Europium-doped bioapatite: a new photostable biological probe, internalizable by human cells

Doat, Anne; Fanjul, M; Pellé, F.; Hollande, E; Lebugle, A.

doi:10.1016/s0142-9612(03)00169-8

Cited by 150 publications

(93 citation statements)

References 15 publications

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“…The LD 50 measured in rats in vivo was reached at 550 mg/kg 52 . Eu is used as fluorescent marker designed for the cellular uptake and was tested in different cell types for this purpose without inducing major alterations [53][54][55] .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of short-term effects of rare earth and other elements used in magnesium alloys on primary cells and cell lines☆

Feyerabend¹,

Fischer²,

Holtz³

et al. 2010

Acta Biomaterialia

532

263

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Degradable magnesium alloys for biomedical application are on the verge of being used clinically. Rare earth elements (REE) are used to improve the mechanical properties of the alloys, however in more or less undefined mixtures. Therefore in this study the in vitro cytotoxicity of the elements yttrium (Y), neodymium (Nd), dysprosium (Dy), praseodymium (Pr), gadolinium (Gd), lanthanum (La), cerium (Ce), europium (Eu), lithium (Li), zirconium (Zr), was evaluated by incubation with the chlorides (10-2000 µM), magnesium (Mg) and calcium (Ca) were tested at higher concentrations (200 and 50 mM, respectively). The influence on viability of human osteosarcoma cell line MG-63, human umbilical cord perivascular (HUCPV) cells and mouse macrophages (RAW 264.7) was determined, as well as the induction of apoptosis and the expression of inflammatory factors (TNF-α, IL-1α). Significant differences between the applied cells could be observed. RAW exhibited the highest and HUCPV the lowest sensitivity. La and Ce showed the highest cytotoxicity of the analysed elements. From the elements with high solubility in magnesium alloys Gd and Dy seem to be more suitable than Y. The focus of magnesium alloy development for biomedical applications should include most defined alloy compositions with well known tissue specific and systemic effects.

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Section: Discussionmentioning

confidence: 99%

Evaluation of short-term effects of rare earth and other elements used in magnesium alloys on primary cells and cell lines☆

Feyerabend¹,

Fischer²,

Holtz³

et al. 2010

Acta Biomaterialia

532

263

View full text Add to dashboard Cite

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“…Another study [43], on non-colloidal apatite, showed the possibility to visualize by laser scanning confocal microscopy some Eu-doped apatite particles internalized by human pancreatic cells Capan-1, exploiting their luminescence feature. The development of colloidal formulations, allowing a control of particle size and size distribution, is now expected to widen considerably the significance of the discovery/use of biomimetic apatite-based colloidal nanoparticles in the field of nanomedicine, and in particular for diseased cells diagnosis thanks to improved cell uptake capabilities.…”

Section: +mentioning

confidence: 99%

Biomimetic Apatite-Based Functional Nanoparticles as Promising Newcomers in Nanomedicine: Overview of 10 Years of Initiatory Research

Drouet¹

2015

IMPH

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Biomimetic calcium phosphate apatites, analogous to bone mineral, may now be produced synthetically. Their intrinsic biocompatibility and the nanometer dimensions of their constitutive crystals not only allow one to envision applications in bone tissue regeneration, but also in other medical fields such as nanomedicine, and in particular in view of cell diagnosis.In this mini-review, we look back at 10 years of our dedicated research, and summarize the main advances made in terms of preparation, physical-chemical characterizations and biological evaluations of colloidal formulations of biomimetic apatite-based nanoparticles, which we illustrate here with the angle of cancer diagnosis. The confirmed exceptional biocompatibility of these engineered nanoparticles, associated to the possibility to confer them luminescence properties by way of controlled lanthanide doping, and their capacity to be internalized by cells, including with cancer cell addressing abilities (shown here as a proof of concept), underline that biomimetic apatite-based colloidal nanoparticles are particularly promising for nanomedicine applications, for example related to diseased cells diagnosis. Multidisciplinary research on these functional nanoparticles, initiated as described here, has now generated emulation in the scientific community where the concept of apatite nanoparticles for nanomedicine is being, gratifyingly, appropriated.

