Evaluating children in the Ukraine for colonization with the intestinal bacterium Oxalobacter formigenes, using a polymerase chain reaction-based detection system*

Sidhu, H.; Enatska, Lyubov; Ogden, Sharon; Williams, William N.; Allison, Milton J.; Peck, Ammon B.

doi:10.1016/s1084-8592(97)80015-x

Cited by 77 publications

(69 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…formigenes was detected in 77% of the present healthy volunteers, similar to the level of colonization reported for healthy adults in several studies [9,13,24], whereas only 37% of patients with urolithiasis were positive. These results suggest that O. formigenes may be associated with the formation of calcium oxalate stone.…”

Section: Discussionsupporting

confidence: 90%

“…Sidhu et al [15] suggested that the absence of O. formigenes from the intestinal tract of patients with cystic ®brosis appeared to lead to the increased absorption of oxalate, thereby increasing the risk of hyperoxaluria and its complications. The detection rate is 60±80% in a healthy population [9,13,24].…”

Section: Discussionmentioning

confidence: 99%

“…This gene has been cloned and sequenced [14]. Sequencing of the 5k ends of oxc from numerous isolates of O. formigenes identi®ed unique, highly conserved regions which permitted the synthesis of a genus-speci®c PCR primer pair [13,17]. This PCR primer pair ampli®es a 416 bp product (in group II strains) or a 413 bp product (in group I strains) of the 5k end of oxc that is unique to O. formigenes.…”

Section: Discussionmentioning

confidence: 99%

“…Kreader [26] used a procedure including proteinase K-hexadecyltrimethylammonium bromide-phenol extraction but the isolated DNA still contained PCR inhibitors. For further puri®cation of DNA, Stacy-Phipps et al [28] used glass matrix to adsorb DNA, which was used in the PCR analysis established by Sidhu et al [12,13] to detect O. formigenes in human feces. These DNA isolation and puri®cation procedures are time-consuming and therefore we used simpler sample preparation procedures.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, O. formigenes is reportedly suitable for DNA-based identi®cation [12,13]. O. formigenes expresses a unique gene, oxc, encoding oxalyl-CoA decarboxylase used in the catabolism of oxalic acid [14].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Molecular identification of Oxalobacter formigenes with the polymerase chain reaction in fresh or frozen fecal samples

Kwak¹,

Jeong²,

Lee

et al. 2001

BJU International

View full text Add to dashboard Cite

Objective To develop a simple and rapid polymerase chain reaction (PCR) method for detecting Oxalobacter formigenes (which degrades oxalate in the gut) in fecal specimens from healthy volunteers and patients with urolithiasis, and to determine whether O. formigenes can be detected in frozen or fresh fecal samples. Materials and methods Whole bacterial DNA was isolated directly from fresh and frozen fecal samples obtained from 30 healthy volunteers free from urolithiasis and from fresh fecal samples obtained from 38 patients with urolithiasis. Genus-speci®c oligonucleotide sequences were designed, corresponding to homologous regions residing in the oxc gene that encodes for oxalyl-coenzyme A decarboxylase. A PCR-based assay was used on both fresh and frozen fecal samples, and the nucleotide sequences analysed to con®rm oxc. Results A PCR product of 416 bp encoding the oxc gene was detected in 23 (77%) of 30 healthy volunteers free from urolithiasis and in 14 (37%) of 38 patients with urolithiasis. In healthy volunteers, the results of PCR for the fresh and the frozen samples were identical in each subject. The nucleotide sequence analysis showed that the sequence of the ampli®ed product was compatible with that of oxc. Conclusion O. formigenes can be identi®ed easily and ef®ciently using this PCR-based detection system. The colonization rate of O. formigenes in patients with urolithiasis was signi®cantly lower than that in healthy volunteers known to be free from urolithiasis. Furthermore, as the PCR-based assay results in the frozen fecal samples were identical to those from fresh samples in each subject, immediate processing of fecal samples may not be necessary to detect O. formigenes in the clinical setting.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular identification of Oxalobacter formigenes with the polymerase chain reaction in fresh or frozen fecal samples

Kwak¹,

Jeong²,

Lee

et al. 2001

BJU International

View full text Add to dashboard Cite

show abstract

Oxalobacter

Allison¹,

MacGregor²,

Stahl³

2015

Bergey's Manual of Systematics of Archaea and Bacteria

View full text Add to dashboard Cite

Ox.al.o.bac' ter . Gr. n. oxal pertaining to oxalate; M.L. n. bacter the masculine form of the Gr. neut. n. bactrum a rod; M.L. masc. n. Oxalobacter an oxalate rod. Proteobacteria / Betaproteobacteria / Burkholderiales / Oxalobacteraceae / Oxalobacter Straight or curved to vibrioid , Gram‐negative , nonsporeforming rods 0 . 4–0 . 6 × 1 . 0–2 . 5 μ m in length . Flagella may be present or absent. Strictly anaerobic . Chemoorganotroph. Oxalate is used as the major carbon and energy source . Oxamate may also be used , but neither carbohydrates nor any of a wide variety of other compounds will replace oxalate as the growth substrate . Acetate is assimilated for cell synthesis and is required by some, and perhaps by all, strains. Oxalate utilization is accompanied by alkalization of the medium, and formate is produced in approximately equimolar proportions to the amount of oxalate metabolized. Strains have been isolated from the rumens of cattle and sheep, from cecal and fecal samples from humans, guinea pigs, swine, domestic and wild rats, and from freshwater lake and marine sediments. It is probable that these bacteria colonize many other anaerobic habitats. Oxalobacter is currently classified in the class Betaproteobacteria , the order Burkholderiales , and the family Oxalobacteraceae . The mol % G + C of the DNA is : 48–52. Type species : Oxalobacter formigenes Allison, Dawson, Mayberry and Foss 1985b, 375 (Effective publication: Allison, Dawson, Mayberry and Foss 1985a, 6.)

show abstract