<i>Oxalobacter</i>

Allison, Milton J.; MacGregor, Barbara J.; Stahl, David A.

doi:10.1002/9781118960608.gbm00966

Cited by 2 publications

(1 citation statement)

References 21 publications

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“…The reference genome database includes organisms available in GenBank as of November 2009 and can be accessed at http://www.hmpdacc.org/HMREFG/. We used this dataset to examine prevalence of O. formigenes and to distinguish between O. formingenes Group I and Group II which are represented by strains OXCC13 and strain HOxBLS, respectively 22 . In the second approach, metagenomic data were taxonomically profiled using MetaPhlAn 21 , permitting estimation of relative abundances for the different taxa, with the output of the analysis at www.hmpdacc.org/HMSMCP .…”

Section: Methodsmentioning

confidence: 99%

The Presence of Oxalobacter formigenes in the Microbiome of Healthy Young Adults

et al. 2016

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Oxalobacter formigenes, a member of the human colonic microbiota that plays a major role in net colonic oxalate transport and secretion, is protective against formation of calcium oxalate kidney stones. We now describe the prevalence, relative abundance and stability of O. formigenes in healthy young adults in the United States, as revealed by Human Microbiome Project (HMP) data from fecal samples from 242 healthy young adults who had 1-3 study visits. Samples underwent whole-genomic shotgun (WGS) sequencing, and/or 16S rRNA sequencing. Three datasets available from the processed sequence data were studied: WGS metagenomic analysis by alignment to reference genomes (HMSCP) or using MetaPhlAn, or QIIME analysis of the V1-3 or V3-5 16S sequences. O. formigenes was detected in fecal samples using both the WGS and 16S rRNA data. Analysis of the WGS dataset, using HMSCP, showed that 29 (31%) of 94 subjects were O. formigenes-positive while the V1-3 and V3-5 analyses were less sensitive for O. formigenes detection. When present, O. formigenes relative abundance varied over 3 log10, and was normally distributed. For 66 samples studied by all three methods, all assays agreed in 58 (88%). Of 14 subjects who were O. formigenes-positive at baseline, 13 (93%) were positive on follow-up visit, indicating the stability of colonization. O. formigenes appears to be stably present in fewer than half of healthy young USA adults and is most sensitively detected by WGS.

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Section: Methodsmentioning

confidence: 99%

The Presence of Oxalobacter formigenes in the Microbiome of Healthy Young Adults

et al. 2016

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Forty Years of Oxalobacter formigenes, a Gutsy Oxalate-Degrading Specialist

Daniel

Moradi

Paiste

et al. 2021

Appl Environ Microbiol

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Oxalobacter formigenes , a unique anaerobic bacterium that relies solely on oxalate for growth, is a key oxalate-degrading bacterium in the mammalian intestinal tract. Degradation of oxalate in the gut by O. formigenes plays a critical role in preventing renal toxicity in animals that feed on oxalate-rich plants. The role of O. formigenes in reducing the risk of calcium oxalate kidney stone disease and oxalate nephropathy in humans is less clear, in part due to difficulties in culturing this organism, and the lack of studies which have utilized diets controlled in their content of oxalate. Herein, we review the literature on the 40 th anniversary of the discovery of O. formigenes , with a focus on its biology, its role in gut oxalate metabolism and calcium oxalate kidney stone disease, and potential areas of future research. Results from ongoing clinical trials utilizing O. formigenes in healthy volunteers and in patients with Primary Hyperoxaluria Type 1 (PH1), a rare but severe form of calcium oxalate kidney stone disease, will also be discussed. Information has been consolidated on O. formigenes strains and best practices to culture this bacterium, which should serve as a good resource for researchers.

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Oxalobacter

Cited by 2 publications

References 21 publications

The Presence of Oxalobacter formigenes in the Microbiome of Healthy Young Adults

The Presence of Oxalobacter formigenes in the Microbiome of Healthy Young Adults

Forty Years of Oxalobacter formigenes, a Gutsy Oxalate-Degrading Specialist

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