Evaluating the Effectiveness of Screened Lactic Acid Bacteria in Improving Crop Residues Silage: Fermentation Parameter, Nitrogen Fraction, and Bacterial Community

He, Liwen; Wang, Yimin; Guo, Xiang; Chen, Xiaoyang; Zhang, Qing

doi:10.3389/fmicb.2022.680988

Cited by 4 publications

(1 citation statement)

References 33 publications

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“…The use of L. plantarum starters during silage production ensures sufficient lactic acid production in the early stages of fermentation, which rapidly lowers the pH and inhibits the growth of putrefactive microorganisms [31,32]. Our study demonstrated an increase in lactate and acetate production following inoculation, resulting in a lower pH similar to that reported in literature [33].…”

Section: Discussionsupporting

confidence: 81%

The Effect of Lactobacillus planturum YQM48 Inoculation on the Quality and Microbial Community Structure of Alfalfa Silage Cultured in Saline-Alkali Soil

et al. 2023

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Alfalfa cultivated in salt–alkali soil was used for fermentation, to which an inoculum of Lactobacillus plantarum YQM48 was added, to assess its effect on the feed quality and the microbial community structure of the fermented silage. A control was included without inoculum. The nutritional components, fermentation quality, pH, and microbial community of the silage were measured after 30 and 60 days of anaerobic fermentation. The results showed that after 30 and 60 days of fermentation in the presence of the inoculum, the content of water-soluble carbohydrates, crude protein content, and dry matter were all significantly higher than those of the control silage, the pH and butyric acid content were lower, and the content of lactic acid and acetic acid were higher (p < 0.05 for all). There was no significant difference in pH and butyric acid content between 30 and 60 days of fermentation in the presence of the inoculum (p > 0.05), while the lactic acid and acetic acid contents were significantly lower in the 60-day silage compared to the 30-day silage (p < 0.05). Fermentation reduced the abundance of Cyanobacteria and Proteobacteria (the abundant phyla in the fresh alfalfa), while the abundance of Firmicutes increased, reaching 92.3% after 60 days of fermentation with inoculum. The dominant genus in that sample was Lactobacillus (70.0%), followed by Enterococcus (12.9%), while fermentation of 60 days without inoculum resulted in only 29.2% Lactobacillus, together with 27.8% Cyanobacteria, and 12.2% Enterococcus. In summary, the addition of L. plantarum YQM48 can improve the nutritional components and fermentation quality of alfalfa silage cultivated on a salt-alkali soil.

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Section: Discussionsupporting

confidence: 81%

The Effect of Lactobacillus planturum YQM48 Inoculation on the Quality and Microbial Community Structure of Alfalfa Silage Cultured in Saline-Alkali Soil

et al. 2023

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Fermentation quality and bacterial community of delayed filling stylo silage in response to inoculating lactic acid bacteria strains and inoculating time

Tian

Huang

Tian

et al. 2023

Chem. Biol. Technol. Agric.

