Evaluating potential risks of food allergy of novel food sources based on comparison of proteins predicted from genomes and compared to www.AllergenOnline.org

Abdelmoteleb, Mohamed; Zhang, Chi; Furey, Brian; Kozubal, Mark A.; Griffiths, Hywel; Champeaud, Marion; Goodman, Richard E.

doi:10.1016/j.fct.2020.111888

Cited by 52 publications

(31 citation statements)

References 54 publications

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“…Moreover, enolase (G3GDU2_PENVA), with 117 amino acid residues, has the same sequence as residues 178–294 of phosphopyruvate hydratase (O96656_PENVA). Homologous amino acid sequences are one of the important factors in predicting allergic activity (Abdelmoteleb et al., 2021). Therefore, phosphopyruvate hydratase was predicted to be a potential allergen, and it may have the same domain for allergic reactions with enolase.…”

Section: Resultsmentioning

confidence: 99%

Characterization and epitope prediction of phosphopyruvate hydratase from Penaeus monodon (black tiger shrimp)

Yang

Liu

Zeng

et al. 2021

Journal of Food Science

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Shellfish allergies constitute an important cause of food-induced anaphylactic reactions, which pose challenges to food safety and human health worldwide. In the present study, the specific IgE (sIgE) binding characteristics of different shrimp proteins of black tiger shrimp (Penaeus monodon) to the sera of eight shrimp-allergic patients from China were studied by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and nanoliquid chromatography time-of-flight mass spectrometry. According to the PLGS scores (>2000) and the sequence coverage (>40%), eight proteins with sIgE binding activity were identified, including myosin heavy chain type 1 (K4Q4N8), hemocyanin (G1AP69 and Q95V28), phosphopyruvate hydratase (O96656), arginine kinase (C7E3T4), tropomyosin (A1KYZ2), sarcoplasmic calcium binding protein (H7CHW2) and glyceraldehyde-3-phosphate dehydrogenase (A0A097BQP2). Among these eight proteins, phosphopyruvate hydratase was a prevalent IgEbinding protein among these Chinese patients with binding observed in 100% of sera. Moreover, 13 peptides were predicted as epitopes of phosphopyruvate hydratase. These new details help us to understand the crustacean IgE-binding proteins especially Penaeus monodon IgE-binding proteins, that would cause allergic reaction to Chinese patients. And our findings may provide essential information to improve allergy prevention and clinical treatment to shrimp allergy in China.Practical Application: This research may have diagnostic and therapeutic value for shrimp allergies in China. K E Y W O R D Sallergen, epitope, Penaeus monodon, phosphopyruvate hydratase INTRODUCTIONFood allergies are a pathological disorder of the immune system with adverse reactions, including skin irritation, diarrhea, respiratory disease and even syncope, and affect approximately 1%−5% of adults and at least 4%−8% of children worldwide (Monaci et al., 2018;Wang et al., 2009). In recent years, the prevalence of food allergies has increased and thus has aroused new public concern for food safety.Although hundreds of foods are known to cause food allergies, eight food groups, including eggs, fish, milk, peanuts, crustacean shellfish, soy, tree nuts, and wheat, are considered priority allergens and account for over 90%

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Section: Resultsmentioning

confidence: 99%

Characterization and epitope prediction of phosphopyruvate hydratase from Penaeus monodon (black tiger shrimp)

Yang

Liu

Zeng

et al. 2021

Journal of Food Science

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show abstract

“…The highly conservative current approach appears to lead to a high number of false positives (Ladics et al, 2007 ; Abdelmoteleb et al, 2021 ; Herman et al, 2021 ). A full FASTA approach with appropriate match criteria has claimed to be as sensitive as the 35% identity over an 80‐aa sliding window approach, while the specificity is significantly higher (Ladics et al, 2007 ; Silvanovich et al, 2009 ; Abdelmoteleb et al, 2021 ). Conversely, there are studies reporting experimental IgE cross‐reactivity between proteins despite a low sequence identity (i.e.…”

