Eucalyptus nitens plant regeneration from seedling explants through direct adventitious shoot bud formation

Ayala, Paula Gabriela; Brugnoli, Elsa Andrea; Luna, Claudia Verónica; González, Ana María; Pezzutti, Raúl Vicente; Sansberro, Pedro Alfonso

doi:10.1007/s00468-019-01888-5

Cited by 29 publications

(13 citation statements)

References 20 publications

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“…The formation of adventitious shoots is a complex process that involves the participation of PGRs, mainly auxins and CKs, with multiple signaling pathways (Sang et al 2018;Tian et al 2018;Ikeuchi et al 2019). Compared with auxins, CKs are involved in bud breakage, cell division, shoot initiation, and multiplication from explants, which are more frequently applied to induce in vitro shoot organogenesis (Mazri 2015;Zhang et al 2018;Ayala et al 2019). The exogenous CKs application facilitates the explant to reach an optimum cytokinin: auxin ratio for de novo meristem formation (Alvarez et al 2020).…”

Section: De Novo Shoot Organogenesis From Korean Pine Sprouted Seeds Explantsmentioning

confidence: 99%

Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Liang

Bai

et al. 2021

Plant Cell Tiss Organ Cult

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The de novo organogenesis system for Korean pine of this economically and ecologically coniferous species was successfully established using sprouted seeds as the original explants. After 30 days of incubation, 92.67% of explants produced direct shoots on Gupta and Durzan(DCR) medium containing 2 mg • L −1 kinetin (KT) in combination 0.5 mg • L −1 thidiazuron (TDZ) with a maximum of about 15 shoots per explant respectively. We also confirmed the organogenic regeneration pattern by scanning electron microscopy (SEM) observation. For shoot elongation and growth after 60 days of culture, we obtained the highest mean length of 34.99 mm from DCR basal media supplemented with 6-benzyladenine (6-BA; 0.2 mg • L −1 ), 1-Naphthaleneacetic acid (NAA; 0.1 mg • L −1 ), and activated charcoal (AC; 1 g • L −1 ). The highest rooting percentages of 20.74-21.48% were observed within two months in the 1/2 DCR medium (major elements halved) enriched with 0.05 mg•L −1 NAA and 0.5 or 1 mg • L −1 indole-3-butyric acid (IBA).Rooted plants showed a survival rate of 90.28% in perlite: peat: vermiculite = 1:1:1 after acclimatization. This protocol is a successful and efficient biotechnological approach to the micropropagation of Korean pine, and these data will be helpful to the clonal propagation and conservation of Korean pine.

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Section: De Novo Shoot Organogenesis From Korean Pine Sprouted Seeds Explantsmentioning

confidence: 99%

Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Liang

Bai

et al. 2021

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

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“…Indeed, eucalyptus shoots cultured in a two-flask system subjected to PAM (without sugar supplementation and with CO 2 enriched air; Figure 6), showed higher photosynthetic rates and epicuticular leaf-wax contents (as well as better stomatal function) than shoots cultured in conventional SS [61]. Moreover, gas exchange did not only favor the development of the aerial part of the plant, as rooting percentages and/or root quality were also improved by the use of TIS during the rooting phase [60,62,63]. The supply of O 2 in the rooting zone promoted the development of normal roots directly from the base of the stems, without callus interference [60].…”

Section: Axillary Shoots Cultured By Temporary Immersionmentioning

confidence: 99%

“…These problems have been addressed by the use of different TIS with forced ventilation, such as RITA ® and various designs of the two‐flask system (Figures and ). Bioreactors were used either for multiplication and rooting or only for the phase of rooting . As reported by McAlister et al., great differences among clones regarding proliferation, rooting, and acclimation were detected .…”

Section: Application Of Bioreactors To the Micropropagation Of Treesmentioning

confidence: 99%

“…The supply of O 2 in the rooting zone promoted the development of normal roots directly from the base of the stems, without callus interference . The formation of well‐developed roots enhances the nutrient/water uptake rate of plants during the acclimation process, which was easier in eucalypt plants cultured by TIS than in those cultured in SS .…”

Section: Application Of Bioreactors To the Micropropagation Of Treesmentioning

confidence: 99%

“…As mentioned above, these trees are recalcitrant to vegetative propagation, mainly due to difficulties during rooting and acclimation. These problems have been addressed by the use of different TIS with forced ventilation, such as RITA ® and various designs of the two‐flask system (Figures and ). Bioreactors were used either for multiplication and rooting or only for the phase of rooting .…”

Section: Application Of Bioreactors To the Micropropagation Of Treesmentioning

confidence: 99%

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Use of bioreactor systems in the propagation of forest trees

Vidal

Sánchez

2019

Engineering in Life Sciences

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Plant biotechnology can be used to conserve the germplasm of natural forests, and to increase the productivity and sustainability of plantations. Both goals imply working with mature trees, which are often recalcitrant to micropropagation. Conventional in vitro culture uses closed containers and gelled medium with sugar supplementation. Bioreactor culture uses liquid medium and usually incorporates aeration. The increased absorption of nutrients via the liquid medium together with the renewal of the air inside the bioreactors may improve the physiological state of the explants. In this review, we will explore the feasibility of using bioreactors to overcome the recalcitrance of many trees to micropropagation and/or to decrease the cost of large‐scale propagation. We will focus on the recent use of bioreactors during the multiplication, rooting (plant conversion in the case of somatic embryos), and acclimation stages of the micropropagation of axillary shoots and somatic embryos of forest trees (including some shrubs of commercial interest), in both temporary and continuous immersion systems. We will discuss the advantages and the main obstacles limiting the widespread implementation of bioreactor systems in woody plant culture, considering published scientific reports and contributions from the business sector.

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Conventional Versus Non-Conventional Methods of Propagation of Forest Tree Species: Applications and Limitations

Manohar,

Shukla,

Shahina

et al. 2024

Biotechnological Approaches for Sustaining Forest Trees and Their Products

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Eucalyptus nitens plant regeneration from seedling explants through direct adventitious shoot bud formation

Cited by 29 publications

References 20 publications

Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Use of bioreactor systems in the propagation of forest trees

Conventional Versus Non-Conventional Methods of Propagation of Forest Tree Species: Applications and Limitations

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