This article presents a new comprehensive assessment of the Holocene hydrological variability of Lake Ladoga, northwest Russia. The reconstruction is based on oxygen isotopes of lacustrine diatom silica (d 18 O diatom ) preserved in sediment core Co 1309, and is complemented by a diatom assemblage analysis and a survey of modern isotope hydrology. The data indicate that Lake Ladoga has existed as a freshwater reservoir since at least 10.8 cal. ka BP. The d 18 O diatom values range from +29.8 to +35.0&, and relatively higher d 18 O diatom values around +34.7& between c. 7.1 and 5.7 cal. ka BP are considered to reflect the Holocene Thermal Maximum. A continuous depletion in d 18 O diatom since c. 6.1 cal. ka BP accelerates after c. 4 cal. ka BP, indicating Middle to Late Holocene cooling that culminates during the interval 0.8-0.2 cal. ka BP, corresponding to the Little Ice Age. Lake-level rises result in lower d 18 O diatom values, whereas lower lake levels cause higher d 18 O diatom values. The diatom isotope record gives an indication for a rather early opening of the Neva River outflow at c. 4.4-4.0 cal. ka BP. Generally, overall high d 18 O diatom values around +33.5& characterize a persistent evaporative lake system throughout the Holocene. As the Lake Ladoga d 18 O diatom record is roughly in line with the 60°N summer insolation, a linkage to broader-scale climate change is likely.