Section: Assessmentmentioning

confidence: 99%

“…These also include alternative or complementary approaches beyond sequence alignment principles as defined by Codex. Some selected examples of alternative in silico approaches are (i) increasing the match criteria above 35% identity and decreasing the E ‐score below 1e ‐7 or smaller (Abdelmoteleb et al, 2021 ); (ii) numerical descriptors representing the physicochemical properties of the amino acid in protein sequence and machine learning approach for classification of allergens (Dimitrov et al, 2013 , 2014a ); (iii) similarity of their 3D protein structure as well as their amino acid sequence (Maurer‐Stroh et al, 2019 ); (iv) similarity search to a data set of allergenic and non‐allergenic proteins represented as binary fingerprints (Dimitrov et al, 2014b ); (v) machine learning approaches based on mapping of IgE epitope, motif search and/or other selected variables (Westerhout et al, 2019 ; Sharma et al, 2021 ); (vi) as well as novel approaches considering human leucocyte antigens (HLA) binders from known allergens for the in silico assessment of the sensitisation potential of innovative/novel proteins (Dimitrov & Atanasova, 2020 ).…”

Section: Assessmentmentioning

confidence: 99%

Scientific Opinion on development needs for the allergenicity and protein safety assessment of food and feed products derived from biotechnology

Mullins¹,

Bresson²,

Dalmay³

et al. 2022

EFS2

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This Scientific Opinion addresses the formulation of specific development needs, including research requirements for allergenicity assessment and protein safety, in general, which is urgently needed in a world that demands more sustainable food systems. Current allergenicity risk assessment strategies are based on the principles and guidelines of the Codex Alimentarius for the safety assessment of foods derived from 'modern' biotechnology initially published in 2003. The core approach for the safety assessment is based on a 'weight-of-evidence' approach because no single piece of information or experimental method provides sufficient evidence to predict allergenicity. Although the Codex Alimentarius and EFSA guidance documents successfully addressed allergenicity assessments of single/ stacked event GM applications, experience gained and new developments in the field call for a modernisation of some key elements of the risk assessment. These should include the consideration of clinical relevance, route of exposure and potential threshold values of food allergens, the update of in silico tools used with more targeted databases and better integration and standardisation of test materials and in vitro/in vivo protocols. Furthermore, more complex future products will likely challenge the overall practical implementation of current guidelines, which were mainly targeted to assess a few newly expressed proteins. Therefore, it is timely to review and clarify the main purpose of the allergenicity risk assessment and the vital role it plays in protecting consumers' health. A roadmap to (re)define the allergenicity safety objectives and risk assessment needs will be required to inform a series of key questions for risk assessors and risk managers such as 'what is the purpose of the allergenicity risk assessment?' or 'what level of confidence is necessary for the predictions?'.

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“…Novel food and novel food sources have encouraged interest of the scientific community, producers and consumers to the development of new and innovative products and technologies, as well as research into possible health benefits and / or risks (Knežević et. al, 2012;Caporgno and Mathys, 2018;Majid et al 2018;Mazzucchelli et al, 2018;Cappelli et al, 2020;Abdelmoteleb et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Novel Food legislation and consumer acceptance - importance for the food industry

Knežević

Grbavac²,

Palfi³

et al. 2021

Emir J Food Agric

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Novel foods are defined as food and food ingredients that have not been used to any significant extent in a particular country. This paper offers a brief overview of the current novel food legislation in European Union, Great Britain, USA, Australia and New Zealand, Canada and China. Prior to sale, food business operators (manufacturers or importers) are required, under different regulations and procedures, to submit information to Food Safety Authority about the product in question for a safety assessment. The approaches and specific information used to assess the safety of novel foods are outlined in national Guidelines. Generally, applicant should provide a detailed description of the novel food (identity of the novel food, production process, compositional data, proposed uses and use levels and anticipated intake of the novel food, history of use of the novel food and/or of its source, absorption, distribution, metabolism and excretion, nutritional information, toxicological information and allergenicity) for the safety assessment and market approval of a novel food.

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Evaluating potential risks of food allergy of novel food sources based on comparison of proteins predicted from genomes and compared to www.AllergenOnline.org

Cited by 52 publications

References 54 publications

Characterization and epitope prediction of phosphopyruvate hydratase from Penaeus monodon (black tiger shrimp)

Characterization and epitope prediction of phosphopyruvate hydratase from Penaeus monodon (black tiger shrimp)

Scientific Opinion on development needs for the allergenicity and protein safety assessment of food and feed products derived from biotechnology

Novel Food legislation and consumer acceptance - importance for the food industry

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