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“…A jelölésre alkalmazott, kristályrácsba beépített európium-foszfátot nanokristályos formában az irodalomban már használták sejtek nyomjelzésére, nem volt toxikus, így nem vártunk különösebb reakciót. [9][10][11] A primer kísérleti nyomószilárd-sága 118 MPa, porozitása nagyobb, mint a későbbiekben említendő GY3-é.…”

Section: Eredeti Közleményekunclassified

The examination of aerogel composite artificial bone substitutes in animal models

Szabó¹,

Kiss²,

Manó³

et al. 2013

Biomech Hung

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AbsztraktA Debreceni Egyetem Ortopédiai Klinikája, valamint a Szervetlen és Analitikai Kémiai Tanszéke egy OTKA pályázat keretében szuperkritikus körülmények között hidroxiapatitot, trikalcium-foszfátot és szilika aerogélt tartalmazó kompoz itokat állított elő, amely a csontban különböző okokból kialakult súlyos folytonossági hiány pótlására szolgálhat. Bár a kialakult csonthiány pótlására a saját csont beültetése lenne a legkedvezőbb, hiszen a szervezet azt fogadja be a legnagyobb valószínűséggel, azonban a donorterületek véges volumene miatt autograftok csak korlátozottan állnak a rendelkezésünkre. A kifejlesztett anyagunk viszont amellett, hogy oszteoinduktív és oszteokonduktív hatással is rendelkezik, olcsón és nagy mennyiségben előállítható. Az élő szervezetben való viselkedést felnőtt, hím, egészséges Long-Evans patkányok bal oldali combcsontján tanulmányoztuk. A létrehozott csontpótló anyagot a femur late ra lis condylusába készített 2 mm átmérőjű defektusba ültettük, majd pedig megfi gyeltük a beépü lését a 4., 8. és 13. héten röntgen-, illetve a 13. hét után szövettani vizsgálatok által. Az eddigi eredményeink alapján elmondható, hogy a szövettani metszeteken és a röntgenképeken csontújdonképződés mellett a vizsgálati anyag beépülése is megfi gyelhető volt, ugyanakkor csak rész-ben szívódott fel. A kutatás kimenetelét torzító, jelentős immunválaszt nem váltottak ki a vizsgálati anyagok. Mindezek alapján a munka folytatását tervezzük, megcélozva egy, a klinikai gyakorlatban bevezethető anyag előállítását.Kulcsszavak: csontpótló anyag, aerogél, szuperkritikus, Long-Evans, patkány, állatkísérleti mo dell, csont, femur, hidroxiapatit, trikálcium-foszfát, szilika aerogélThe examination of aerogel composite artifi cial bone substitutes in animal models Abstract At the University of Debrecen, Department of Orthopaedic Surgery and Department of Inorganic and Analytical Chemistry aimed to make a bone substitute which is cheap, easy to produce and it can be used in clinical practice. These supercritical circumstances made aerogel composites are from hidroxi-apatit, tricalcium-phosphat and silica alcogel. They also have osteoinductive, osteoconductive properties; furthermore they can provide a stable structure for the newly built bone. The research was carried out on adult, male, healthy Long-Evans rats. We implanted the

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Europium-doped bioapatite: a new photostable biological probe, internalizable by human cells

Cited by 150 publications

References 15 publications

Evaluation of short-term effects of rare earth and other elements used in magnesium alloys on primary cells and cell lines☆

Evaluation of short-term effects of rare earth and other elements used in magnesium alloys on primary cells and cell lines☆

Biomimetic Apatite-Based Functional Nanoparticles as Promising Newcomers in Nanomedicine: Overview of 10 Years of Initiatory Research

The examination of aerogel composite artificial bone substitutes in animal models

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