Evaluating cell wall structure and composition of developing cotton fibers using Fourier transform infrared spectroscopy and thermogravimetric analysis

Abstract

Universal attenuated total reflectance Fourier transform infrared (UATR‐FTIR) spectroscopy and thermogravimetric analysis (TGA) were used to investigate the structural changes of cotton (Gossypium hirsutum L.) fibers as a function of developmental programming. The presence of noncellulosic compounds (wax, protein, hemicelluloses, pectic substances, amino acids, etc.) was evident from FTIR spectra of fibers at 10, 14, 17, and 20 dpa (day postanthesis). The vibration corresponding to the noncellulosic compounds disappeared at 36 dpa. Furthermore, independent TGA analysis supported the results obtained with FTIR, showing that the transition from primary cell wall synthesis to secondary cell wall synthesis occurs at or around 20 dpa. This study is the first to report on the use of the UATR‐FTIR and TGA to elucidate structural changes during cotton fiber development. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci, 2008

Fourier-Transform Imaging of Cotton and Botanical and Field Trash Mixtures

“…The presence of carbonyl compounds in a larger amount with the leaf compared to hull and stem was suggested. 1250 cm −1 [19]. In contrast, the hull, ( Figure 3C), and stem, (Figure 3D), had a doublet in the same fingerprint CH region (1500-1300 cm −1 ) with differences in intensities for these trash types, but hull had a C=O doublet at 1700 cm −1 [19], whereas the stem, ( Figure 3D) has a singlet peak in that region.…”

“…At 2900-2800 cm −1 the C-H stretch was present in both botanical trash spectral bands, as well as the cotton spectrum [15]. In the fingerprint region, including the CO and CH stretching region, between 1100-900 cm −1 , all spectra had the C-O bands and at 900 cm −1 a beta-linkage appears between two glucose units due to cellulose synthesis [19]. Cotton did not have a strong band at 1800-1500 cm −1 signifying the C=O stretch, whereas the trash spectra all have this fine structure [15].…”

“…Cotton did not have a strong band at 1800-1500 cm −1 signifying the C=O stretch, whereas the trash spectra all have this fine structure [15]. Among each other, the botanical trash types were distinguished by the fingerprint region having Figure 3B, had a distinctive CO band at 1750 cm −1 and a CH doublet between 1500-1250 cm −1 [19]. In contrast, the hull, (Figure 3C), and stem, (Figure 3D), had a doublet in the same fingerprint CH region (1500-1300 cm −1 ) with differences in intensities for these trash types, but hull had a C=O doublet at 1700 cm −1 [19], whereas the stem, ( Figure 3D) has a singlet peak in that region.…”

“…Among each other, the botanical trash types were distinguished by the fingerprint region having Figure 3B, had a distinctive CO band at 1750 cm −1 and a CH doublet between 1500-1250 cm −1 [19]. In contrast, the hull, (Figure 3C), and stem, (Figure 3D), had a doublet in the same fingerprint CH region (1500-1300 cm −1 ) with differences in intensities for these trash types, but hull had a C=O doublet at 1700 cm −1 [19], whereas the stem, ( Figure 3D) has a singlet peak in that region. The presence of carbonyl compounds in a larger amount with the leaf compared to hull and stem was suggested.…”

Efficacy of Aspergillus fumigatus R6 Pectinase in Enzymatic Retting of Kenaf

“…This indicates the hydrolysis of the pectin chain to form monomers. There was a decrease in absorbance at the peak around 2920 cm -1 , which was associated with the asymmetrical stretching vibration of CH2 in lignin and the presence of waxy substances (Abidi et al 2008). A new sharp peak found at 1730 cm -1 indicates the formation of aliphatic ketones.…”