Eupatilin Inhibits Renal Cancer Growth by Downregulating MicroRNA-21 through the Activation of YAP1

Abstract

Renal cell carcinoma (RCC) is the second most common human urinary tumor. Eupatilin is the main active ingredient of the traditional Chinese medicine Artemisia asiatica. The effect of Eupatilin on RCC and the underlying mechanism remain unknown. Here, we investigated the anticancer effects and mechanisms of Eupatilin in RCC in vivo and in vitro, laying an experimental foundation for the clinical application of Eupatilin in the treatment of RCC. The results showed that Eupatilin significantly inhibited 786-O cell viability and migration and promoted apoptosis. Eupatilin inhibited the expression of miR-21 in 786-O cells, and overexpression of miR-21 suppressed the effect of Eupatilin on viability, apoptosis, and migration in 786-O cells. Eupatilin inhibited the growth of renal tumors in nude mice by downregulating miR-21. YAP1, which was identified as a target of miR-21, showed significantly lower expression in RCC tissues than in healthy tissues. miR-21 significantly inhibited YAP1 protein expression in 786-O cells and tumor tissues isolated from nude mice, and YAP1 attenuated the effect of miR-21 on the viability, apoptosis, and migration of 786-O cells. In conclusion, Eupatilin inhibited the expression of miR-21, which mediated the proapoptotic and antimigratory effects of Eupatilin by suppressing YAP1 in renal cancer cells. These results suggested that Eupatilin could be a potent agent for the treatment of RCC.

Effects of Angelica Fritillaria Kushen Pill on Renal Function and Immune Function after Laparoscopic Radical Nephrectomy for Patients with Renal Carcinoma

“…Tan et al [21] found that the incidence of side effects in places such as cardiovascular system and digestive system in patients treated with traditional Chinese medicine was significantly lower than that of the single western medicine treatment when patients were treated after radical resection of RCC with TCM on the basis of basic western medicine treatment. Zhong et al [22] found that combined Chinese medicine therapy was more stable for patients with renal cancer after laparoscopic radical resection and prolonged survival time. In this study, Danggui Beimu Kushen Pills combined with interferon were used to treat patients after RCC.…”

Eupatilin Promotes Cell Death by Calcium Influx through ER-Mitochondria Axis with SERPINB11 Inhibition in Epithelial Ovarian Cancer

“…Treatment with eupatilin in the xenograft mouse model for esophageal cancer inhibits the activity of AKT and ERK1/2 in tissues [9]. Moreover, a recent study showed that eupatilin can target microRNAs in cancer cells to induce apoptosis, so further studies are needed to determine if eupatilin may have anticancer effects by modulating microRNAs in ovarian cancer cells [28]. Accumulated evidence suggests that eupatilin inhibits cell proliferation and induces apoptosis as a common mechanism in various cancer cells.…”

Aquilaria sinensis leaf tea affects the immune system and increases sleep in zebrafish

“…Research has shown that quercetin and quercetin 3-O-glucuronide regulate the sleep-wake cycle by activating the GABA(A) receptor and improving sleep duration and quality of insomnia mice ( Kambe et al, 2010 ; Kim et al, 2021 ). Eupatilin has been shown to inhibit pancreatic cancer cells, the growth of kidney cancer cells, and the metastasis of prostate cancer cells while improving the immune system ( Zhong et al, 2019 ; Serttas et al, 2021 ; Park and Kim, 2022 ). Our study first reports that eupatilin may improve sleep of a sleep-disturbed zebrafish, but it requires further investigation.…”