, we examined the upstream of AMPK regulation and EI24 levels in liver tissue or hepatocytes.…”

Section: Resultsmentioning

confidence: 99%

Comparison of effects of HucMSCs, exosomes, and conditioned medium on NASH

Liang,

Gao,

Gao

et al. 2023

Sci Rep

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To investigate the effects and potential mechanisms of human umbilical cord mesenchymal stem cells, exosomes, and their conditioned media on lipid storage in oleic acid (OA) and palmitic acid (PA) treated hepatocytes and high-fat methionine- choline deficient diet (HFMRCD) induced non-alcoholic steatohepatitis (NASH) mice. AML12 cells were stimulated with OA and PA to establish the lipid storage cell model. HucMSCs, exosomes, and culture medium were then co-cultured. At the same time, C57BL/6 mice were fed an HFMRCD for 6 or 8 weeks to establish a NASH mouse model. The effect of HucMSCs, exosomes, and culture medium on lipid droplet repair of hepatocytes or NASH mice was then assessed. The weight of hepatocytes or liver tissue, Oil Red O, hematoxylin–eosin staining, Masson staining, Western blot, and qPCR were used to detect the related IL-6, TNF-α, TGF-β1 andEI24/AMPK/mTOR pathway expression in hepatocytes and liver tissue. Compared with the model group, the effect of HucMSCs-Ex on inhibiting the accumulation of lipid droplets was more obvious at the cell level. In vivo study showed that HucMSCs-Ex reduces activity scores in NASH mice and improves liver tissue morphology by reducing vacuolar degeneration, fat deposition, and collagen deposition of liver tissue. Western blot and qPCR results showed that inflammatory factors and AMPK/mTOR or EI24-related autophagy pathways were altered before and after treatment. HucMSCs, HucMSC-Ex, and CM can promote autophagy in hepatocytes or NASH mice through the AMPK/mTOR or EI24-related autophagy pathway and alleviate injury associated with lipid deposition, collagen deposition or inflammation, reversing the progression of NASH.

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Section: Resultsmentioning

confidence: 99%

Comparison of effects of HucMSCs, exosomes, and conditioned medium on NASH

Liang,

Gao,

Gao

et al. 2023

Sci Rep

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“…Natural polyphenols can reverse epigenetic alterations of autophagy and delay the progression of DKD ( 119 ). Resveratrol was found to regulate SIRT1 and DNA-methyltransferase (DNMT) and exhibited potential regulatory capacity on DKD ( 120 , 121 ). Similarly, quercetin has also been found to regulate the expression of multiple chromatin modifiers (including DNMTs, histone deacetylases, histone acetyltransferases, and histone methyltransferases) ( 122 ).…”

Section: Polyphenols Used To Regulate Autophagy In Dkdmentioning

confidence: 99%

Regulation of autophagy by natural polyphenols in the treatment of diabetic kidney disease: therapeutic potential and mechanism

Liu,

Jin,

Yang

et al. 2023

Front. Endocrinol.

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Diabetic kidney disease (DKD) is a major microvascular complication of diabetes and a leading cause of end-stage renal disease worldwide. Autophagy plays an important role in maintaining cellular homeostasis in renal physiology. In DKD, the accumulation of advanced glycation end products induces decreased renal autophagy-related protein expression and transcription factor EB (TFEB) nuclear transfer, leading to impaired autophagy and lysosomal function and blockage of autophagic flux. This accelerates renal resident cell injury and apoptosis, mediates macrophage infiltration and phenotypic changes, ultimately leading to aggravated proteinuria and fibrosis in DKD. Natural polyphenols show promise in treating DKD by regulating autophagy and promoting nuclear transfer of TFEB and lysosomal repair. This review summarizes the characteristics of autophagy in DKD, and the potential application and mechanisms of some known natural polyphenols as autophagy regulators in DKD, with the goal of contributing to a deeper understanding of natural polyphenol mechanisms in the treatment of DKD and promoting the development of their applications. Finally, we point out the limitations of polyphenols in current DKD research and provide an outlook for their future research.

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Apigenin inhibits migration and induces apoptosis of human endometrial carcinoma Ishikawa cells via PI3K-AKT-GSK-3β pathway and endoplasmic reticulum stress

Liang

Zhong

et al. 2022

Journal of Functional Foods

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Etoposide-induced protein 2.4 ameliorates high glucose-induced epithelial-mesenchymal transition by activating adenosine monophosphate-activated protein kinase pathway in renal tubular cells

Cited by 3 publications

References 30 publications

Comparison of effects of HucMSCs, exosomes, and conditioned medium on NASH

Comparison of effects of HucMSCs, exosomes, and conditioned medium on NASH

Regulation of autophagy by natural polyphenols in the treatment of diabetic kidney disease: therapeutic potential and mechanism

Apigenin inhibits migration and induces apoptosis of human endometrial carcinoma Ishikawa cells via PI3K-AKT-GSK-3β pathway and endoplasmic reticulum stress

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