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Background Filling silos generally takes much time in practice, which may negatively affect silage fermentation and feed intake. To clarify the effects of inoculating time, lactic acid bacteria (LAB) strains and filling time on the silage fermentation and microbes of stylo (Stylosanthes guianensis) and its silage, ensiling was quickly performed (quickly filling, QF) with or without LAB (Lactobacillus plantarum SXC48, Lb. plantarum CCZZ1 and Enterococcus faecalis XC124), and was performed after stylo inoculated with or without LAB was placed for 1 day (delayed filling, DF1) and 2 days (DF2). Results Delayed filling significantly increased pH, buffering capacity, microbial counts and lactic acid, acetic acid and NH3–N contents of stylo prior to ensiling. Inoculating Lb. plantarum SXC48 and CCZZ1 improved the fermentation quality of QF silage, indicated by more lactic acid, as well as lower pH and butyric acid content. Inoculating time significantly affected the pH and lactic acid content of silages. For the DF2 silages, inoculating SXC48 at filling was better than at chopping, while inoculating CCZZ1 had good fermentation quality, regardless of inoculating time. The results of 16S rRNA sequencing indicated that delayed filling enhanced the bacterial diversity of materials and silage, and inoculating significantly changed the composition of silage microbes. Kosakonia, Pseudomonas and Pantoea jointly dominated the fresh material, while Pantoea and Lelliottia jointly dominated the DF2 material. For the QF silages, inoculating SXC48 and CCZZ1 increased the relative abundance of Lactobacillus from 16.4% in the control silage to 76.5% and 82.0%, respectively. Pantoea and Lelliottia jointly dominated the DF silages. Inoculating SXC48 and CCZZ1 also increased the relative abundance of Lactobacillus in the DF stylo silages. Conclusions Under the present research conditions, delayed filling increased the lactic acid content and reduced the acetic acid, propionic acid and NH3–N contents of stylo silage, however, increased the bacterial diversity and relative abundance of undesirable bacteria, such as Pantoea and Lelliottia. The inoculating effectiveness varied with the LAB strains and inoculating time. Inoculating strian SXC48 at filling was better than at chopping, while inoculating strian CCZZ1 at both chopping and filling obtained the similar benefit. Graphical Abstract

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Effects of growth stage on the fermentation quality, microbial community, and metabolomic properties of Italian ryegrass (Lolium multiflorum Lam.) silage

et al. 2023

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IntroductionThis study aimed to investigate the effects of different growth stages (booting period-SYK; initial flowering-SCK; full flowering-SSK) on the fermentation quality, microbial community, metabolic pathways and metabolomic characteristics of Italian ryegrass silage.MethodsSingle molecule real-time (SMRT) sequencing and ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS/MS) were used to analyze bacterial communities and metabolites, respectively.ResultsAfter 60 d of fermentation, SYK had the lowest pH and the highest lactic acid content, which were significantly different from the other groups. The bacteria with the highest abundance in SYK, SCK and SSK groups were Lactiplantibacillus plantarum (63.98%), Weissella minor (28.82%) and Levilactobacillus brevis (64.81%), respectively. In addition, among the main differential metabolites in different growth stages, the number of amino acids was the most, and the corresponding metabolic pathways were mainly amino acid metabolic pathways. The biosynthesis of phenylalanine, tyrosine and tryptophan was significantly enriched (p<0.01) at booting stage and full flowering stage. Purine metabolism and ABC transporter pathway were significantly enriched at the initial flowering (p<0.001). Lactiplantibacillus plantarum had a negative correlation with xanthine and ganoderic acid F. Weissella minor had a positive correlation with D-Mannose and ganoderic acid F. Levilactobacillus brevis had a positive correlation with xanthine, and Latilactobacillus sakei had a positive correlation with cinnamic acid, D-Mannose, 2-Hydroxycinnamic acid and uridine.DiscussionIn conclusion, this study reveals the interaction mechanisms between ryegrass raw materials at different growth stages and epiphytic microorganisms during ensiling fermentation, providing new ideas for screening functional lactic acid bacteria, and laying a theoretical foundation for the production of safe and high-quality silage.

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Evaluating the Effectiveness of Screened Lactic Acid Bacteria in Improving Crop Residues Silage: Fermentation Parameter, Nitrogen Fraction, and Bacterial Community

Cited by 4 publications

References 33 publications

The Effect of Lactobacillus planturum YQM48 Inoculation on the Quality and Microbial Community Structure of Alfalfa Silage Cultured in Saline-Alkali Soil

The Effect of Lactobacillus planturum YQM48 Inoculation on the Quality and Microbial Community Structure of Alfalfa Silage Cultured in Saline-Alkali Soil

Fermentation quality and bacterial community of delayed filling stylo silage in response to inoculating lactic acid bacteria strains and inoculating time

Effects of growth stage on the fermentation quality, microbial community, and metabolomic properties of Italian ryegrass (Lolium multiflorum Lam.) silage

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