Evaluating Effects of AIV Infection Status on Ducks Using a Flow Cytometry-Based Differential Blood Count

Jax, Elinor; Werner, E G Mueller; Müller, Inge; Schaerer, Beatrice; Kohn, Marina; Olofsson, Jenny; Waldenström, Jonas; Kraus, Robert H. S.; Härtle, Sonja

doi:10.1128/spectrum.04351-22

Cited by 3 publications

(2 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been well known that a small population of chicken splenic macrophages stain for chicken B-cell marker chB6 ( Figure 4C ) ( 47 ). Therefore, the BAFF-R mAb is a more specific reagent for chicken B cells ( 73 ). Of note, MRC1L-B may be sensitive to enzymatic digestion.…”

Section: Mononuclear Phagocytic Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Delineation of chicken immune markers in the era of omics and multicolor flow cytometry

Härtle,

Sutton,

Vervelde

et al. 2024

Front. Vet. Sci.

Self Cite

View full text Add to dashboard Cite

Multiparameter flow cytometry is a routine method in immunological studies incorporated in biomedical, veterinary, agricultural, and wildlife research and routinely used in veterinary clinical laboratories. Its use in the diagnostics of poultry diseases is still limited, but due to the continuous expansion of reagents and cost reductions, this may change in the near future. Although the structure and function of the avian immune system show commonalities with mammals, at the molecular level, there is often low homology across species. The cross-reactivity of mammalian immunological reagents is therefore low, but nevertheless, the list of reagents to study chicken immune cells is increasing. Recent improvement in multicolor antibody panels for chicken cells has resulted in more detailed analysis by flow cytometry and has allowed the discovery of novel leukocyte cell subpopulations. In this article, we present an overview of the reagents and guidance needed to perform multicolor flow cytometry using chicken samples and common pitfalls to avoid.

show abstract

Section: Mononuclear Phagocytic Cellsmentioning

confidence: 99%

“…Shortly after chB6 expression becomes detectable, pre-bursal B cells begin to express another pan-B-cell marker, the BAFF receptor (BAFF-R), recognized by mAb anti-BAFF-R clone 2C4 ( 73 ) and at ED14, chB6+ cells in the spleen are all BAFF-R+ ( 82 ).…”

Section: B Cellsmentioning

confidence: 99%

Delineation of chicken immune markers in the era of omics and multicolor flow cytometry

Härtle,

Sutton,

Vervelde

et al. 2024

Front. Vet. Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

The contribution of individual characteristics of <i>Anas</i> and <i>Aythya</i> individuals to their susceptibility to low‐pathogenic avian influenza viruses in the south of Western Siberia

Druzyaka,

Sobolev

et al. 2024

Ûg Ross.: èkol. razvit.

View full text Add to dashboard Cite

Aim. Analysis of the influence of species, sex, and age of genus Anas and Aythya individuals on their sensitivity to low pathogenic avian influenza viruses (LPAIV) in the south of Western Siberia. Material and Methods. Infestation of ducks with the influenza virus was determined by cloacal swabs obtained from 5014 individuals of ducks belonging to the genus Anas and Aythya during the autumn migration period in the south of Western Siberia. Isolation of influenza A virus isolates was carried out according to standard WHO methods. The distribution of infected individuals was estimated with ꭓ2. Relative risk (RR) was used to estimate the risk of infection for each individual species. Results. Among the examined ducks, the distribution between species differed between infected and uninfected individuals. The common teal was under greater risk of being infected with the avian influenza virus. In this species, the sex of the individual influenced the probability of infestation both in young individuals and the whole age spectrum. Conclusions. Individual characteristics of wild dabbling and diving ducks (species, sex, age) affect their infestation with low‐pathogenic avian influenza virus during the autumn migration period in the south of Western Siberia.

show abstract

Characterization of class-switched B cells in chickens

von La Roche,

Schumacher,

Kohn

et al. 2024

Front. Immunol.

View full text Add to dashboard Cite

While B cell development in the birds’ primary B cell organ, the bursa Fabricius, is relatively well understood, very little is known about post bursal B cell differentiation into plasma and memory cells though these cells are essential for a protecting antibody response and so far, no specific markers for these cells were available. Since immunoglobulin class switch is one part of the B cell differentiation process, our objective was to conduct a first detailed investigation of class-switched chicken B cells. As only very few IgY and IgA expressing cells were detected in lymphoid organs of young chickens, we used CD40L and IL-10 to establish a prolonged in vitro culture system, which induces B cell proliferation, class switch to IgY and IgA and enhanced antibody secretion. This enabled a phenotypic analysis of differentiating B cells. Importantly, these cells lost surface expression of the B cell markers chB6 and BAFF-R. B cell receptor surface expression remained unchanged, showing that while differentiating toward plasma cells, B cells can be addressed by L chain staining. Newly generated potential plasma cell markers CD138 and TACI showed only a transient expression on cultured cells and rather act as markers for B cell activation than plasma/memory cells in general. CD57 upregulation was connected to activation and blast formation but not to class switch. We also examined potential changes in class-switched cells in different age groups and post vaccination. Surprisingly, bursa involution, laying and age had no distinct effects on the presence of class-switched cells, but we detected significantly more class-switched B cells post vaccination. Hence, we are now able to generate class-switched plasmablasts in vitro for a more detailed characterization and can address them under different conditions in chickens for further analysis of their B cell response.

show abstract

Evaluating Effects of AIV Infection Status on Ducks Using a Flow Cytometry-Based Differential Blood Count

Abstract: Outbreaks of avian influenza in poultry and humans are a global public health concern. Aquatic birds are the primary natural reservoir of avian influenza viruses (AIVs), and strikingly, AIVs mainly cause asymptomatic or mild infection in these species.

Cited by 3 publications

References 42 publications

Delineation of chicken immune markers in the era of omics and multicolor flow cytometry

Delineation of chicken immune markers in the era of omics and multicolor flow cytometry

The contribution of individual characteristics of <i>Anas</i> and <i>Aythya</i> individuals to their susceptibility to low‐pathogenic avian influenza viruses in the south of Western Siberia

Characterization of class-switched B cells in chickens

Contact Info

Product

Resources

About