Evaluación de Diferentes Técnicas de Extracción del ADN de Hongos Filamentosos

Lurá, María C.; Benitez, Joel D.; Jáuregui, Soledad; González, A.M Castillo

doi:10.14409/fabicib.v7i1.716

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cassago et al, 2002;Lurá et al, 2003). The application of liquid nitrogen and the maceration along with the lysis buffer allowed a more efficient disruption, which solubilized a larger amount of nucleic acids, and ensured the deactivation of DNA degrading enzymes (Rønsted et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Molecular Identification of Trichoderma spp. in Garlic and Onion Fields and In Vitro Antagonism Trials on Sclerotium cepivorum

Alvarado-Marchena

Rivera-Méndez

2016

Rev. Bras. Ciênc. Solo

View full text Add to dashboard Cite

Trichoderma species are non-pathogenic microorganisms that protect against fungal diseases and contribute to increased crop yields. However, not all Trichoderma species have the same effects on crop or a pathogen, whereby the characterization and identification of strains at the species level is the first step in the use of a microorganism. The aim of this study was the identification -at species level -of five strains of Trichoderma isolated from soil samples obtained from garlic and onion fields located in Costa Rica, through the analysis of the ITS1, 5.8S, and ITS2 ribosomal RNA regions; as well as the determination of their individual antagonistic ability over S. cepivorum Berkeley. In order to distinguish the strains, the amplified products were analyzed using MEGA v6.0 software, calculating the genetic distances through the Tamura-Nei model and building the phylogenetic tree using the Maximum Likelihood method. We established that the evaluated strains belonged to the species T. harzianum and T. asperellum; however it was not possible to identify one of the analyzed strains based on the species criterion. To evaluate their antagonistic ability, the dual culture technique, Bell's scale, and the percentage inhibition of radial growth (PIRG) were used, evidencing that one of the T. asperellum isolates presented the best yields under standard, solid fermentation conditions.

show abstract

Section: Discussionmentioning

confidence: 99%