Evaluating the potential of an amelogenin-derived peptide in tertiary dentin formation

Peng, Xiaoyan; Han, Sili; Wang, Kun; Ding, Lei; Liu, Zhenqi; Zhang, Linglin

doi:10.1093/rb/rbab004

Cited by 5 publications

(4 citation statements)

References 35 publications

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“…(QPX)5 was the major adsorption region with hydroxyapatite (HAP), both (QPX)5 and C-tail inhibited nucleation, and C-tail contributed more to improve the HAP orientation degree (Wang, Hu, et al 2020). We further verified that the peptide could also induce the formation of tertiary dentin (Peng et al 2021). Therefore, this biomimetic peptide has the potential to exert a dual effect for deep caries repair.…”

Section: Introductionmentioning

confidence: 67%

Odontogenesis by Endocytosis of Peptide Embedding Bioactive Glass Composite

Liu

Wang

et al. 2022

J Dent Res

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Limited therapeutic options are available for treating deep caries. Those materials with potential of a dual effect to remineralize hard tissue and regenerate defective dentin tissues could be used as a new strategy for deep caries treatment. However, the application of the single component remains a challenge mainly because they lack calcium and phosphorus, are easily degraded, and are difficult to retain in the intricate body fluid environment. Considering the abundant source of calcium and phosphorus as well as the delivery performance of mesoporous bioactive glass (MBG), an amelogenin-derived peptide (QP5), which has a significant role in hard tissue remineralization, was loaded to fabricate a novel composite. After the synthesis of highly ordered MBG using a sol-gel method, the QP5 peptide was loaded increasingly by its extensive porous structure and enhanced electrostatic absorption. When used in an acidic environment, the MBG/QP5 composite presented pH-responsiveness, releasing therapeutic ions and functional peptides in a sequential cascade, and eventually adjusted the pH to a neutral state. The composite was internalized by dental pulp cells through a clathrin-mediated pathway and influenced by cell membrane lipid raft regulation. It could be also transported through the macro-pinocytotic pathway. Compared to the single treatment of peptide QP5 in 48 h, the composite facilitated a higher level of retention of the intracellular peptides. The composite further promoted migration and odontogenesis of dental pulp cells, including the improved activity of alkaline phosphatase, increased formation of mineralized nodules, and upregulated expression of mineralization-related genes compared to using MBG or QP5 alone. The composite further induced the dentin-like layer in a rat pulp capping model. The results suggested that this intelligent material with pH-responsiveness provides a promising alternative treatment method for biomimetic restoration of deep caries.

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Section: Introductionmentioning

confidence: 67%

Odontogenesis by Endocytosis of Peptide Embedding Bioactive Glass Composite

Liu

Wang

et al. 2022

J Dent Res

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“…Our study presents innovative findings on our previously developed amelogenin-derived peptide, TVH-19, which has been demonstrated as a multifunctional peptide for antibacterial, enamel remineralization, and inflammation prevention in dental pulp. − However, the precise mechanisms underlying these effects remain elusive. In this study, we first investigated the underlying mechanism of TVH-19 multifaced effects on DPCX based on its structure–activity relationship.…”

Section: Introductionmentioning

confidence: 99%

Amelogenin-Inspired Autoadaptive Peptide in a Caries Microenvironment Facilitates Long-Term Protection of the Dentin–Pulp Complex

Han,

Wang,

Peng

et al. 2024

ACS Appl. Mater. Interfaces

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Dental caries, one of the most prevalent infectious diseases, is the primary contributor to the early loss of natural teeth and is a significant public health issue. Known as the tooth's bioactive core, the dentin−pulp complex (DPCX) comprises tightly connected hard and soft tissues that not only serve as a biological barrier for the inner tooth tissue but also produce reparative dentin following mild disruptions. While efforts to preserve DPCX are numerous, most strategies focus on temporary antibacterial measures, inflammation reduction, or tissue regeneration, lacking a comprehensive, long-lasting solution. In this study, TVH-19, an autoadaptive peptide mimicking the pH-and ion-responsive capacity of amelogenin, was designed to exert multifaceted preservation of DPCX, providing a comprehensive strategy for preserving vital pulp. Leveraging its unique amphiphilicity-related cell penetration and ion/pH-responsive self-assembly properties, TVH-19 outperforms conventional pulp preservation materials by being capable of rapid cell penetration, minimizing diffused side effects, providing environment-responsive self-assembly/disassembly for balanced long-term antibacterial and cell protection, and facilitating the formation of lysosomal-escaping intracellular aggregates for the continuous activation of PDGFRα + dental pulp stem cells.

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“…To avoid those shortcomings, peptides are gaining increasing interests over proteins, thanks to their advantages such as ease of synthesis and resistance to enzymatic digestion. Importanly, previous work has already reported the potentiality of a peptide derived from amelogenin in driving dentine regeneration (Peng et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

LAMA5‐inspired adhesive dodecapeptide facilitates efficient dentine regeneration: An in vitro and in vivo study

Tang,

Wang,

et al. 2023

Int Endodontic J

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AimThe primary goal of this study was to investigate the potential effects of A5G81 in inducing reparative dentine (RD) formation both in vitro and in vivo.MethodologyCell adhesion was observed by crystal violet staining and quantified by Sodium Dodecyl Sulphate (SDS) extraction. Cell proliferation was investigated using Cell Counting Kit‐8 (CCK‐8) assay. Spreading of cytoskeleton was visualized using immunofluorescence staining. Protein expression level of Akt signalling pathway was compared in a human Akt pathway phosphorylation array. Genes that were up or downregulated by A5G81 were identified by RNA sequencing. The mRNA expression of odontoblastic markers was detected by quantitative real‐time polymerase chain reaction (qPCR). Moreover, mineralization of human dental pulp cells (hDPCs) was visualized by alizarin red staining and quantified using cetylpyridinium chloride (CPC). A direct pulp‐capping model was established in SD rats and the RD formation at 2 weeks after operation was observed using HE staining.ResultsA5G81 (optimal coating concentration: 0.5 mg/mL) promoted hDPCs adhesion and proliferation to a level that was similar to Type I collagen (COL‐1). Meanwhile, A5G81 activated Akt signalling pathway, albeit to a lesser extent than COL‐1. An inhibition test indicated that A5G81 induced hDPCs adhesion by activating PI3K pathway. A5G81 induced the expression of ECM remodelling genes and odontoblastic genes, which were demonstrated by RNA‐seq and qPCR, respectively. In addition, A5G81 efficiently accelerated the mineralization of hDPCs in both immobilized and soluble forms, a property that makes it more applicable in dental clinic. Finally, the pulp‐capping study in rats suggested that use of A5G81 could successfully induce the formation of RD within 2 weeks.ConclusionCoating of A5G81 to non‐tissue culture–treated polystyrene facilitates spreading, proliferation and differentiation of hDPCs, resulting in rapid RD formation in artificially exposed pulp.

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Evaluating the potential of an amelogenin-derived peptide in tertiary dentin formation

Cited by 5 publications

References 35 publications

Odontogenesis by Endocytosis of Peptide Embedding Bioactive Glass Composite

Odontogenesis by Endocytosis of Peptide Embedding Bioactive Glass Composite

Amelogenin-Inspired Autoadaptive Peptide in a Caries Microenvironment Facilitates Long-Term Protection of the Dentin–Pulp Complex

LAMA5‐inspired adhesive dodecapeptide facilitates efficient dentine regeneration: An in vitro and in vivo study

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