Eubacterium acidaminophilum sp. nov., a versatile amino acid-degrading anaerobe producing or utilizing H2 or formate

Zindel, U.; Freudenberg, W.; Rieth, M.; Andreesen, Jan R.; Schnell, Jessica K.; Widdel, Friedrich

doi:10.1007/bf00407789

Cited by 170 publications

(154 citation statements)

References 58 publications

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“…The classic mode of amino acid fermentation is the Strickland reaction in which a pair of amino acids is consumed, one acting as an oxidant and the other as a reductant. For example, a single bacterium can oxidize alanine to acetate and NKt while reducing glycine to acetate and NH4": (Zindel et al, 1988). However, H 2 and acetate generated from alanine oxidation can be consumed by other partners such as methanogens (Nagase and Matsuo, 1982), SO4 -reducers (Nanninga and Gottschal, 1985), or acetogens (Zindel et al, 1988).…”

Section: Reactionmentioning

confidence: 99%

“…For example, a single bacterium can oxidize alanine to acetate and NKt while reducing glycine to acetate and NH4": (Zindel et al, 1988). However, H 2 and acetate generated from alanine oxidation can be consumed by other partners such as methanogens (Nagase and Matsuo, 1982), SO4 -reducers (Nanninga and Gottschal, 1985), or acetogens (Zindel et al, 1988). The Strickland reaction is energetically favorable over the syntrophic fermentation of amino acids (Schink, 1997) and the former may dominate in habitats rich in amino acids.…”

Section: Reactionmentioning

confidence: 99%

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Anaerobic Metabolism: Linkages to Trace Gases and Aerobic Processes

Megonigal¹,

Hines²,

Visscher³

2014

Treatise on Geochemistry

128

164

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Section: Reactionmentioning

confidence: 99%

Section: Reactionmentioning

confidence: 99%

Anaerobic Metabolism: Linkages to Trace Gases and Aerobic Processes

Megonigal¹,

Hines²,

Visscher³

2014

Treatise on Geochemistry

128

164

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“…In some cases, the difficult ox-idation processes can be coupled efficiently with, e.g., glycine reduction in the same organism as exemplified by the stickland reaction. However, this view is rather narrow as the versatile metabolism of Eubacterium acidaminophilum (Zindel et al 1988) shows. This bacterium can either combine the oxidative and the reductive part of the Stickland fermentation simultaneously, or run either one of both separate, either with a hydrogen-consuming partner organism or with hydrogen as external electron donor.…”

Section: Syntrophic Associations In Methanogenic Cooperationmentioning

confidence: 99%

Untitled

Schink

2002

Antonie Van Leeuwenhoek

259

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After several decades of microbiological research has focused on pure cultures, synergistic effects between different types of microorganisms find increasing interest. Interspecies interactions between prokaryotic cells have been studied into depth mainly with respect to syntrophic cooperations involved in methanogenic degradation of electron-rich substrates such as fatty acids, alcohols, and aromatics. Partners involved in these processes have to run their metabolism at minimal energy increments, with only fractions of an ATP unit synthesized per substrate molecule metabolized, and their cooperation is intensified by close proximity of the partner cells. New examples of such syntrophic activities are anaerobic methane oxidation by presumably methanogenic and sulfate-reducing prokaryotes, and microbially mediated pyrite formation. Syntrophic relationships have also been discovered to be involved in the anaerobic metabolization of amino acids and sugars where energetical restrictions do not necessarily force the partner organisms into strict interdependencies. The most highly developed cooperative systems among prokaryotic cells appear to be the structurally organized phototrophic consortia of the Chlorochromatium and Pelochromatium type in which phototrophic and chemotrophic bacteria not only exchange metabolites but also interact at the level of growth coordination and tactic behaviour.

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“…Strains KRE 4T and KRE 2 were initially characterized by using a variety of standard physiological tests directed to the Eubacterium speciescomplex (Andreesen, 1992;Zindel et al, 1988) and were found to be similar to T. creatinini, strain BN 1 IT described by Gauglitz (1988). Key enzymes, such as glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, glutamate-and malate dehydrogenases, from strain KRE 4T were determined according to the methods described by Bergmeyer (1983).…”

Section: Isolation and Cultivation Of Creatinine-degrading Strainsmentioning

confidence: 99%

“…Clostridium paraputrijicum and Clostridium perfringens metabolized creatinine and creatine anaerobically to N-methylhydantoin (Szulmajster, 1958a, b;ten Krooden & Owens, 1957). The creatine-and sarcosine-utilizing Eubacterium acidaminophilum was shown to require selenite and suitable electron donors (Hormann & Andreesen, 1989 ;Zindel et al, 1988). Creatinine and N-methylhydantoin degradation was studied in several species of Clostridium, Eubacterium and Peptostreptococcus (Gauglitz, 1988).…”

Section: Introductionmentioning

confidence: 99%

Tissierella creatinophila sp. nov., a Gram-positive, anaerobic, non-spore-forming, creatinine-fermenting organism

Harms

Schleicher

Collins³

et al. 1998

International Journal of Systematic Bacteriology

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A strictly anaerobic, Gram-positive, non-spore-forming bacterium was isolated from sewage sludge which grew on creatinine as sole source of carbon and energy. This new isolate, designated strain KRE 4l, totally degraded creatinine via creatine, sarcosine and glycine to the products acetate, monomethylamine, ammonia and carbon dioxide. Growth on creatinine or creatine was seleniumdependent and stimulated by formate, indicating the involvement of a creatine reductase, sarcosine reductase and/or glycine reductase. This was substantiated by the fact that creatine, sarcosine and glycine were reduced by cell-free extracts. Growth on creatinine or creatine was also possible in the absence of formate, but with an increase in doubling time. The new bacterium occurred as rod-shaped cells, which exhibited an angular form (2-6 pm long and 0.7-1.1 pm wide) and showed motility by means of peritrichous flagella. The G+C content of the DNA was 30 mol0/o. Comparative 16s rRNA sequence analysis demonstrated that strain KRE 4T represents a new subline within the genus Tissierella. Due to its very restricted substrate spectrum and the inability of whole cells to utilize sarcosine and glycine as intermediates of creatine breakdown, this organism can be readily separated from currently described species of Tissierella. Therefore, based on the phenotypic and phylogenetic distinctiveness of the new isolate, it is proposed that the bacterium be classified as a new species of the genus Tissierella, Tissierella creatinophila sp. nov. The type strain is KRE 4 (= DSM 69113.

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Eubacterium acidaminophilum sp. nov., a versatile amino acid-degrading anaerobe producing or utilizing H2 or formate

Cited by 170 publications

References 58 publications

Anaerobic Metabolism: Linkages to Trace Gases and Aerobic Processes

Anaerobic Metabolism: Linkages to Trace Gases and Aerobic Processes

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Tissierella creatinophila sp. nov., a Gram-positive, anaerobic, non-spore-forming, creatinine-fermenting organism

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