Evaluating bacterial community structures in oil collected from the sea surface and sediment in the northern Gulf of Mexico after the deepwater horizon oil spill

Abstract

On page 496, under the heading "Bacterial community in sediment and sediment overlying water", the terms SC and SG have been wrongly swapped in some places.The paragraph should read like the following:The pyrosequencing identified diverse bacterial groups in sediments from stations SG and SC, with 111 and 95 genera from 16 and 14 phyla, respectively (Table 1). In comparison, bacteria in the overlying water of SC sediment (SC-OW) were much less diverse, with 35 genera from 3125 OTUs. The bacterial communities in SG and SC sediments consisted mainly of proteobacteria, accounting for 64.1% and 69.3% of total bacteria, respectively (Table 1).Gamma-and Alphaproteobacteria together accounted for a major fraction of the proteobacteria in SG (42.5%) and SC (52.7%) sediments, whereas the Betaproteobacteria represented only 1.2-1.5% (Fig. 2). In the SC overlying water, proteobateria represented 87.6% of total OTUs (1601), of which Betaproteobacteria accounted for 51.2%, and Gamma-and Alphaproteobacteria together represented 34.4%. In addition, Bacteroidetes, Actinobacteria, and Planctomycetes occurred in all sediments and in overlying water. Cluster analysis showed that bacterial community structures of SC and SG sediments were similar, but those of the SC overlying water were different than the sediment communities (Fig. 3).The six places corrected are in bold type. The authors apologize for this and regret this oversight.

Examining the diversity of microbes in a deep-sea coral community impacted by the Deepwater Horizon oil spill

“…Overall, the dominance of Proteobacteria observed in this study is consistent with previous studies from DWH impacted environments including deep-sea sediments (Kimes et al, 2013, Liu and Liu 2013, Mason et al, 2014, the water-column (Hazen et al, 2010, Baelum et al, 2012, Mason et al, 2012, Redmond & Valentine 2012, Rivers et al, 2013, Yang et al, 2014 and coastal beach ecosystems (Koskta et al, 2011, Newton et al, 2013. These data are also consistent with the diverse number of already known oil degrading bacteria (for reviews see; Atlas, 1981, Head et al, 2007, Yakimov et al, 2007 (Rinke et al, 2013) that is based upon genomes sequences of several small cells including Candidatus Parvarchaeum, Candidatus Micrarchaeum, Candidatus Nanosalina, andCandidatus Nanosalinarum (Narasingaro et al, 2012, Baker et al, 2010).…”

“…These sediment communities resembled those previously observed in GoM sediments (e.g. Lanoil et al, 2001) suggesting that sediment microbial communities had returned to pre-spill community structures (Liu & Liu 2013).…”

“…1.5-3 cm in Kimes et al, 2013. One year after the spill, however, sediments examined close to the wellhead at 2km and 6km, were found to exhibit a diverse microbial community structure including Methylococcus, Methylobacter, Vibrio, Acidobacteria, Flavobacteria and Pseudomonas bacterial species (Liu & Liu 2013). These sediment communities resembled those previously observed in GoM sediments (e.g.…”

Effect of Pollution on Aquatic Microbial Diversity

“…The authors showed that members of Oceanospirillales can respond rapidly to aliphatic hydrocarbons as part of oil spill in marine environment. In another study, Liu and Liu (2013) investigated bacterial community structure in oil samples using next-generation sequencing of 16S rRNA including oil mousses collected on sea surface and salt marshes during the Deepwater Horizon oil spill and oil deposited in sediments adjacent to the wellhead 1 year after the spill. Based on molecular phylogeny of generated data, the authors found that Erythrobacter, Rhodovulum, Stappia, and Thalassospira belonging to Alphaproteobacteria were most prevalent in oil mousses, and the authors accounted their occurrence with high temperature and strong irradiance observed in surface water of the Gulf of Mexico.…”

Time Dependent Influence of Aerobic Heterotrophic Bacteria Cyanobacteria Interaction during Biodegradation of Poly Aromatic Hydrocarbons

“…Annweiller reported intermediates such as 2,3-dihydroxy naphthalene; 2-carboxynnarmic acid and phthalic and benzoic acid were identified in addition to typical metabolites of naphthalene degradation known from mesophiles in the pathway of B. thermoleovorans. Thalassospira is also implicated in degradation of fluorene and naphthalene and was found to be associated with oil on water surface during the DWH blowout previously by Liu and Liu [20,21].…”